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Nanoparticle toxicology/penetration

1060

Assessing nanotoxicity of food-relevant particles: A comparative analysis of cellular responses in cell monolayers versus 3D gut epithelial cultures

Engineered nanoparticles (NPs) are extensively used in the food industry, yet safety concerns remain. The lack of validated methodologies is a bottleneck towards resolving this uncertainty. Hence, the current study aims to compare two cell models by examining the toxicological impacts of two food-relevant NPs (SiO2 and Ag) on intestinal epithelia using monolayer Caco-2 cells and full-thickness […]
1041

Mucoadhesive chitosan-poly (lactic-co-glycolic acid) nanoparticles for intranasal delivery of quetiapine – Development & characterization in physiologically relevant 3D tissue models

Quetiapine hemifumarate (QF) delivery to the CNS via conventional formulations is challenging due to poor solubility and lower oral bioavailability (9 %). Similarly, many other second-generation antipsychotics, such as olanzapine, clozapine, and paliperidone, have also shown low oral bioavailability of <50 %. Hence, the present work was intended to formulate QF-loaded biodegradable PLGA-NPs with appropriate […]
1020

Assessment of Carbon Nanotubes on Barrier Function, Ciliary Beating Frequency and Cytokine Release in In Vitro Models of the Respiratory Tract

The exposure to inhaled carbon nanotubes (CNT) may have adverse effects on workers upon chronic exposure. In order to assess the toxicity of inhaled nanoparticles in a physiologically relevant manner, an air–liquid interface culture of mono and cocultures of respiratory cells and assessment in reconstructed bronchial and alveolar tissues was used. The effect of CNT4003 […]
947

In vitro Impact Preliminary Assessment of Airborne Particulate from Metalworking and Woodworking Industries

Inhalation is the main route of exposure to airborne pollutants. To evaluate the safety and assess the risks of occupational hazards different testing approaches are used. 3D airway epithelial tissues allow to mimic exposure conditions in vitro, generates human-relevant toxicology data, allows to elucidate the mode of action of pollutants. Gillian3500 pumps were used to […]
935

Particulate Matter Decreases Intestinal Barrier-Associated Proteins Levels in 3D Human Intestinal Model

Background: The gastrointestinal tract (GI) tract is one of the main organs exposed to particulate matter (PM) directly through ingestion of contaminated food or indirectly through inhalation. Previous studies have investigated the effects of chronic PM exposure on intestinal epithelia in vitro using Caco−2 cells and in vivo using mice. In this study, we hypothesized […]
908

Dicationic Amino Substituted Gemini Surfactants and their Nanoplexes: Improved Synthesis and Characterization of Transfection Efficiency and Corneal Penetration In Vitro

Purpose- To formulate and characterize nanoparticles from m-7NH-m gemini surfactants, synthesized by a new improved method, for non-invasive gene delivery including optimization of composition for transfection efficiency and corneal penetration. Methods – A one-pot, solvent-free, DMAP-free method was developed for the synthesis of m-7NH-m (m=12– 18) gemini surfactant series. Lipoplexes (LPXs) and nanoplexes (NPXs) of […]
858

Repeated long-term exposures of multi-walled carbon nanotubes to the 3D human lung model EpiAlveolar to predict the onset of fibrosis

Multi-walled carbon nanotubes (MWCNTs) with their extraordinary properties (e.g. stiffness, electrical conductivity, etc.) are among the most commonly used nanomaterials [1]. Human exposure to MWCNTs can occur throughout their life-cycle via inhalation [2,3], causing potential adverse effects, such as pulmonary fibrosis as was shown in animal experiments [4]. Therefore, there is a need to design […]
855

Behaviour of Silver Nanoparticles in Simulated Saliva and Gastrointestinal Fluids

Continuously increasing application of silver nanoparticles (AgNPs) requires information on their safety and performance under biological conditions. Assessment of AgNPs in biological systems is also related to availability of robust toxicological methods for evaluation of toxic potential of AgNPs and information on their physicochemical state. Silver nanoparticles were subjected to action of simulated saliva, gastric […]
852

Genetic toxicity assessment of engineered nanoparticles using a 3D in vitro skin model (EpiDerm™)

Background: The rapid production and incorporation of engineered nanomaterials into consumer products alongside research suggesting nanomaterials can cause cell death and DNA damage (genotoxicity) makes in vitro assays desirable for nanosafety screening. However, conflicting outcomes are often observed when in vitro and in vivo study results are compared, suggesting more physiologically representative in vitro models […]
684

USE OF NORMAL HUMAN 3D (NHU-3D) TISSUE MODELS (EPIDERM™, EPIAIRWAY™) FOR NANOTOXICOLOGY APPLICATIONS

Nanomaterials are increasingly utilized in numerous commercial applications where dermal contact, inhalation, or oral ingestion is likely. However, their toxicological properties are largely unknown. Potential adverse effects of nanoparticle exposure include allergenicity, cytotoxicity, and genotoxicity. Nanomaterials may enter the body by interacting with, and eventually crossing, epithelial barriers including skin, airway, and intestinal epithelium. Once […]
775

SAFETY EVALUATION OF NANOSILVER USING RECONSTRUCTED HUMAN GIT TISSUES

In our pilot study focused on safety evaluation of nanosilver three novel commercially available  reconstructed human  tissues were used for mimicking the human gastrointestinal tract (GIT). The evaluated endpoints included tissue viability, cytokine release and penetration of silver into the tissue culture medium. The results confirmed no adverse effect of nanosilver on the viability of […]