Eye Irritation Test (OECD TG 492)

The EpiOcular Eye Irritation Test predicts the acute eye irritation potential of a topically applied chemical or formulation by measurement of its cytotoxic effect of the EpiOcular cornea epithelial model. This in vitro hazard assessment allows for the identification of chemicals (substances and mixtures) not requiring classification and labeling for eye irritation or serious eye damage in accordance with UN GHS.

MatTek offers the EpiOcular Eye Irritation Test as a GLP or non-GLP service.

Protocol

Test ModelEpiOcular
ReplicatesN=2 tissues per test condition
Exposure TimeLiquids – 30 minute topical exposure to 50µl of test material per tissue
Solids  – 6 hour topical exposure to 50mg of test material per tissue
Assay ControlsNegative Control – Sterile deionized H2O
Positive Control – Methyl Acetate
EndpointsMTT Tissue Viability Assay
Data Delivery% relative viability ± SD
In vitro classification

Download the EpiOcular Eye Irritation Test Protocol

Data

Tissue-Viability-Table

EIT-Viability-Graph

References

Kaluzhny, Y., et al, (2015). EpiOcularTM  Eye Irritation Test (EIT) for Hazard Identification and Labeling of Eye Irritating Chemicals: Protocol Optimization for Solid Materials and Extended Shipment Times. Altern Lab Anim. 2015 May;43(2):101-27. 

OECD (2015), Test No. 492: Reconstructed human Cornea-like Epithelium (RhCE) test method for identifying chemicals not requiring classification and labeling for eye irritation or serious eye damage, OECD Publishing, Paris. DOI: http://dx.doi.org/10.1787/9789264242548-en

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