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ENHANCED ANTIVIRAL ACTIVITY OF ACYCLOVIR LOADED INTO NANOPARTICLES.

Cavalli1, R., Donalisio2, M., Bisazza1, A., Civra2, A., Ranucci3, E., Ferruti3, P. and Lembo2, D. 1Dipartimento di Scienza e Tecnologia del Farmaco, Universita` di Torino, Torino, Italy. 2Dipartimento di Scienze Cliniche e Biologiche, Universita` di Torino, Torino, Italy. 3Dipartimento di Chmica Organica e Industriale, Universita` degli Studi di Milano, Milano, Italy.
Abstract

The activity of antivirals can be enhanced by their incorporation in nanoparticulate delivery systems. Peculiar polymeric nanoparticles, based on a β-cyclodextrin-poly (4-acryloylmorpholine) monoconjugate (β-CD-PACM), are proposed as acyclovir carriers. The experimental  procedure  necessary to obtain the acyclovir-loaded nanoparticles  using  the  solvent displacement  preparation  method   will be described   in  this  chapter.   Fluorescent labeled   nanoparticles are prepared using the same method for cellular trafficking studies. The biocompatibility assays necessary to obtain safe nanoparticles are reported. Section 4 of this chapter describes the assessment of the antiviral activity of the acyclovir-loaded nanoparticles.

Keywords

IL-1alpha release, Irritation potential, LDH release, MTT ET-50, VEC-100

Materials Tested

b-CD-PACM nanoparticles

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