SAFETY EVALUATION OF NANOSILVER USING RECONSTRUCTED HUMAN GIT TISSUES
In our pilot study focused on safety evaluation of nanosilver three novel commercially available reconstructed human tissues were used for mimicking the human gastrointestinal tract (GIT). The evaluated endpoints included tissue viability, cytokine release and penetration of silver into the tissue culture medium. The results conﬁrmed no adverse effect of nanosilver on the viability of the tissues even after exaggerated exposure. Penetration of nanosilver through the tissues, probably in the form of Ag ions, was conﬁrmed by ICP-MS in a rate depending on the tissue type and application vehicle. No signiﬁcant (at least two-fold) increase of the inﬂammatory cytokines was recorded by ELISA method. The tested AgNP samples did not elicit any adverse effects in the available reconstructed human GIT tissues.
SMI-100, SMI-100-FT, IL-1a, TNF-a, IL-6, IL-8, MTT viability, permeation, penetration, EpiIntestinal, EpiIntestinalFT, Human GIT tissues
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