Comparative evaluation of artificial saliva and complete artificial saliva as solvent vehicles for in vitro toxicity testing of oral tobacco products

• TR Number: 1076
• Authors: Xuefei Cao, Mariana T Farcas, Yevgeniya V Prepelitskaya, Abaigeal Ritzenthaler, Jennifer Molignano, Jonathan Oldach, Marisol M Gutierrez
• Keywords: EpiOral, ORL-200-ORL-300-FT, complete artificial saliva, artificial saliva, nicotine pouches, IL-6, IL-8, 8-Isoprostane, oxidative stress, MTT, Tobacco-specific nitrosamines, TSNAs, CORESTA Smokeless Tobacco Reference Product CRP1.1, Swedish Snus
• Materials Tested: complete artificial saliva, artificial saliva
• Link to Article: https://pmc.ncbi.nlm.nih.gov/articles/PMC12719288/

Oral tobacco-derived nicotine (OTDN) pouches are tobacco-free products and are considered potential reduced-risk alternatives to traditional tobacco products within the tobacco harm reduction framework. Despite their growing popularity, the local oral toxicity profiles of OTDNs remain poorly characterized. Although in vitro toxicity studies have been conducted, the lack of standardized testing protocols, including solvent vehicle selection, limits data comparability across laboratories. To address this, we evaluated and compared the biological effects of artificial saliva (AS) and complete artificial saliva (CAS) across four in vitro oral models to identify an appropriate solvent vehicle for mechanistic testing.