Tissue Models
DEVELOPMENT OF A NEW IN VITRO TEST FOR DERMAL PHOTOTOXICITY USING A MODEL OF RECONSTITUTED HUMAN EPIDERMIS (EPIDERM).
- TR Number: 155
- Keywords: Chemicals, photo and non-phototoxic, Cytotoxicity, Dermal phototoxicity, EpiDerm, MTT, MTT ET-50 tissue viability assay, MTT assay, Phototoxicity, Sun screens, UVA, UVB, Ultra-violet radiation (UV)
Results are reported using the human epidermis model (EpiDerm™) in a test protocol that was adopted from the method developed by ZEBET for phototoxicity testing with the model Skin2. In the new phototoxicity test the cytotoxicity of test materials applied topically to EpiDerm™ is determined at five concentrations in the absence and presence of a non-cytotoxic dose of UVA and visible light (sun simulation). Cytotoxicity is determined one day after irradiation in the MTT assay. Experiments performed to optimise test conditions are presented. Using appropriate phototoxic and non-phototoxic test chemicals our results demonstrate that a reconstructed human epidermal model, e.g. EpiDerm™, can be used in phototoxicity testing in the same way as a full skin model.
Reference Application
- Skin aging
- Intestinal barrier
- Liver Toxicity
- Consumer products
- Biocompatibility
- UV radiation
- Basic cutaneous research
- Epithelial restitution
- Microbial
- Pigmentation
- Transbuccal permeation/penetration
- Micronucleus Assay
- Skin disease
- SARS-CoV-2
- Skin Toxicity
- Cytotoxicity
- reproducibility
- Anti-aging
- Basic DC research
- Eye irritation
- Microbicide
- Pigmentation studies
- Oral mucosa
- microbiome
- Intestinal infection
- Skin re-epithelization
- Crohn's Disease
- Inflammatory response
- respiratory irritation
- Skin hydration
- UV
- Genetic toxicology
- Microbicide testing
- Radiation
- Tumor invasion
- Probiotic
- Skin differentiation
- Barrier repair
- Inflamed Bowel Disease
- Visible Light
- pesticides
- UV toxicity
- Basic respiratory research
- Genomics
- STD infection
- Reproducibility - eye (ocular) tissue model
- UV light
- Irritation>Eye Irritation OECD TG 492
- UV damage
- transporters
- Regulatory Approval
- Review Article
- LOAEL
- Absorption
- Antimicrobial
- Buccal delivery
- Genotoxicity
- Mucosal delivery
- Reproducibility - skin tissue models
- UV protection
- Corneal Drug Delivery
- Bacterial infection
- Pollution
- Mildness Testing
- Barrier Disruption
- UVB
- Drug delivery
- Infectious disease research
- Buccal drug delivery
- Pharmacotoxicology
- Nasal absorption
- Nanotechnology
- Aging
- Respiratory Disease
- DNA Damage
- Colitis
- Drug ADME
- Hair Growth
- scalp health
- Irritation
- Infection
- Mucosal irritation
- Psoriasis
- Vaginal irritation
- Respiratory immunotoxicity
- Human-on-a-chip
- Atopic Dermatitis
- Antiviral
- Gastrointestinal Disease
- Hazard assessment
- Biomedical Devices
- artificial saliva
- Permeation
- Infections
- Cosmetics
- Metabolism
- Mucous
- Respiratory infection
- Viral Infection
- Melanogenesis
- Bacterial colonization
- Translational toxicology
- Dry skin
- drug skin compatibility
- bacterial adherence
- Skin irritation
- Toxicity
- Cytokine analysis
- Immulogical research
- Nanoparticle toxicology/penetration
- Respiratory toxicology
- MMPs
- Intestinal Permeation
- Transbuccal drug delivery
- XtraMild skin mildness testing
- Skin moisturization
- Protein Expression
- PBPK Modeling
- Allergenicity
- Antioxidants
- Drug absorption
- Immunologicaal research
- Toxicology
- Skin cancer
- Photoaging
- Electrolyzed Water
- Oral Pathology
- bacterial vaginosis
- ADME
- Gastrointestinal Inflammation
- anti-wrinkling
- Immunological Research
- Apoptosis
- Intestinal toxicity
- Microbicides
- Nanotoxicology
- Skin corrosion
- Skin Sensitization
- Fibrosis
- Oral infection
- vaginal microbiome
- Microphysiological system
- Gastrointestinal Irritation
- Nicotine pouch products
- Immunogenicity
- Basic dermal research
- Respiratory research
- Immunotoxicity
- Ocular irritation
- Penetration
- Medical Devices
- Pulmonary Fibrosis
- Oral inflammation
- Collagen Remodeling
- Hyperpigmentation
- Barrier Function
- intranasal drug delivery
- Inflammation
- Asthma
- Skin corrosion Absorption
- Mucosal
- Oral candidiasis
- Skin lightening
- Organ-on-a-Chip
- Oxidative Stress
- Ocular toxicology
- Drug Screening
- Skin de-pigmentation
- Gastrointestinal Toxicity
- permeation enhancement
- Microbial infection
- COPD
- Smoking
- Respiratory toxicity
- Oral irritation
- Skin
- Oral Disease Research
- Skin Damage
- Validation
- Nephrotoxicity
- Cancer Research
- Skin Brightening
- excipients
- Wound healing
- Smoke
- Tobacco
- Inhalation Toxicology
- Oral mucositis
- Smoker
- Space Research
- Skin Barrier
- Biofilm
- Hepatotoxicity
- Personalized Medicine
- Food Additives
- Sub-categorization
- Phototoxicity
- Research
- Drug development
- Nanoparticles
- Skin pigmentation
- Gingivitis
- Dry Eye
- Drug Metabolism
Reference Product
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