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EpiOral

1095

Assessment of cytotoxicity, skin and oral irritation of medical devices with hydrating properties: A case study

Ensuring the biocompatibility of medical devices (MDs) is essential for regulatory compliance and user safety. Under the evolving requirements of the European Medical Device Regulation (EU MDR 2017/745), products that […]
1091

In vitro cytotoxicity assessment and mechanistic insight of EpiOral™ tissue model to oral nicotine products with extraction stability analysis

Cell models applicable to the oral route of use are important for analysing cytotoxicity of oral nicotine products. MatTek™ EpiOral tissues were utilised when testing these products in the MTT […]
1088

In Vitro Oral Cavity Permeability Assessment to Enable Simulation of Drug Absorption

Background/Objectives: The oral cavity represents a convenient route of administration for drugs that exhibit significant hepatic first-pass extraction. In this study, the mucosal permeation properties of selected active pharmaceutical ingredients (APIs) […]
1076

Comparative evaluation of artificial saliva and complete artificial saliva as solvent vehicles for in vitro toxicity testing of oral tobacco products

Oral tobacco-derived nicotine (OTDN) pouches are tobacco-free products and are considered potential reduced-risk alternatives to traditional tobacco products within the tobacco harm reduction framework. Despite their growing popularity, the local […]
1066

Development of an In Vitro Method for Assessing the Potential Irritation of Medical Devices and OTC Products Used in the Oral Cavity

The aim of this study was to evaluate an in vitro method using the EpiOralTM model, a three-dimensional cultured human buccal epithelium, for assessing the oral irritation potential of various products. […]
1063

Exploring the potential of reconstructed human epithelial tissue models for safety assessment of intraoral medical devices

Medical devices are integral to a wide array of medical interventions and are increasingly utilized in both clinical and home settings. Within the oral cavity, intraoral medical devices are employed […]
1055

Sensitivity Analysis and Quality Indicators for an In Vitro Oral Irritation Assay

Biocompatibility testing using in vivo tests is often one of the final evaluations of new dental materials. To reduce the likelihood of failure at this late stage, predictive biocompatibility testing […]
1045

Efficacy and hazards of 425 nm oral cavity light dosing to inactivate SARS-CoV-2

Objective: Using a battery of preclinical tests to support development of a light-based treatment for COVID-19, establish a range of 425 nm light doses that are non-hazardous to the tissues […]
4245

Toxicological Aspects of in vitro Oral Mucosa Wound Healing Models

Wounds in the oral cavity tissues can result from surgical procedures, accidents, and canker sores. Oral wounds present a site for potential infection and increased sensitivity to oral irritants. Untreated […]
1028

In vitro permeation of nicotine and tobacco specific nitrosamines from smokeless tobacco product extracts in a 3D buccal tissue model

Tobacco product use is a risk factor in the development of oral cancer, although epidemiology studies show this risk is far less with smokeless tobacco product use than cigarette smoking. […]
795

Toxicity and Inflammatory Response to Common Magic Mouthwashes in a 3D Oral Reconstructed Tissue Model

Magic Mouthwash is a general term describing oral rinses prescribed by a doctor for the treatment of pain, inflammation, or infection, commonly as a result of chemotherapy and radiation induced […]
1025

Safety assessment of monographed OTC cold/cough medicine using an in vitro testing platform based on human reconstructed oral tissues

Over-the-counter (OTC) products are available to alleviate concurrent symptoms of colds and flu. They are primarily based on a combination of decongestants, antitussive and alpha adrenergic agonists, which are well-established […]
1005

Single-cell analysis of Kaposi’s sarcoma-associated herpesvirus infection in three-dimensional air-liquid interface culture model

The oral cavity is the major site for transmission of Kaposi’s sarcoma-associated herpesvirus (KSHV), but how KSHV establishes infection and replication in the oral epithelia remains unclear. Here, we report […]
995

Development of an oral mucosal irritation test using a three-dimensional human buccal oral mucosal model

 The oral mucosa can become irritated by oral care products and lip cosmetics. Therefore, it is important to determine the irritation potential of their ingredients and products during safety evaluations. […]
993

Oral Absorption across Organotypic Culture Models of the Human Buccal Epithelium after E‑cigarette Aerosol Exposure

Inhaled aerosols are absorbed across the oral cavity, respiratory tract, and gastrointestinal tract. The absorption across the oral cavity, which is one of the exposure routes, plays an important role […]
982

Cytokine Release from Organotypic Human Oral Tissue Following Exposure to Oral Care Product Chemicals

Purpose: Measuring viability of a three-dimensional in vitro organotypic human oral tissue model has been suggested as an alternative test method to the oral mucosa irritation test of oral care […]
971

Cytotoxicity, oxidative stress, and inflammatory response of smokeless tobacco extracts and cytotoxicity of combustible cigarette whole smoke in a 3D oral organotypic buccal cell model

Oral disease is frequently associated with viral and environmental exposures and oral hygiene. The use of tobacco is a risk factor in the development of oral disease. Cytotoxicity, inflammatory response, […]
927

Potent Antiviral Activity against HSV-1 and SARS-CoV-2 by Antimicrobial Peptoids

Viral infections, such as those caused by Herpes Simplex Virus-1 (HSV-1) and SARS-CoV-2, affect millions of people each year. However, there are few antiviral drugs that can effectively treat these […]
919

In vitro three-dimensional organotypic culture models of the oral mucosa

Three-dimensional, organotypic models of the oral mucosa have been developed to study a wide variety of phenomena occurring in the oral cavity. Although a number of models have been developed […]
904

Effects of Epidermal Growth Factor-Loaded Mucoadhesive Films on Wounded Oral Tissue Rafts

Current treatments for traumatic oral mucosal wounds include the gold standard of autologous tissue and alternative tissue engineered grafts. While use of autografts has disadvantages of minimal availability of oral […]
199

Transmucosal Delivery of Nicotine in Combination with Tincture of Benzoin Inhibits Apoptosis

Background and Objective – The aim of this study was to test the hypothesis that tincture of benzoin (TOB) facilitates immediate transmucosal nicotine absorption while simultaneously promoting a safe and […]
885

In Search of Preventative Strategies: Novel Anti Inflammatory High-CBD Cannabis Sativa Extracts Modulate ACE2 Expression in COVID-19 Gateway Tissues

With the rapidly growing pandemic of COVID-19 caused by the new and challenging to treat zoonotic SARS-CoV2 coronavirus, there is an urgent need for new therapies and prevention strategies that […]
868

Functional Characterization and Permeation Studies of Lyophilised Thiolated Chitosan Xerogels for Buccal Delivery of Insulin

Stable and mucoadhesive, lyophilised, thiolated chitosan xerogels, loaded with insulin for buccal mucosa delivery, in place of the currently used parenteral route have been developed. The xerogels were backed with […]
867

Denture-associated biofilm infection in three-dimensional oral mucosal tissue models

Purpose. In vitro analyses of virulence, pathogenicity and associated host cell responses are important components in the study of biofilm infections. The Candida-related infection, denture-associated oral candidosis, affects up to […]
866

Evaluating the Inflammatory and Genotoxic Effects of Smokeless Tobacco Using a Human Organotypic Model of Oral Epithelium

In addition to the well-known effects of tobacco use on the causation of lung and cardiovascular disease, tobacco use is also implicated as a major cause of oral cavity disease […]
861

Evaluation of the Safety, Cell Migration, and Mucoadhesive Properties of a Mucoadhesive Polymer Blend in Human Oral Mucosa

The efficacy of active pharmaceutical ingredients (API) in compounded medications for oral mucosa greatly depends on the composition of the base. Here, we assessed the safety, facilitation of cell migration, […]
860

Evaluation of Clay-Functionalized Wafers and Films for Nicotine Replacement Therapy via Buccal Mucosa

The functional physicochemical properties of nicotine (NIC)-loaded composite freeze-dried wafers and solvent-evaporated films comprising hydroxypropylmethylcellulose (HPMC) and sodium alginate (SA), stabilized with magnesium aluminium silicate (MAS), have been reported. The […]
859

Novel antioxidants are not toxic to normal tissues but effectively kill cancer cells

Free radicals are formed as a result of cellular processes and play a key role in predisposition to and development of numerous diseases and of premature aging. Recently, we reported […]
855

Behaviour of Silver Nanoparticles in Simulated Saliva and Gastrointestinal Fluids

Continuously increasing application of silver nanoparticles (AgNPs) requires information on their safety and performance under biological conditions. Assessment of AgNPs in biological systems is also related to availability of robust […]
831

Oral Irritation Assessment of Electronic Liquids using an In-Vitro Oral Testing Model

While data are still being collected and analyzed, there were at least 1,300 accidental electronic liquid (e-liquid) exposures reported as of 2013. Deaths have occurred as a result of ingestion […]
811

Systems Toxicology Assessment of the Biological Impact of a Candidate Modified Risk Tobacco Product on Human Organotypic Oral Epithelial Cultures

Cigarette smoke (CS) has been reported to increase predisposition to oral cancer and is also recognized as a risk factor for many conditions including periodontal diseases, gingivitis, and other benign mucosal disorders. Smoking […]
842

In vitro systems toxicology approach to investigate the effects of repeated cigarette smoke exposure on human buccal and gingival organotypic epithelial tissue cultures

Smoking has been associated with diseases of the lung, pulmonary airways and oral cavity. Cytologic, genomic and transcriptomic changes in oral mucosa correlate with oral preneoplasia, cancer and inflammation (e.g. periodontitis). Alteration […]
3290

Transcriptional profiling of radiation damage and preventive treatments in a 3-dimensional (3D) human cell culture model of oral mucositis

Cancer patients who receive radiation are often afflicted by oral mucositis, a debilitating disease, characterized by mouth sores and difficulty in swallowing. Oftentimes, cancer patients afflicted with mucositis must stop lifesaving therapies. […]
715

NUCLEOTIDE EXCISION REPAIR IS REDUCED IN ORAL EPITHELIAL TISSUES COMPARED WITH SKIN.

Ultraviolet radiation (UVR) exposure to internal tissues for diagnostic, therapeutic and cosmetic procedures has increased dramatically over the past decade. The greatest increase in UVR exposure of internal tissues occurs […]
450

METHODS FOR ASSESSMENT OF PROLIFERATION IN AN IN VITRO TISSUE MODEL OF ORAL EPITHELIA.

This study by scientists at the University of Colorado and Premier Laboratory, LLC demonstrated that a robust method for the assessment of the proliferative effects of agents applied to Mattek’s […]
773

Effects of ozone nano-bubble water on periodontopathic bacteria and oral cells – in vitro studies

The aims of the present study were to evaluate the bactericidal activity of a new antiseptic agent, ozone nano-bubble water (NBW3), against periodontopathogenic bacteria and to assess the cytotoxicity of […]
551

HIV TYPE 1 FAILS TO TRIGGER INNATE IMMUNE FACTOR SYNTHESIS IN DIFFERENTIATED ORAL EPITHELIUM.

This study by scientists at Prince of Songkla University (Thailand), the University of Washington, Fred Hutchinson Cancer Research Center and Mattek Corp. demonstrated that Mattek’s EpiOral in vitro 3-D human […]
395

BENZO[A]PYRENE-INDUCED ORAL CARCINOGENESIS AND CHEMOPREVENTION – STUDIES IN BIOENGINEERED HUMAN TISSUE.

This study by scientists at the University of South Carolina, the Medical University of South Carolina and Mattek Corp. demonstrated that studies based on Mattek’s EpiGingival in vitro human gingival […]
507

DEVELOPMENT OF AN EPIORAL IN VITRO HUMAN TISSUE MODEL FOR ORAL IRRITANCY TESTING.

This study by researchers at British American Tobacco and Advanced Technologies Ltd. demonstrated that Mattek’s EpiOral in vitro human buccal (inner cheek) tissue equivalent is an excellent in vitro model […]
263

GINGIVAL TISSUE MODEL FOR PREDICTION OF IRRITANCY OF ORAL CARE PRODUCTS.

The development of human reconstructed tissues of the oral cavity that express in vivo-like differentiated characteristics would be important to better understand and develop therapies for gum disease, oral cancer, […]
688

UPDATE ON VALIDATION STATUS AND INDUSTRY UTILIZATION OF NORMAL HUMAN 3D (NHU-3D) ANIMAL ALTERNATIVE MODELS.

Progress in in vitro tissue engineering over the last several decades has contributed to a significant decrease in animal use for industrial risk and safety assessment of products and ingredients. […]
777

Molecular Signatures in the Prevention of Radiation Damage by the Synergistic Effect of N-Acetyl Cysteine and Qingre Liyan Decoction, a Traditional Chinese Medicine, Using a 3-Dimensional Cell Culture Model of Oral Mucositis

Qingre Liyan decoction (QYD), a Traditional Chinesemedicine, andN-acetyl cysteine (NAC) have been used to prevent radiatio ninduce dmucositis.Tiswork evaluates the protectivemechanisms ofQYD,NAC, and their combination(NAC-QYD) at the cellular and transcriptional […]
632

IDENTIFYING CELL AND MOLECULAR STRESS AFTER RADIATION IN A THREE-DIMENSIONAL (3-D) MODEL OF ORAL MUCOSITIS.

Mucositis is a debilitating adverse effect of chemotherapy and radiation treatment. It is important to develop a simple and reliable in vitro model, which can routinely be used to screen […]
667

EVALUATION OF TISSUE ENGINEERED MODELS OF THE ORAL MUCOSA TO INVESTIGATE ORAL CANDIDIASIS.

Candida albicans is a commensal organism that can be isolated from the majority of healthy individuals. However, in certain susceptible individuals C. albicans can become pathogenic leading to the mucocutaneous […]
379

EPIGINGIVAL™ (GIN-100) TISSUE MODELS FOR ORAL IRRITATION STUDIES.

This study by scientists at the University of Washington, University of Iowa and Mattek Corp. demonstrated that Mattek’s EpiOral and EpiGingival in vitro human oral tissue equivalents can be used […]
360

NOVEL IN VITRO METHODOLOGY EVALUATING POTENTIAL MUCOSAL IRRITATION OF ORAL CARE FORMULATIONS.

This study by researchers at The Procter & Gamble Co. demonstrated that Mattek’s EpiOral in vitro human buccal (inner cheek) tissue equivalent provided a quick, reproducible method for evaluating the […]
399

CHARACTERIZATION AND TESTING OF NEW BUCCAL AND GINGIVAL TISSUE MODELS.

This study by scientists at the University of Washington, University of Iowa and Mattek Corp. demonstrated that Mattek’s EpiOral and EpiGingival in vitro human buccal and gingival oral tissue equivalents […]
778

Evaluation of a Reconstructed Human Oral Buccal Tissue Model as a Testing Platform for Determining the Oral Irritation Potential of Tobacco Products

There is an increasing need by the tobacco industry to evaluate the irritation and inflammation potential of tobacco products to support product development goals, for sound product stewardship, and likely […]
757

ORGANOTYPIC HUMAN ORAL TISSUE MODELS FOR EVALUATION OF ORAL CARE PRODUCTS.

Oral irritation can result from exposure of the soft tissue to dentifrice and oral rinse formulations. In vitro tissue models (Mattek Corporation) produced from normal human oral keratinocytes were exposed […]
838

SAFETY EVALUATION OF NANOSILVER USING RECONSTRUCTED HUMAN GIT TISSUES

In our pilot study focused on safety evaluation of nanosilver three novel commercially available  reconstructed human  tissues were used for mimicking the human gastrointestinal tract (GIT). The evaluated endpoints included […]
363

DEVELOPMENT OF A HUMAN ORAL EPITHELIAL TISSUE MODEL TO STUDY HIV TRANSMISSION AND THE ROLE OF BETA-DEFENSINS.

This study by scientists at the University of Washington and Mattek Corp. demonstrated that Mattek’s EpiOral in vitro human buccal (inner cheek) tissue equivalent can be used for studying the […]
292

HUMAN β-DEFENSIN EXPRESSION IN A NEW BUCCAL EPITHELIAL TISSUE MODEL.

Oral epithelia function as microbial barriers against bacteria and yeast that are resident in the oral cavity. These microbes can be categorized as non-pathogenic (commensal) and pathogenic (resulting in cavities, […]
386

EXPRESSION OF BETA-DEFENSINS IN RESPONSE TO HIV-1 IN AN ORAL TISSUE MODEL VS MONOLAYER CULTURES.

This study by researchers at the University of Washington and the Fred Hutchinson Cancer Research Center demonstrated that Mattek’s EpiOral in vitro human buccal (inner cheek) tissue equivalent can be […]
438

ORGANOTYPIC HUMAN ORAL TISSUE MODELS FOR TOXICOLOGICAL STUDIES.

This study by researchers at Procter & Gamble Co., the University of Iowa and Mattek Corp. demonstrated that Mattek’s EpiOral and EpiGingival human oral cavity tissue equivalents can be used […]
429

A HUMAN ORAL TISSUE MODEL TO EVALUATE TOXICITY AND ANTI-HIV ACTIVITY OF THE SPERMICIDE-MICROBICIDE, NONOXYNOL-9M.

This study by researchers at the University of Washington, the Fred Hutchinson Cancer Research Center and Mattek Corp. demonstrated that a new form of Mattek’s EpiOral full thickness human buccal […]
621

GENOTOXICITY TESTING USING THE NORMAL HUMAN CELL BASED 3D EPITHELIAL MODELS.

Safety assessment of new products for human use requires genotoxicity testing to ensure their non-carcinogenicity. Current in vitro genotoxicity assays have low specificity resulting in unacceptably high rates of false […]
607

TRANSDERMAL AND BUCCAL DELIVERY OF METHYLXANTHINES THROUGH HUMAN TISSUE IN VITRO.

This study by researchers at Rutgers University (USA) and the University of Strathclyde (Scotland) demonstrated that Mattek’s EpiOral in vitro human buccal (inner cheek) tissue equivalent is an excellent in […]
670

IN VITRO EFFECTS OF ETHANOL AND MOUTHRINSE ON PERMEABILITY IN AN ORAL BUCCAL MUCOSAL TISSUE CONSTRUCT.

The current study investigated the influence of ethanol and ethanol-containing mouthrinses on model chemical permeability in an in vitro oral buccal mucosal construct (EpiOral, ORL-200, Mattek). Innate ethanol transport and […]
424

ANTIMICROBIAL BARRIER OF AN IN VITRO ORAL EPITHELIAL MODEL.

This study by researchers at the University of Washington demonstrated that Mattek’s EpiOral human buccal tissue equivalent has an in vivo-like antimicrobial barrier and is therefore a superior in vitro […]