In vitro Impact Preliminary Assessment of Airborne Particulate from Metalworking and Woodworking Industries

Ilona Pavlovska, Anna Ramata‑Stunda, Zanna Martinsone, Martins Boroduskis, Liene Patetko, Inese Martinsone, Anita Seile & IvarsVanadzins

Inhalation is the main route of exposure to airborne pollutants. To evaluate the safety and assess the risks of occupational hazards different testing approaches are used. 3D airway epithelial tissues allow to mimic exposure conditions in vitro, generates human-relevant toxicology data, allows to elucidate the mode of action of pollutants. Gillian3500 pumps were used to collect the airborne particulate from woodworking and metalworking environments. EpiAirway tissues were used to model half working day (4 h), full working day (8 h), and 3 working day exposures to occupational pollutants. Tissue viability was assessed using an MTT assay. For preliminary assessment, RT-qPCR analyses were performed to analyze the expression of gelsolin, caspase-3, and IL-6. Tissue morphology was assessed by hematoxylin/eosin staining. An effect on the proliferation of lung epithelial cell line A549 was assessed. Acute exposure to workspace pollutants slightly affected tissue viability and did not change the morphology. No inhibiting effect was observed on the proliferation of A549 cells. Preliminary analysis showed that both types of particles suppressed the expression of gelsolin, with the effect of metalworking samples being more pronounced. A slight reduction in caspase-3 expression was observed. Particles from metalworking suppressed IL-6 expression


EpiAirway (AIR-100), airborne pollutants, airborne particulate, gelsolin, caspase-3, IL-6, wood processing, metalworking, Zn, Si, Fe, Ca, Mn, Mo, Cr, iron oxide nanoparticles, silica spheres, zinc oxide tubes, shielded metal arc welding, milling

Materials Tested

Zn, Si, Fe, Ca, Mn, Mo, Cr, iron oxide nanoparticles, silica spheres, zinc oxide tubes

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