In vitro cytotoxicity assessment and mechanistic insight of EpiOral™ tissue model to oral nicotine products with extraction stability analysis
- TR Number: 1091
- Authors: Emma Rothwell, Adam Seymour, Robell Medhane, Jacob Lawrance, Michael Hollings
- Keywords: EpiOral (ORL-200), oral pouch nicotine products, complete artificial saliva, extraction, tobacco specific nitrosamines, TSNA, NNK, NAT, NNN, NAB, MTT, LDH, 8-isoprostane, IL-12-P70, TNFa, IL-10, IL-6, IL-1b, IL-8, Velo Max Freeze, ZYN CHILI GUAVA, Nordic Spirit, LOOP Smooth mint, CRP1.1, CRP2.1
- Materials Tested: oral pouch nicotine products, complete artificial saliva, extracts, Velo Max Freeze, ZYN CHILI GUAVA, Nordic Spirit, LOOP Smooth mint, CRP1.1, CRP2.1
- Link to Article: https://www.labcorp.com/education-events/posters/vitro-cytotoxicity-assessment-and-mechanistic-insight-epioraltm-tissue-model-oral-nicotine-products
Cell models applicable to the oral route of use are important for analysing cytotoxicity of oral nicotine products. MatTek™ EpiOral tissues were utilised when testing these products in the MTT [3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide] assay. Products were extracted in complete artificial saliva (CAS) to mimic product use. Extractions were performed on 300mg of product per mL in CAS, extracting for 2 hours. Treatments were performed on fresh extractions, 1-month frozen samples and 3-month frozen samples. Extracts were measured for nicotine and tobacco specific nitrosamines (TSNA), and analysis of frozen extracts was performed for each experiment. Lactate dehydrogenase (LDH), 8-isoprostane and cytokines release was measured to gather a mechanistic insight.