CONFOCAL MICROSCOPY FOR MODELING ELECTRON MICROBEAM IRRADIATION OF SKIN.

• TR Number: 691
• Authors: Miller1, J.H., Chrisler2, W. B., Wang3, X., Sowa2, M.B. 1School of Electrical Engineering and Computer Science, Washington State University Tri-Cities, Richland, WA 99354, USA. 2Cell Biology and Biochemistry, Pacific Northwest National Laboratory, Richland, WA 99354, USA. 3Biological Monitoring and Modeling, Pacific Northwest National Laboratory, Richland, WA 99354, USA.
• Materials Tested: Electron microbeams, Microbeams, Particle microbeams

For radiation exposures employing targeted sources such as particle microbeams, the deposition of energy and dose will depend on the spatial heterogeneity of the sample. Although cell structural variations are rela- tively minor for two-dimensional cell cultures, they can vary significantly for fully differentiated tissues. Employ- ing high-resolution confocal microscopy, we have deter- mined the spatial distribution, size, and shape of epidermal keratinocyte nuclei for the full-thickness EpiDermTM  skin model (Mattek, Ashland, VA). Application of these data to calculate the microdosimetry and microdistribution of energy deposition by an electron microbeam is discussed.