PARP-1 ACTIVITY IS INVOLVED IN THE SOLAR UV-INDUCED CUTANEOUS INFLAMMATORY RESPONSE IN THE EPIDERM-FT IN VITRO HUMAN SKIN MODEL.

• TR Number: 650
• Authors: Kandarova, H., Armento, A., Stolper, G., Cooney, C., Li, M., and Hayden, P.J. Mattek Corporation, Ashland, MA, USA.
• Materials Tested: 4 amino-1,8-napthalamide, UVA, UVB

Poly (ADP-ribose) polymerase 1 (PARP-1) is known to be activated under conditions of genotoxic stress.  In the presence of DNA damage, PARP-1, a nuclear zinc-finger DNA-binding protein, is activated and catalyzes the transfer of ADP-ribose from NAD+ to itself (automodification) and a range of other nuclear proteins (1,2,3).  UV-induced cutaneous inflammation has been demonstrated in several models and appears to occur through the activation of Nuclear Factor kappa B (NF-кB) (4,5).  In the present study, the EpiDerm-FT in vitro human skin model (EFT-400) was used to gain insight into the mechanisms of the UV-induced inflammatory response of human epidermal tissue.  The EpiDerm-FT model consists of a 3-demensional, highly differentiated epidermis cultured with normal human epidermal keratinocytes cultured on a collagen dermis populated with normal human dermal fibroblasts.  The model exhibits stratified epidermal components and a fully developed basement membrane at the dermal/epidermal junction.