Measure the electrical impedance of EpiDerm or EpiDermFT to assess the efficacy of topically applied moisturizers including creams, gels and lotions. The Skin Hydration protocol allows for data analysis within 24 hours.
EpiDerm or EpiDermFT tissues (Figure 1) are equilibrated overnight under standard culture conditions (37°C, 5% CO2) with EpiDerm or EpiDermFT Assay Media (EPI-100-NMM, EFT-400-ASY). 18-24 hours later tissue culture supernatants are aspirated and replaced with fresh, pre-warmed media. Tissues are exposed to test formulations for 1-4 hours hours. Following exposure, skin hydration is evaluated by measuring the electrical impedance of the tissue surface (DPM 9003 Nova Meter, Nova Technologies).
Skin hydration levels were significantly higher in tissues treated with Formulation 1, Formulation 2 and Formulations 3 compared to untreated controls. * Student’s t-test (P ≤ 0.01). Data is presented as experimental replicates (n=4).
Assessment of skin hydration following treatment with topically applied formulations and moisturizers in the EpiDerm and EpiDermFT in vitro human skin models can be used in product efficacy and claims substantiation studies.
Download the Skin Hydration Application Note.
For more information, view Technical References.
Learn more about EpiDerm.
Learn more about EpiDermFT.