SIMULTANEOUS DETECTION AND SEMIQUANTIFICATION OF DNA DAMAGE IN NORMAL AND APOPTOTIC CELLS: TRIPLE-IMMUNOFLUORESCENT LABELING USING DAPI, ANTIBODIES, AND TUNEL.
We developed a triple-labeling immunofluorescence technique that simultaneously identifies total DNA (DAPI), DNA damage (antibodies), and dead cells [terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL)-positive cells] and a method that semiquantifies DNA damage in paraffin-embedded tissues. Using this technique in combination with our analysis method, scientists can now simultaneously detect and compare the relative amounts […]