RANTES, GRO-α, TNF-α RELEASE FROM IN VITRO EPIDERMIS MODELS FOLLOWING TREATMENT OF STAPHLOCOCCAL ENTEROTOXIN A.

• TR Number: 208
• Authors: Yamada, H., Yudate, T., Matukura, M., Tezuka, T. University School of Medicine, Osaka, Japan.

Human skin equivalent cultures are currently being investigated as in vitro models to predict the cytokine expression. The object of the research presented here is to evaluate the production of panel of immunomodulatory cytokines under the Staphylococcus infection and to determine the modulation of these cytokines following exposure to Staphylococcal enterotoxin in the Mattek EpiDerm model. EpiDerm cultures were topically treated with Staphylococcus aureus, SEA, SEA with several cytokine, assay medium, or no treatment for 24 hours. TNF-a, GRO-a, RANTES were measured by ELISA. Treatment with SEA produced increase of TNF-a, GRO-a but not RANTES. SEA With IFN-g produced RANTES. These studies demonstrate S.aureus modulate cytokine production from keratinocyte.