EVALUATION OF THE EFFECTS OF GOLDEN SILK-DERIVED EXTRACT ON EPIDERMAL BARRIER MARKERS, CASPASE-14 AND RELATED GENES IN HUMAN SKIN EQUIVALENT MODELS.
- TR Number: 742
- Keywords: Caspase-14, elafin, EPI-100-MM-HCF, EPI-201, glucocorticoid receptor, glucocorticoid receptor antagonist, RU-486, Involucrin, profilaggrin, skin barrier, transglutaminase 1
Glucocorticoids are widely used for their anti-inflammatory effects but the mechanism of their action in skin has not been fully understood. Bayo et al reported that glucocorticoid receptor is required for skin barrier competence(1) and Cirillo et al reported that epidermal keratinocyte synthesize and activate cortisol(2). Caspase-14 is a cystenyl-aspartate-specific proteinase that is specifically expressed in epidermal keratinocytes. Dysregulation of caspase-14 expression is implicated in impaired skin barrier formation(3). In the current study, we used reconstructed epidermal model, EPI-201. This 3D tissue consists of normal, human-derived epidermal keratinocytes which have been cultured to form a multilayered, metabolically and mitotically active, differentiating model of the human epidermis. We have characterized the expression of caspase-14 and evaluated the effects of natural compounds on the expression of caspase-14(4).
Reference Application
- Skin irritation
- Toxicity
- Cytokine analysis
- Immulogical research
- Nanoparticle toxicology/penetration
- Respiratory toxicology
- MMPs
- Intestinal Permeation
- Bacterial colonization
- Translational toxicology
- Dry skin
- drug skin compatibility
- bacterial adherence
- Allergenicity
- Antioxidants
- Drug absorption
- Immunologicaal research
- Toxicology
- Skin cancer
- Photoaging
- Electrolyzed Water
- Transbuccal drug delivery
- XtraMild skin mildness testing
- Skin moisturization
- Protein Expression
- PBPK Modeling
- Immunological Research
- Apoptosis
- Intestinal toxicity
- Microbicides
- Nanotoxicology
- Skin corrosion
- Skin Sensitization
- Fibrosis
- Oral Pathology
- bacterial vaginosis
- ADME
- Gastrointestinal Inflammation
- anti-wrinkling
- Immunogenicity
- Basic dermal research
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- Immunotoxicity
- Ocular irritation
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- vaginal microbiome
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- Nicotine pouch products
- Inflammation
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- Oral candidiasis
- Skin lightening
- Organ-on-a-Chip
- Oxidative Stress
- Oral inflammation
- Collagen Remodeling
- Hyperpigmentation
- Barrier Function
- intranasal drug delivery
- Microbial infection
- COPD
- Smoking
- Respiratory toxicity
- Oral irritation
- Skin
- Oral Disease Research
- Skin Damage
- Ocular toxicology
- Drug Screening
- Skin de-pigmentation
- Gastrointestinal Toxicity
- permeation enhancement
- Wound healing
- Smoke
- Tobacco
- Inhalation Toxicology
- Oral mucositis
- Smoker
- Space Research
- Skin Barrier
- Validation
- Nephrotoxicity
- Cancer Research
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- excipients
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- Irritation>Eye Irritation OECD TG 492
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- pesticides
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- Respiratory infection
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