Anti-Aging

Examine changes in extracellular matrix (ECM) gene and protein expression and histological structure to determine the anti-aging efficacy of cosmetic ingredients or final formulation. EpiDermFT is available from a range of adult donors.

METHODS

EpiDermFT tissues (Figure 1) are equilibrated overnight under standard culture conditions (37C, 5% CO2) with EpiDermFT Assay Media (EFT-400-ASY). 18-24 hours later tissue culture supernatants are aspirated and replaced with fresh, pre-warmed media. EpiDermFT is exposed to test formulations or active ingredients for 24-72 hours. Following exposure, EpiDermFT is harvested for RNA isolation, protein isolation or histological/immunohistochemical evaluation.

EFT Histology1RESULTS

EpiDermFT tissues treated with Formulations A showed significant increases in Collagen 1A1, Collagen 3A1 and Elastin gene expression.  Tissues treated Formulation B resulted in significant increases in Collagen 3A1 expression (Figure 2).

Anti-Aging Graph

Additional anti-aging biomarkers include MMPs, TIMPs, Pro-collagen, Hyaluronic Acid, Inflammatory Mediators, etc.

CONCLUSIONS

Evaluation of ECM components in the EpiDermFT in vitro human 3D skin model allows for the rapid and cost-effective assessment of topically applied formulations or active ingredients for efficacy and claims substantiation studies.

Download the EpiDermFT Anti-Aging Application Note.

For more information, view Technical References.

Learn more about EpiDermFT.