DC DETOXDEFENSE™ STIMULATES SKIN’S OWN SOD ANTIOXIDANT PROTECTION AND DETOXIFICATION.
Objective: The objective of this experiment was to determine whether Cordyceps and DC Detox Defense have protective effect against UVB-mediated cytotoxicity in Mattek full thickness skin substitute model. Method: EpiDermFT tissues (cat.#EFT 212) were obtained from Mattek Corp. and cultured according to the manufacturer’s instructions. The test materials – Isoflavones 150 # 45K/1984/E, Cordyceps Extract and DC Detox Defense – were received on the 15th of May 2008 and kept at 4oC. Isoflavones were tested at 0.25% (w:v), while Cordyceps and Detox Defense at 2.5% and 0.5% (vol:vol). Test materials were incubated with skin substitutes for 48h, then the tissues were UVB irradiated (peak at 302nm) with Hoefer Scientific Instruments transilluminator UVTM-19 for 9min. at 0.8mW/cm2. This UVB fluence was equivalent to one minimal erythema dose (MED) in Fitzpatrick type I-IV human skin, equal to 20-25 min of sun exposure during summer time in Central Park, New York City (Moore et al., 2006). The irradiation was monitored with calibrated radiometer and probe (model S370, United Detector Technology). Following the irradiation, skin substitutes were returned to the incubator for 16 hours, afterwhat cell viability was determined using the standard MTT method and compared to the non irradiated control.
Anti-aging skin, Detoxifying, Energizing, EpiDermFT, Fungus, Mushrooms, Phospholipids vesicle, Pollution protection, Sun care, UV toxicity
Beta vulgaris (beet) root extract, Butylene glycol, Cordyceps, Cordyceps extract, DC Detoxdefense, Isoflavones, Phospholipids, Sinensis extract, Soy isoflavones, Sugar beet extract, UVB
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