DOPAMINE TRANSPORT ACROSS RESPIRATORY EPITHELIUM.

• TR Number: 314
• Keywords: Amantadine HCl, Bovine respiratory explants, CNS, Catecholamine, DAT , Diffusion chamber, Dopamine (DOPA), Dopamine HCl, Dopamine transporter (DAT), EpiAirway, Flux studies, GBR 12935, Human derived tracheo/bronchial tissue system, Human traceheobronchial cell cultures, Immunohistochemistry, Intranasal, Metabolism, Mucosa, Nasal, Nasal drug delivery, Nasal glands, Neurotransmitter, Nomifensine maleate, OCT, OCT-2, Organic cation transporter (OCT), Permeability, Respiratory, Respiratory mucosa, TEER, Tracheo-bronchial

Dopamine is a catecholamine neurotransmitter implicated in CNS related disorders such as Parkinson’s disease, schizophrenia and Huntington’s disease. It is known to be transported by the dopamine transporter (DAT) in the CNS and an organic cation transporter (OCT) peripherally. It is orally ineffective due to high first pass metabolism and its inability to cross the blood brain barrier. Intranasal dopamine administration has shown preferential uptake into the brain as compared to intravenous administration. Bovine respiratory explants and a novel human derived tracheo/bronchial tissue system were used to investigate the processes underlying dopamine transport.