Skip to Main Content
+1 (508) 881-6771
0
Menu Button

EpiGingival

1034

Toxicological Aspects of in vitro Oral Mucosa Wound Healing Models

Wounds in the oral cavity tissues can result from surgical procedures, accidents, and canker sores. Oral wounds present a site for potential infection and increased sensitivity to oral irritants. Untreated wounds can lead to pathogen invasion, chronic pain, poor cosmetic outcomes, and in some cases, extended hospitalization. Thus, damaged oral mucosal tissue needs to be […]
934

Low-temperature plasma on peri-implant-related biofilm and gingival tissue

Background: Evaluate the effect of low-temperature plasma (LTP) on an anaerobic biofilm and on the biological response of an in vitro reconstituted gingival epithelium tissue. Methods: Porphyromonas gingivalis W83 biofilm was cultured on titanium discs and reconstituted gingival tissues were submitted to similar treatment conditions. Treatments: LTP1—plasma treatment for 1 minute, LTP3—plasma treatment for 3 […]
919

In vitro three-dimensional organotypic culture models of the oral mucosa

Three-dimensional, organotypic models of the oral mucosa have been developed to study a wide variety of phenomena occurring in the oral cavity. Although a number of models have been developed in academic research labs, only a few models have been commercialized. Models from academic groups offer a broader range of phenotypes while the commercial models […]
909

The expression of gingival epithelial junctions in response to subgingival biofilms

Periodontitis is an infectious inflammatory disease that destroys the tooth-supporting tissues. It is caused by the formation of subgingival biofilms on the surface of the tooth. Characteristic bacteria associated with subgingival biofilms are the Gram-negative anaerobes Porphyromonas gingivalis, Tannerella forsythia and Treponema denticola, collectively known as the “red complex” species. Inter-epithelial junctions ensure the barrier […]
905

Potassium is a key signal in host-microbiome dysbiosis in periodontitis

Dysbiosis, or the imbalance in the structural and/or functional properties of the microbiome, is at the origin of important infectious inflammatory diseases such as inflammatory bowel disease (IBD) and periodontal disease. Periodontitis is a polymicrobial inflammatory disease that affects a large proportion of the world’s population and has been associated with a wide variety of […]
896

Endogenous acid ceramidase protects epithelial cells from Porphyromonas gingivalis-induced inflammation in vitro

Ceramidases are a group of enzymes that degrade pro-inflammatory ceramide by cleaving a fatty acid to form anti-inflammatory sphingosine lipid. Thus far, acid, neutral and alkaline ceramidase isozymes have been described. However, the expression patterns of ceramidase isoforms as well as their role in periodontal disease pathogenesis remain unknown. In this study, expression patterns of […]
879

Development of a Model for Assessment of Blue Light Activated Toxicity in EpiGingival™ and EpiGingival™-FT Tissues

Traditional phototoxicity models examine activation of test material by ultraviolet A (UVA) light. In these models, the ability of a test chemical to induce toxicity in cells or tissues after light exposure is compared to non-light exposed tissues. While UV-induced toxicity is of great concern, it is clear that lower energy light in the blue […]
870

E-cigarettes and flavorings induce inflammatory and prosenescence responses in oral epithelial cells and periodontal fibroblasts

Electronic-cigarettes (e-cigs) represent a significant and increasing proportion of tobacco product consumption, which may pose an oral health concern. Oxidative/carbonyl stress via protein carbonylation is an important factor in causing inflammation and DNA damage. This results in stress-induced premature senescence (a state of irreversible growth arrest which re-enforces chronic inflammation) in gingival epithelium, which may […]
859

Novel antioxidants are not toxic to normal tissues but effectively kill cancer cells

Free radicals are formed as a result of cellular processes and play a key role in predisposition to and development of numerous diseases and of premature aging. Recently, we reported the syntheses of a number of novel phenolic antioxidants for possible application in food industry. In the present study, analyses of the cellular processes and […]
856

A series of in vitro and human studies of a novel lip cream formulation for protecting against environmental triggers of recurrent herpes labialis

Purpose: These studies describe the testing of a novel, daily-use lip cream designed for individuals with lips prone to recurrent herpes labialis (RHL) that protects against environmental triggers. Subjects and methods: In vitro occlusive and in vitro and in vivo photoprotection analyses, a characterization of normal vs dry lips, and a randomized, evaluator-blinded, clinical trial […]
855

Behaviour of Silver Nanoparticles in Simulated Saliva and Gastrointestinal Fluids

Continuously increasing application of silver nanoparticles (AgNPs) requires information on their safety and performance under biological conditions. Assessment of AgNPs in biological systems is also related to availability of robust toxicological methods for evaluation of toxic potential of AgNPs and information on their physicochemical state. Silver nanoparticles were subjected to action of simulated saliva, gastric […]
799

In vitro systems toxicology approach to investigate the effects of repeated cigarette smoke exposure on human buccal and gingival organotypic epithelial tissue cultures

Smoking has been associated with diseases of the lung, pulmonary airways and oral cavity. Cytologic, genomic and transcriptomic changes in oral mucosa correlate with oral preneoplasia, cancer and inflammation (e.g. periodontitis). Alteration of smoking-related gene expression changes in oral epithelial cells is similar to that in bronchial and nasal epithelial cells. Using a systems toxicology approach, we have previously […]
715

NUCLEOTIDE EXCISION REPAIR IS REDUCED IN ORAL EPITHELIAL TISSUES COMPARED WITH SKIN.

Ultraviolet radiation (UVR) exposure to internal tissues for diagnostic, therapeutic and cosmetic procedures has increased dramatically over the past decade. The greatest increase in UVR exposure of internal tissues occurs in the cosmetic industry where it is combined with oxidizing agents for teeth whitening, often in conjunction with indoor tanning. To address potential carcinogenic risks […]
672

RETENTION OF O-CYMEN-5-OL AND ZINC ON RECONSTRUCTED HUMAN GINGIVAL TISSUE FROM A TOOTHPASTE FORMULATION.

Objectives:  To assess the retention of o-cymen-5-ol and zinc on reconstructed human gingival tissue delivered by topical applications of toothpaste formulated with 0.1%w⁄w o-cymen-5-ol and 0.6%w⁄w zinc chloride (ZnCl2). Methods:  EpiGingivalTM tissues were treated topically for 2 minutes with either solutions or toothpaste slurries containing o-cymen-5-ol and ZnCl2. Tissues were rinsed with water between application […]
450

METHODS FOR ASSESSMENT OF PROLIFERATION IN AN IN VITRO TISSUE MODEL OF ORAL EPITHELIA.

This study by scientists at the University of Colorado and Premier Laboratory, LLC demonstrated that a robust method for the assessment of the proliferative effects of agents applied to Mattek’s EpiOral and EpiGingival in vitro tissue equivalents includes measurements of epithelial thickness, basal cell layer thickness, number of cells in the basal cell layer, and […]
773

Effects of ozone nano-bubble water on periodontopathic bacteria and oral cells – in vitro studies

The aims of the present study were to evaluate the bactericidal activity of a new antiseptic agent, ozone nano-bubble water (NBW3), against periodontopathogenic bacteria and to assess the cytotoxicity of NBW3 against human oral cells. The bactericidal activities of NBW3 against representative periodontopathogenic bacteria, Porphyromonas gingivalis (P. gingivalis) and Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) were evaluated […]
263

GINGIVAL TISSUE MODEL FOR PREDICTION OF IRRITANCY OF ORAL CARE PRODUCTS.

The development of human reconstructed tissues of the oral cavity that express in vivo-like differentiated characteristics would be important to better understand and develop therapies for gum disease, oral cancer, lesions due to smokeless tobacco, and gingivitis, amongst other diseases of the oral mucosa. In vitro tissue models would be used to assess the biocompatibility […]
683

UPDATE ON VALIDATION STATUS AND INDUSTRY UTILIZATION OF NORMAL HUMAN 3D (NHU-3D) ANIMAL ALTERNATIVE MODELS.

Progress in in vitro tissue engineering over the last several decades has contributed to a significant decrease in animal use for industrial risk and safety assessment of products and ingredients. Mattek Corporation has a long-standing involvement in development, production and validation of animal alternative in vitro models produced from normal human cells (NHu-3D models). The […]
399

CHARACTERIZATION AND TESTING OF NEW BUCCAL AND GINGIVAL TISSUE MODELS.

This study by scientists at the University of Washington, University of Iowa and Mattek Corp. demonstrated that Mattek’s EpiOral and EpiGingival in vitro human buccal and gingival oral tissue equivalents showed good correspondence to human explant buccal and gingival tissue structure, protein expression, and lipid content; reproducibility of the tissues was very good; and differences […]
379

EPIGINGIVAL™ (GIN-100) TISSUE MODELS FOR ORAL IRRITATION STUDIES.

This study by scientists at the University of Washington, University of Iowa and Mattek Corp. demonstrated that Mattek’s EpiOral and EpiGingival in vitro human oral tissue equivalents can be used to provide a quick, reproducible method for evaluating the irritation potential of oral care excipients and oral care products, that the methodology correlates well to […]
757

ORGANOTYPIC HUMAN ORAL TISSUE MODELS FOR EVALUATION OF ORAL CARE PRODUCTS.

Oral irritation can result from exposure of the soft tissue to dentifrice and oral rinse formulations. In vitro tissue models (Mattek Corporation) produced from normal human oral keratinocytes were exposed to common oral care excipients, dentifrice supernatants and mouth rinses to determine the potential irritation of oral care excipients/formulations, including SLS, poloxamer, NaF dentifrice, and […]
439

INFECTION OF HUMAN CYTOMEGALOVIRUS IN CULTURED HUMAN GINGIVAL TISSUE.

This study by researchers at UC Berkeley School of Public Health demonstrated that Mattek’s EpiGingival in vitro human tissue equivalent can be used to study human cytomegalovirus (HCMV) infection, and to screen antivirals that block viral replication and transmission in the oral cavity. Background: Human cytomegalovirus (HCMV) infection in the oral cavity plays an important […]
438

ORGANOTYPIC HUMAN ORAL TISSUE MODELS FOR TOXICOLOGICAL STUDIES.

This study by researchers at Procter & Gamble Co., the University of Iowa and Mattek Corp. demonstrated that Mattek’s EpiOral and EpiGingival human oral cavity tissue equivalents can be used to screen the irritation potential of newly developed oral care products and will be useful to study the innate immunity, biology and pathology of the […]