EVALUATION OF XENOBIOTIC METABOLISM IN HUMAN RECONSTRUCTED SKIN MODELS FOR GENOTOXICITY TESTING.
- TR Number: 658
- Authors: Jackh1, C, Blatz1, V., Fabian1, E., Reisinger2 , K., van Ravenzwaay1 , B., Trappe3, S., Landsiedel1, R. 1Experimental Toxicology and Ecology, BASF SE, 67056 Ludwigshafen, Germany. 2Henkel AG & CO. KGaA, 40191 Düsseldorf, Germany. 3Federal Institute for Risk Assessment (BfR), 14195 Berlin, Germany.
Skin penetration and absorption is a major route of xenobiotic uptake. Skin maintains furthermore capacities to transform chemical substances which was previously shown by transcriptional expression analysis of metabolizing enzymes including CYPs, Flavin-dependent monooxygenases (FMO), N- Acetyltransferases (NAT) and UDP-Glucuronyltransferases (UDP-GT) [1-3]. Their catalytic activity in skin tissue however was rarely specified. Human reconstructed skin models are under investigation to be used as in vitro alternative for in vivo animal testing. As metabolic processing is required for chemical induced effects, i.e. genotoxicity, characterization of enzymatic competence of in vitro skin models is demanded for their usage in toxicity testing.
Reference Application
- UV radiation
- Basic cutaneous research
- Epithelial restitution
- Microbial
- Pigmentation
- Transbuccal permeation/penetration
- Micronucleus Assay
- Skin aging
- Intestinal barrier
- Liver Toxicity
- Consumer products
- Anti-aging
- Basic DC research
- Eye irritation
- Microbicide
- Pigmentation studies
- Oral mucosa
- microbiome
- Skin disease
- SARS-CoV-2
- Skin Toxicity
- Cytotoxicity
- Skin hydration
- UV
- Genetic toxicology
- Microbicide testing
- Radiation
- Tumor invasion
- Probiotic
- Intestinal infection
- Skin re-epithelization
- Crohn's Disease
- Inflammatory response
- UV toxicity
- Basic respiratory research
- Genomics
- STD infection
- Reproducibility - eye (ocular) tissue model
- UV light
- Irritation>Eye Irritation OECD TG 492
- Skin differentiation
- Barrier repair
- Inflamed Bowel Disease
- Visible Light
- Absorption
- Antimicrobial
- Buccal delivery
- Genotoxicity
- Mucosal delivery
- Reproducibility - skin tissue models
- UV protection
- Corneal Drug Delivery
- UV damage
- transporters
- Regulatory Approval
- Review Article
- Drug delivery
- Infectious disease research
- Buccal drug delivery
- Pharmacotoxicology
- Nasal absorption
- Nanotechnology
- Aging
- Respiratory Disease
- Bacterial infection
- Pollution
- Mildness Testing
- Barrier Disruption
- Irritation
- Infection
- Mucosal irritation
- Psoriasis
- Vaginal irritation
- Respiratory immunotoxicity
- Human-on-a-chip
- Atopic Dermatitis
- DNA Damage
- Colitis
- Drug ADME
- Hair Growth
- Permeation
- Infections
- Cosmetics
- Metabolism
- Mucous
- Respiratory infection
- Viral Infection
- Melanogenesis
- Antiviral
- Gastrointestinal Disease
- Hazard assessment
- Biomedical Devices
- Skin irritation
- Toxicity
- Cytokine analysis
- Immulogical research
- Nanoparticle toxicology/penetration
- Respiratory toxicology
- MMPs
- Intestinal Permeation
- Bacterial colonization
- Translational toxicology
- Dry skin
- drug skin compatibility
- Allergenicity
- Antioxidants
- Drug absorption
- Immunologicaal research
- Toxicology
- Skin cancer
- Photoaging
- Electrolyzed Water
- Transbuccal drug delivery
- XtraMild skin mildness testing
- Skin moisturization
- Protein Expression
- Immunological Research
- Apoptosis
- Intestinal toxicity
- Microbicides
- Nanotoxicology
- Skin corrosion
- Skin Sensitization
- Fibrosis
- Oral Pathology
- bacterial vaginosis
- ADME
- Gastrointestinal Inflammation
- Immunogenicity
- Basic dermal research
- Respiratory research
- Immunotoxicity
- Ocular irritation
- Penetration
- Medical Devices
- Pulmonary Fibrosis
- Oral infection
- vaginal microbiome
- Microphysiological system
- Gastrointestinal Irritation
- Inflammation
- Asthma
- Skin corrosion Absorption
- Mucosal
- Oral candidiasis
- Skin lightening
- Organ-on-a-Chip
- Oxidative Stress
- Oral inflammation
- Collagen Remodeling
- Hyperpigmentation
- Barrier Function
- Microbial infection
- COPD
- Smoking
- Respiratory toxicity
- Oral irritation
- Skin
- Oral Disease Research
- Skin Damage
- Ocular toxicology
- Drug Screening
- Skin de-pigmentation
- Gastrointestinal Toxicity
- Wound healing
- Smoke
- Tobacco
- Inhalation Toxicology
- Oral mucositis
- Smoker
- Space Research
- Skin Barrier
- Validation
- Nephrotoxicity
- Cancer Research
- Skin Brightening
- Phototoxicity
- Research
- Drug development
- Nanoparticles
- Skin pigmentation
- Gingivitis
- Dry Eye
- Drug Metabolism
- Biofilm
- Hepatotoxicity
- Personalized Medicine
- Food Additives
Reference Product
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