DEVELOPMENT OF AN IN VITRO METHOD FOR SCREENING ANTI-IRRITANTS.
- TR Number: 170
- Authors: Majmudar, G., Smithey, T., Fisher, L. Mary Kay Holding Corp.
The development of an in vitro method for testing unknown anti-irritant ingredients can provide preliminary estimate of material safety and it is fast and economical before testing on humans. We utilized the Mattek EpiDerm skin model and a primary culture of keratinocytes to screen a variety of anti-irritants. EpiDerm partially mimics human skin morphologically and biochemically, and therefore, it was possible to test anti-irritants in the product. Applying known irritants like sodium lauryl sulfate, lactic acid and UV light to EpiDerm or keratinocytes resulted in inhibition of mitochondrial enzyme activity and release of interleukins and prostaglandins. Cytokines, prostaglandins and mitochondrial enzyme MTT assays were conducted for anti-irritant screening following pre-application of anti-irritant or an otherwise potentially irritating formula which also contained an anti-irritant. Anti-irritants such as green tea and natural extracts reduced the release of cytokines and prostaglandin and protected mitochondrial enzyme. Clinical data on the effect of green tea and natural extracts on SLS and lactic acid irritation showed good agreement with the in vitro tests.
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- Inflammation
- Asthma
- Skin corrosion Absorption
- Mucosal
- Oral candidiasis
- Skin lightening
- Organ-on-a-Chip
- Oxidative Stress
- Oral inflammation
- Collagen Remodeling
- Hyperpigmentation
- Barrier Function
- Microbial infection
- COPD
- Smoking
- Respiratory toxicity
- Oral irritation
- Skin
- Oral Disease Research
- Skin Damage
- Ocular toxicology
- Drug Screening
- Skin de-pigmentation
- Gastrointestinal Toxicity
- Wound healing
- Smoke
- Tobacco
- Inhalation Toxicology
- Oral mucositis
- Smoker
- Space Research
- Skin Barrier
- Validation
- Nephrotoxicity
- Cancer Research
- Skin Brightening
- Phototoxicity
- Research
- Drug development
- Nanoparticles
- Skin pigmentation
- Gingivitis
- Dry Eye
- Drug Metabolism
- Biofilm
- Hepatotoxicity
- Personalized Medicine
- Food Additives
- UV radiation
- Basic cutaneous research
- Epithelial restitution
- Microbial
- Pigmentation
- Transbuccal permeation/penetration
- Micronucleus Assay
- Skin aging
- Intestinal barrier
- Liver Toxicity
- Consumer products
- Anti-aging
- Basic DC research
- Eye irritation
- Microbicide
- Pigmentation studies
- Oral mucosa
- microbiome
- Skin disease
- SARS-CoV-2
- Skin Toxicity
- Cytotoxicity
- Skin hydration
- UV
- Genetic toxicology
- Microbicide testing
- Radiation
- Tumor invasion
- Probiotic
- Intestinal infection
- Skin re-epithelization
- Crohn's Disease
- Inflammatory response
- UV toxicity
- Basic respiratory research
- Genomics
- STD infection
- Reproducibility - eye (ocular) tissue model
- UV light
- Irritation>Eye Irritation OECD TG 492
- Skin differentiation
- Barrier repair
- Inflamed Bowel Disease
- Visible Light
- Absorption
- Antimicrobial
- Buccal delivery
- Genotoxicity
- Mucosal delivery
- Reproducibility - skin tissue models
- UV protection
- Corneal Drug Delivery
- UV damage
- transporters
- Regulatory Approval
- Review Article
- Drug delivery
- Infectious disease research
- Buccal drug delivery
- Pharmacotoxicology
- Nasal absorption
- Nanotechnology
- Aging
- Respiratory Disease
- Bacterial infection
- Pollution
- Mildness Testing
- Barrier Disruption
- Irritation
- Infection
- Mucosal irritation
- Psoriasis
- Vaginal irritation
- Respiratory immunotoxicity
- Human-on-a-chip
- Atopic Dermatitis
- DNA Damage
- Colitis
- Drug ADME
- Hair Growth
- Permeation
- Infections
- Cosmetics
- Metabolism
- Mucous
- Respiratory infection
- Viral Infection
- Melanogenesis
- Antiviral
- Gastrointestinal Disease
- Hazard assessment
- Biomedical Devices
- Skin irritation
- Toxicity
- Cytokine analysis
- Immulogical research
- Nanoparticle toxicology/penetration
- Respiratory toxicology
- MMPs
- Intestinal Permeation
- Bacterial colonization
- Translational toxicology
- Dry skin
- drug skin compatibility
- Allergenicity
- Antioxidants
- Drug absorption
- Immunologicaal research
- Toxicology
- Skin cancer
- Photoaging
- Electrolyzed Water
- Transbuccal drug delivery
- XtraMild skin mildness testing
- Skin moisturization
- Protein Expression
- Immunological Research
- Apoptosis
- Intestinal toxicity
- Microbicides
- Nanotoxicology
- Skin corrosion
- Skin Sensitization
- Fibrosis
- Oral Pathology
- bacterial vaginosis
- ADME
- Gastrointestinal Inflammation
- Immunogenicity
- Basic dermal research
- Respiratory research
- Immunotoxicity
- Ocular irritation
- Penetration
- Medical Devices
- Pulmonary Fibrosis
- Oral infection
- vaginal microbiome
- Microphysiological system
- Gastrointestinal Irritation
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