FAQ’s

Glass Bottom Cultureware

Q1: How are glass bottom dishes typically used?
Q2: What type of glass bottom dish should I use to grow my cells?
Q3: How can I search for published methods utilizing MatTek's glass bottom dishes?
Q4: What should I do if my cells won't grow or if I want to improve growth in the glass bottom dishes?
Q5: Can the coverslip be removed from the glass bottom dish?
Q6: How can I control the temperature of the glass bottom dishes for in situ (live) microscopy?
Q7:Why would one want to use glass bottom dishes containing gridded coverslips? What is the size of the grid? What if I can't see the grid?
Q8:How can I use the glass bottom dishes to look at tissue slices?
Q9: Can the glass bottom dishes be re-used?

Microscopy Types and Techniques

Q10: Are the glass bottom dishes good for confocal and fluorescence microscopy?
Q11: How can I use the glass bottom dishes for Nomarski Differential Interference Contrast (DIC) microscopy?
Q12: Can the dishes be used for high-resolution techniques such as GSDIM, dSTORM, PALM , or TIRF microscopy?
Q13: Why are the 50 mm glass bottom dishes useful for microinjection and maintaining a constant atmosphere in the dish?
Q14:How can I perfuse the cells growing in the glass bottom dishes?

Dish Properties

Q15: What coverslip thickness should I use? What do the various coverslip numbers correspond to?
Q16: What is the adhesive used to attach the coverslips to the petri dishes?
Q17: How long can the dishes be stored?
Q18: Over what temperature range can the glass bottom dishes be used?
Q19: What are properties of the glass used in the glass bottom dishes?
Q20: How deep is the micro-well of the glass bottom dish?
Q21: What is the chemical compatibility of Glass Bottom Dishes and Multi-Well Plates? Can they be used with organic solvents?
Q22: How do I know that the glass bottom dishes are sterile?
Q23: How do the high tolerance P35G-0.170-14-C dishes differ from the standard P35G-1.5-14-C? For what applications would the P35G-0.170-14-C dishes be useful?

Coatings

Q24: What is the molecular weight range of the poly-d-lysine used to coat the PDL coated glass bottom dishes?
Q25: Why are the dishes coated with poly-d-lysine (instead of poly-l-lysine)?
Q26: What type of collagen is applied to the collagen coated glass bottom dishes (part #: P35GCol-x-xx-C or P50GCol-x-xx-F)?
Q27: Do the poly-lysine or collagen coatings affect the optical properties of the glass bottom dishes?

Special Orders

Q28: Other than the standard 10 mm, 14 mm, and 20 mm diameter glass surfaces, do the glass bottom dishes come with any alternative diameters?
Q29: What is the lead-time necessary for special orders? Is there a special order charge? Can special order items be returned?
Q30: What are the advantages of using multi-well glass bottom plates compared to the standard glass bottom culture dishes?
Q31: Do you offer multi-well glass bottom plates that are black?

3D Tissue Models

Q1: How long has MatTek been in business?
Q2: What are MatTek's major product lines?
Q3: How many different types of in vitro human tissue equivalents does MatTek produce?
Q4: What are the typical configurations for these tissues?
Q5: Does MatTek produce any other in vitro products?
Q6: Do any of the in vitro human tissue equivalents also contain Dendritic/Langerhans Cells?
Q7: What are some typical applications for MatTek's in vitro human tissue equivalents?
Q8: What do these in vitro human tissue equivalents cost?
Q9: Can any of the in vitro tissues be used on humans?
Q10: Do you have dealers or distributors for your in vitro products?
Q11: I've never used your in vitro human tissue equivalents—does MatTek provide free samples to test?
Q12: How are the in vitro human tissue equivalents shipped?
Q13: Do you ship in vitro tissues outside the USA? Are there any special regulations or paperwork that needs to be filled out?
Q14: We have products that need to be tested, but don't have the on-site expertise to perform these tests? Will MatTek perform the tests for us?
Q15: Are there detailed protocols that I can follow to use MatTek in vitro human tissue equivalents?
Q16: What is the significance of each of the following terms as they apply to MatTek's in vitro human tissue equivalents?
Q17: What is the single most important feature of MatTek in vitro human tissue equivalents?
Q18: What is the best way to get specific information about my in vitro application?
Q19: What are all of these TR's referenced on your Web site?
Q20: What is the quickest method to see if MatTek has information related to a specific Application, Keyword, Individual, or Organization?
Q21: What are ICCVAM and ECVAM and why do I see them referenced on the MatTek Web site?
Q22: What is a 'validated' in vitro method? Do MatTek in vitro human tissue equivalents have validated methods?
Q23: What is the MTT Assay and what is it used for?
Q24: Can any MatTek in vitro human tissue equivalents be used in Transdermal Drug Delivery studies?
Q25 Can any MatTek in vitro human tissue equivalents be used in Inhaled / Nasal Drug Delivery studies?
Q26: Where is the 'Glass Bottom Dish' Product Line information on the MatTek Web site?