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EpiDermFT

1054

Hydrophobic binary mixtures containing amphotericin B as lipophilic solutions for the treatment of cutaneous leishmaniasis

Cutaneous leishmaniasis, caused by Leishmania parasites, requires treatments with fewer side effects than those currently available. The development of a topical solution based on amphotericin B (AmB) was pursued. The considerable interest in deep eutectic solvents (DESs) and their remarkable advantages inspired the search for a suitable hydrophobic excipient. Various mixtures based on commonly used […]
1052

Three-Dimensional Cultured Human Dermal Papilla Cells in HGC-Coated Environments Enhance Hair Regeneration and Artificial Skin Integration

The rapid development of biomimetic materials in the field of regenerative medicine has made it possible to mimic natural cellular environments and allow in vitro systems to replace in vivo systems. In particular, the emergence of hexanoyl glycol chitosan (HGC) is playing an important role the development of 3-dimensional (3D) cell culture and tissue engineering. […]
1051

Spectral and mass characterization of kinetic conversion from retinoids to retinoic acid in an in vitro 3-D human skin equivalent model

To investigate the effect of retinoids, such as retinol (ROL), retinal (RAL), and retinyl palmitate (RP), on epidermal integrity, skin deposition, and bioconversion to retinoic acid (RA). 3-D human skin equivalent model (EpiDermFT™) was used. Epidermal cellular integrity measured by TEER values was significantly higher for a topical treatment of ROL and RAL than RP […]
1044

Ionic liquids as a class of materials for transdermal delivery and pathogen neutralization

Biofilm-protected microbial infections in skin are a serious health risk that remains to be adequately addressed. The lack of progress in developing effective treatment strategies is largely due to the transport barriers posed by the stratum corneum of the skin and the biofilm. In this work, we report on the use of Ionic Liquids (ILs) […]
1027

Kinetic conversion of BIOGF1K enriched in compound K from in vitro 3-D human tissue model

The purposes of current study were to investigate the effect of ginsenosides from BIOGF1K enriched in compound K (CK) and compound Y (CY) on the skin barrier function, the deposition in in vitro 3-D human tissue model (EpiDermFT™ Full Thickness 400), and to identify and quantify kinetic bioconversion of the ginsenosides in artificial skin by utilizing the […]
1011

Targeting Multiple Hallmarks of Skin Aging: Preclinical and Clinical Efficacy of a Novel Growth Factor-Based Skin Care Serum

The aging process involves numerous biological mechanisms that have been characterized and proposed as the ‘‘hallmarks of aging.’’ Targeting the processes and pathways related to these hallmarks of aging that cause and promote skin aging could provide anti-aging benefits. A novel topical growth factor-based skin care serum (A+) was developed using human fibroblast conditioned media. […]
1010

Efficacy and Tolerability of a Novel Topical Treatment for the Neck: A Randomized, Double-Blind Regimen-Controlled Study

The neck plays a telling role as an age indicator. Due to its anatomy and function, neck skin ages differently than facial skin and special considerations need to be taken when providing treatment. A randomized, double-blind, regimen-controlled study was conducted to assess the efficacy and tolerability of a novel topical cosmetic cream (NCC) specifically tailored […]
985

Anti-Glycation and Anti-Aging Properties of Resveratrol Derivatives in the in-vitro 3D Models of Human Skin

Purpose: Human skin undergoes modifications affecting its structural properties and barrier functions involved in protection against age-related damage. Glycation is a non-enzymatic reaction between macromolecules and sugars causing alterations to the elastic fibers and premature aging of the skin. Glycation can be prevented by a range of bioactive molecules; however, at present only a few […]
981

The soybean cultivar SCEL‑1 shows potent anti‑photoaging effects in a UV‑induced three‑dimensional human skin and hairless mouse model

Soybean (Glycine max) is one of the major sources of nutrition and is used as a raw material for food and as a source of feed for livestock. The efficacy of soybeans on skin health includes their ability to reduce wrinkles and pigmentation and increase skin elasticity and moisture content. Black soybean has been consumed […]
979

Regeneration of collagen fibrils at the papillary dermis by reconstructing basement membrane at the dermal–epidermal junction

The epidermal basement membrane deteriorates with aging. We previously reported that basementmembrane reconstruction not only serves to maintain epidermal stem/progenitor cells in the epidermis, but also increases collagen fibrils in the papillary dermis. Here, we investigated the mechanism of the latter action. Collagen fibrils in the papillary dermis were increased in organotypic human skin culture […]
977

Anti-aging Effects of Retinoid Hydroxypinacolone Retinoate on Skin Models

Tretinoin, also known as all-trans retinoic acid (ATRA), has been well-known for its anti-aging effects on skin since the 1980s.1,2 ATRA functions by activating the nuclear retinoic acid family of receptors (RARs), which control gene transcription by binding to retinoic acid responsive elements (RAREs) in DNA.3 Retinoids are thought to reduce wrinkles by increasing type […]
976

2-kDa hyaluronan ameliorates human facial wrinkles through increased dermal collagen density related to promotion of collagen remodeling

Background/Aims: Hyaluronan (HA) oligosaccharides are involved in several biological processes, primarily collagen remodeling and wound healing. Collagen remodeling is retarded in aging skin and causes wrinkles. The aim of this study was to evaluate the effect of 2-kDa HA oligosaccharides (HA2k) on wrinkles by permeation through the stratum corneum and promotion of collagen remodeling. Methods: […]
975

Transcriptomic Analysis of Healthy and Atopic Dermatitis Samples Reveals the Role of IL-37 in Human Skin

Atopic dermatitis (AD) is a chronic inflammatory skin disease that affects up to one in five children and millions of adults in developed countries. Clinically, AD skin lesions manifest as subacute and/or chronic lichenified eczematous plaques, which are often intensely pruritic and prone to secondary bacterial and viral infections. Despite the emergence of novel therapeutic […]
974

A Validated Method for Assessing the Effects of Blue Light on the Human Skin

 The COVID pandemic caused an increase in virtual meetings and work from home scenarios that resulted in people spending increased time indoors and in front of computer screens and electronic devices. It is estimated that 60% of people spend more than six hours per day in front of a digital device. Studies have shown that […]
961

Spermidine-induced recovery of human dermal structure and barrier function by skin microbiome

An unbalanced microbial ecosystem on the human skin is closely related to skin diseases and has been associated with inflammation and immune responses. However, little is known about the role of the skin microbiome on skin aging. Here, we report that the Streptococcus species improved the skin structure and barrier function, thereby contributing to anti-aging. […]
959

Immunomodulation of Skin Cytokine Secretion by House Dust Mite Extracts

Background: Skin contact with house dust mites may contribute to atopic dermatitis and other skin diseases. We sought to determine if molecules from house dust mites could influence the release of proinflammatory cytokines and chemokines from epidermal keratinocytes and dermal fibroblasts grown in a human skin equivalent (HSE) model. Methods: HSEs consisting of an epidermis […]
957

In vitro and in vivo evaluation of a moisture treatment cream containing three critical elements of natural skin moisturization

Objectives: To evaluate skin barrier and hydration effects of a new rebalancing moisture treatment (TRMT) and to assess efficacy and tolerability in subjects with photodamaged skin. Methods: In an epidermal skin model, tissues (n = 5/group) were topically treated with 25 μL of TRMT, 25 μL of a market-leading moisturizer (MLM), or untreated for 60 […]
940

Synthetic Retinoid Seletinoid G Improves Skin Barrier Function through Wound Healing and Collagen Realignment in Human Skin Equivalents

The outer epidermal skin is a primary barrier that protects the body from extrinsic factors, such as ultraviolet (UV) radiation, chemicals and pollutants. The complete epithelialization of a wound by keratinocytes is essential for restoring the barrier function of the skin. However, age-related alterations predispose the elderly to impaired wound healing. Therefore, wound-healing efficacy could […]
933

A mixed community of skin microbiome representatives influences cutaneous processes more than individual members

Skin, the largest organ of the human body by weight, hosts a diversity of microorganisms that can influence health. The microbial residents of the skin are now appreciated for their roles in host immune interactions, wound healing, colonization resistance, and various skin disorders. Still, much remains to be discovered in terms of the host pathways […]
931

Demonstration of the first-pass metabolism in the skin of the hair dye, 4-amino-2-hydroxytoluene, using the Chip2 skin–liver microphysiological model

We used TissUse’s HUMIMIC Chip2 microfluidic model, incorporating reconstructed skin models and liver spheroids, to investigate the impact of consumer-relevant application scenarios on the metabolic fate of the hair dye, 4-amino-2-hydroxytoluene (AHT). After a single topical or systemic application of AHT to Chip2 models, medium was analysed for parent and metabolites over 5 days. The […]
930

Microvesicating effects of sulfur mustard on an in vitro human skin model

Bis-(b-chloroethyl) sulfide (SM) is a potent skin vesicant previously used for chemical warfare. Progress in determination of the mechanistic basis of SM pathology, and development of prophylactic and/or therapeutic countermeasures to SM exposure has been hampered by lack of physiologically relevant models of human skin. The current work evaluated a newly developed tissue engineered full-thickness […]
917

Microbially competent 3D skin: a test system that reveals insight into host–microbe interactions and their potential toxicological impact

The skin`s microbiome is predominantly commensalic, harboring a metabolic potential far exceeding that of its host. While there is clear evidence that bacteria-dependent metabolism of pollutants modulates the toxicity for the host there is still a lack of models for investigating causality of microbiome-associated pathophysiology or toxicity. We now report on a biologically characterized microbial–skin […]
916

Evaluation of Inflammation by Cytokine Production Following Combined Exposure to Ultraviolet and Radiofrequency Radiation of Mobile Phones on 3D Reconstructed Human Skin In Vitro

The absorption of exposure to radiofrequency (RF) emitted by wireless devices leads to a high specific absorption rate in the skin. Ultraviolet (UV) radiation can induce several damages to the skin. The aim of this study was to examine whether combined, consecutive exposure to solar UV radiation and 1950 MHz RF exposure of third generation […]
895

Intense THz pulses cause H2AX phosphorylation and activate DNA damage response in human skin tissue

Recent emergence and growing use of terahertz (11-Iz) radiation for medical imaging and public security screening raise questions on reasonable levels of exposure and health consequences of this form of electromagnetic radiation. In particular, picosecond-duration 11-Iz pulses have shown promise for novel diagnostic imaging techniques. However, the effects of THz pulses on human cells and […]
894

Anti-Aging Potentials of Methylene Blue for Human Skin Longevity

Oxidative stress is the major cause of skin aging that includes wrinkles, pigmentation, and weakened wound healing ability. Application of antioxidants in skin care is well accepted as an effective approach to delay the skin aging process. Methylene blue (MB), a traditional mitochondrial-targeting antioxidant, showed a potent ROS scavenging efficacy in cultured human skin fibroblasts […]
893

Nell1 Protein Reduces the Number of Sunburned Cells and Modulates the Inflammatory Response after UV-induced Damage in a 3D Human Skin Model

NELL1 is a novel signaling protein that mediates tissue growth and maturation during fetal development and stimulates tissue healing post-injury in adult tissues. NELL1 promotes the production of a regenerative (fetal type) extracellular matrix (ECM) that provides the structural and communication framework for several processes essential to tissue repair and regeneration such as cell proliferation, […]
856

A series of in vitro and human studies of a novel lip cream formulation for protecting against environmental triggers of recurrent herpes labialis

Purpose: These studies describe the testing of a novel, daily-use lip cream designed for individuals with lips prone to recurrent herpes labialis (RHL) that protects against environmental triggers. Subjects and methods: In vitro occlusive and in vitro and in vivo photoprotection analyses, a characterization of normal vs dry lips, and a randomized, evaluator-blinded, clinical trial […]
849

Induction of an Atopic Dermatitis-Like Phenotype in a Full-Thickness in vitro Human Skin Model

Atopic dermatitis (AD) is the most common inflammatory disease of the skin and is characterized by defects in keratinocyte differentiation and skin barrier dysfunction. The goal of the current work is to create an in vitro AD model for use in basic research and therapeutic development. To create the model, the in vitro full-thickness human […]
848

Hypoxia leads to abnormal epidermal differentiation via HIF-independent pathways

Atmospheric oxygen is important for the epidermis, as the skin epidermis is not greatly affected by blood circulation. Therefore, it is necessary to understand the effect of hypoxic signals on the epidermis as some environmental stimuli can induce skin hypoxia. Here, we investigated how hypoxia (1% O2) affected skin equivalents (SEs) and normal human epidermal […]
847

A Novel Assay for Evaluating Wound Healing in a Full-Thickness in vitro Human Skin Model

Wound healing is a fundamental process to re-establish tissue integrity and skin barrier function. A model of wound healing was created by introducing epidermal wounds in a full thickness in vitro human skin model (EpiDermFT) using a 3-mm punch biopsy and subsequently evaluated at multiple recovery time points. EpiDermFT exhibits stratified epidermal components and a […]
809

Application of Mattek In Vitro Reconstructed Human Skin Models for Safety, Efficacy Screening, and Basic Preclinical Research

During the past three decades, great progress has been achieved in the development of in vitro reconstructed human epidermal (RhE) tissue models. This technology is now available via various manufacturers of commercially available tissue models, and has provided opportunities for researchers worldwide to reduce or even completely avoid in vivo experimentation for screening and prediction of cutaneous […]
802

Combined reflectance confocal microscopy/optical coherence tomography imaging for skin burn assessment

A combined high-resolution reflectance confocal microscopy (RCM)/optical coherence tomography (OCT) instrument for assessing skin burn gravity has been built and tested. This instrument allows for visualizing skin intracellular details with submicron resolution in the RCM mode and morphological and birefringence modifications to depths on the order of 1.2 mm in the OCT mode. Preliminary testing of […]
795

Total Defense + Repair: A Novel Concept in Solar Protection and Skin Rejuvenation

For more than a century, solar radiation has been known to contribute Significantly to the extrinsic aging of skin. Until recently, this was almost exclusively attributed to the photodamage caused by ultraviolet (UV) light. However, a growing body of evidence now indicates that both infrared (IR) and visible light may also contribute to extrinsic skin aging. Infrared […]
651

TIMECOURSE OF HEALING AFTER UV-INDUCED DAMAGE IN THE EPIDERM-FT IN VITRO HUMAN SKIN MODEL.

Solar ultraviolet (UV) radiation is known to have deleterious effects on human skin.  UVB (280-320 nm) and UVA (320-400 nm) segments of solar radiation have been shown to affect keratinocytes, the major cellular constituent of the epidermis, by causing direct DNA damage and/or indirect DNA damage and cytotoxicity through the formation of reactive oxygen species […]
627

IMPROVED HEALING IN TISSUE CULTURE MODEL OF VESICANT INJURY SHOWN BY HIGH FEATURE ANTIMICROBIAL DRESSING.

Quick-Med Technologies (QMT) has developed a dressing for the treatment of vesicant injuries following debridement under an army Small Business Innovation Research (SBIR) program grant. This dressing is designed to enable optimal wound healing by providing a moist wound healing environment with antimicrobial protection and protease inhibiting properties, through sustained delivery of an antibiotic (doxycycline) […]
610

CUTANEOUS WOUND HEALING IN THE EPIDERMFT™ FULL-THICKNESS IN VITRO HUMAN SKIN MODEL: ROLE OF SERUM GROWTH FACTORS.

Timely and efficient epidermal wound healing is necessary for normal skin barrier function.  Cutaneous wound healing involves interactions between dermal fibroblasts and epidermal keratinocytes as well as cell-extracellular matrix interactions (1-4).  The current study describes wound healing experiments conducted in a full-thickness in vitro human skin model (EpiDerm-FT).  This model exhibits stratified epidermal components and […]
527

TO DEVELOP AN IN-VITRO (EFT-300) SKIN MODEL FOR THE STUDY OF WOUND HEALING.

This study by scientists at Florida A&M University College of Pharmacy and Pharmaceutical Sciences and Mattek Corp. demonstrated that Mattek’s EpiDermFT full-thickness in vitro human skin tissue equivalent is an excellent in vitro model for studying the human skin wound healing process. Wound healing is a natural process, which involves the regeneration of epidermal and […]
485

HEALING OF DERMAL WOUNDS IN THE EPIDERM-FT™ IN VITRO HUMAN SKIN MODEL: AN ANIMAL ALTERNATIVE FOR WOUND HEALING ISSUES.

This study by scientists at Mattek Corp. demonstrated that Mattek’s EpiDerm-FT full thickness in vitro human skin tissue equivalent is an excellent in vitro model for studying the dermal-epidermal wound-healing interactions produced by physical wounding of these tissues. Background: Dermal wound healing involves interactions between dermal fibroblasts and epidermal keratinocytes, as well as cell and […]
406

MATRIX METALLOPROTEINASE (MMP) EXPRESSION IN THE EPIDERM-FT SKIN EQUIVALENT: RELEVANCE TO DERMAL WOUND HEALING AND BLISTERING SKIN DISEASES.

Using Mattek’s EpiDermFT full-thickness in vitro human skin equivalent, scientists at Mattek Corp. showed that epidermal keratinocytes have a significant influence over the expression of matrix metalloproteinases (MMP’s) by dermal fibroblasts, demonstrating why EpiDermFT is useful in investigating MMP expression and how it affects dermal wound healing, tissue remodeling, blistering diseases, tumor invasion and metastasis. […]
404

TOPICALLY APPLIED LIPOSOME-ENCAPSULATED T4 ENDONUCLEASE V INCREASES REPAIR OF UV-INDUCED CYCLOBUTANE PYRIMIDINE DIMERS IN NORMAL REPAIR PROFICIENT RECONSTRUCTED SKIN.

This study by scientists at AGI Dermatics demonstrated that Mattek’s EpiDermFT full-thickness in vitro human skin tissue equivalent can be used as a model for DNA repair in human skin and to investigate the effects of topical application of liposome-encapsulated DNA repair enzymes. Reconstructed skin is a useful model because it uses human cells and […]
362

THE EFFECT OF SCARGUARD ON COLLAGENASE LEVELS USING A FULL-THICKNESS EPIDERMAL MODEL.

This study by scientists at Chondrex, Inc. and the Albert Einstein School of Medicine demonstrated that Mattek’s EpiDermFT full-thickness in vitro human skin tissue equivalent can be used to assess the ability of formulations to affect the release of procollagenase, an essential mediator in the (human skin) wound healing process. Background: Despite meticulous wound care […]
323

WOUND HEALING RESPONSE OF THE EPIDERM FULL THICKNESS (EPIDERM-FT™) IN VITRO HUMAN SKIN EQUIVALENT AFTER SOLAR UV IRRADIATION: COMPARISON TO EXCISED HUMAN SKIN.

Normal human epidermal keratinocytes (KC) and dermal fibroblasts (FB) were cultured to produce full-thickness skin equivalents consisting of FB-containing dermal and stratified epidermal components with a fully developed basement membrane at the dermal/epidermal junction (EpiDerm-FT 200, EFT). The wound healing response of EFT after solar UV-irradiation was compared to excised human skin. H&E stained paraffin […]
286

EPIDERM™ FULL THICKNESS (EPIDERM-FT™), A DERMAL-EPIDERMAL SKIN MODEL WITH A FULLY DEVELOPED BASEMENT MEMBRANE.

Paracrine signaling between dermal fibroblasts (FB) and epidermal keratinocytes (KC) is believed to modulate skin responses during contact irritant or allergic reactions. Dermal FB also play an important role in photo-aging and photo-damage, wound healing and cancer progression. To enable in vitro study of these and other dermal phenomena in which FB-KC interactions are important, […]
677

BACTERIAL INACTIVATION OF WOUND INFECTION IN A HUMAN SKIN MODEL BY LIQUID-PHASE DISCHARGE PLASMA.

Background:  We investigate disinfection of a reconstructed human skin model contaminated with biofilm-formative Staphylococcus aureus employing plasma discharge in liquid. Principal Findings: We observed statistically significant 3.83-log10 (p,0.001) and 1.59-log10 (p,0.05) decreases in colony forming units of adherent S. aureus bacteria and 24 h S. aureus biofilm culture with plasma treatment. Plasma treatment was associated […]
630

3D-WOUND HEALING MODEL: INFLUENCE OF MORPHINE AND SOLID LIPID NANOPARTICLES.

For efficient pain reduction in severe skin wounds, topically applied opioids may be a new option. Moreover, by stimulating keratinocyte migration opioids may also accelerate wound healing. Yet, conventional formulations failed to consistently provide sufficient pain control in patients which may be due to local drug  degradation or insufficient concentrations at the target site. After […]
293

EPIDERM™ FULL THICKNESS (EPIDERMFT™), A DERMAL/EPIDERMAL SKIN EQUIVALENT WITH A FULLY DEVELOPED BASEMENT MEMBRANE.

Paracrine signaling between dermal fibroblasts and epidermal keratinocytes is believed to modulate skin responses during contact irritant or allergic reactions. Dermal fibroblasats also play an important role in photo-aging and photo-damage, wound healing and cancer progression. To enable in vitro study of these and other dermal phenomena in which fibroblast-epidermal keratinocyte interactions are important, a […]
328

WOUND HEALING RESPONSE OF A FULL THICKNESS IN VITRO HUMAN SKIN EQUIVALENT (EPIDERM-FT™ 200) AFTER SOLAR UV-IRRADIATION.

Normal human epidermal keratinocytes (KC) and dermal fibroblasts (FB) were cultured to produce full-thickness skin equivalents with FB-containing dermal and stratified epidermal components and fully developed basement membrane at the dermal/epidermal junction (EpiDermFT 200). The wound healing response of EpiDermFT after solar UV-irradiation was compared to excised human skin. H&E stained paraffin sections of EpiDermFT […]
739

A THERMOSENSITIVE MORPHINE-CONTAINING HYDROGEL FOR THE TREATMENT OF LARGE-SCALE SKIN WOUNDS.

Purpose:  Topically applied opioids are an option to induce efficient analgesia in patients with severe skin wounds. For ongoing pain reduction, the vehicle should provide sustained drug release in order to increase the intervals during the regular wound dressing changes. In addition, the formulation should not impair wound healing. Hydrogels provide a moist wound environment, […]
652

USE OF EPIDERM FULL THICKNESS (EFT) SKIN CULTURES AS AN IN VITRO MODEL FOR WOUND HEALING.

Background:  Wound healing is a natural process which involves the regeneration of epidermal and dermal tissue with expression of growth factors and inflammatory markers. The objective of this study was to evaluate the EpiDerm full thickness (EpiDerm-FT, EFT) skin culture as an in-vitro wound healing model to understand the healing process after chemical induced wounding. […]
767

DNA photoprotection conveyed by sunscreen

Introduction:  Skin photoaging is the consequence of solar UV exposure, and DNA damage is an important part of this process. The objective of the current work was to demonstrate that in vitro skin models can be utilized to confirm that DNA damage protection is provided by sunscreens. Methods:   Skin equivalents were exposed to full-spectrum […]
535

POMEGRANATE DERIVED PRODUCTS FOR CANCER CHEMOPREVENTION.

This study by researchers in the University of Wisconsin’s Dept. of Dermatology used Mattek’s EpiDermFT full-thickness in vitro 3-D human skin tissue equivalent because it “sustains differentiation and exhibits morphological and metabolic properties similar to human skin”, to demonstrate the effect of a test substance applied to EpiDerm-FT to prevent skin cancer. Because treatment options […]
526

NFAT REGULATES INDUCTION OF COX-2 AND APOPTOSIS OF KERATINOCYTES IN RESPONSE TO ULTRAVIOLET RADIATION EXPOSURE.

This study by researchers at Newcastle Univesity and the Royal Victoria Infirmary demonstrated that Mattek’s EpiDermFT full-thickness in vitro human skin tissue equivalent is an excellent in vitro model to investigate the wavelengths of UV radiation that activate specific skin NFAT transcription factors and the biological function of UV activated NFAT. The nuclear factor of […]
727

NOVEL SILK FIBROIN/ELASTIN WOUND DRESSINGS.

Silk fibroin (SF) and elastin (EL) scaffolds were successfully produced for the first time for the treatment of burn wounds. The self-assembly properties of SF, together with the excellent chemical and mechanical stability and biocompatibility, were combined with elastin protein to produce scaffolds with the ability to mimic the extracellular matrix (ECM). Porous scaffolds were […]
532

MICROVESICATING EFFECTS OF SULFUR MUSTARD ON AN IN VITRO HUMAN SKIN MODEL.

This study by scientists at the US Army Medical Research Institute of Chemical Defense, University of Iowa, Harvard Medical School and Mattek Corp. demonstrated that Mattek’s EpiDermFT full-thickness in vitro 3-D human skin tissue equivalent with a well-developed basement membrane structure is a useful tool for in vitro mechanistic studies of sulfur mustard vesicant (blistering) […]
262

FULL THICKNESS EPIDERM™, A DERMAL-EPIDERMAL SKIN MODEL TO STUDY EPITHELIAL-MESENCHYMAL INTERACTIONS.

It has long been recognized that significant paracrine signaling occurs between dermal fibroblasts and epidermal keratinocytes. Further, it is understood that such interactions likely modulate the response of skin to materials which may lead to contact irritant or allergic responses. Also, dermis related phenomena such as photo-aging and photo-damage, wound healing and cancer progression are […]
781

Wound healing revised: A novel reepithelialization mechanism revealed by in vitro and in silico models

Wound healing is a complex process in which a tissue’s individual cells have to be orchestrated in an efficient and robust way. We integrated multiplex protein analysis, immunohistochemical analysis, and whole-slide imaging into a novel medium-throughput platform for quantitatively capturing proliferation, differentiation, and migration in large numbers of organotypic skin cultures comprising epidermis and dermis. […]
608

SULFUR MUSTARD TOXICITY FOLLOWING DERMAL EXPOSURE: ROLE OF OXIDATIVE STRESS, AND ANTIOXIDANT THERAPY.

Objective:  Sulfur mustard (bis-2-(chloroethyl) sulfide) is a chemical warfare agent (military code: HD) causing extensive skin injury. The mechanisms underlying HD-induced skin damage are not fully elucidated. This review will critically evaluate the evidence showing that oxidative stress is an important factor in HD skin toxicity. Oxidative stress results when the production of reactive oxygen […]
654

ORGANOTYPIC IN VITRO HUMAN EPITHELIAL MODELS WITH ENGINEERED TOXICOLOGIC REPORTER FUNCTIONS.

3D organotypic in vitro human epithelial models including skin (e.g. EpiDerm, EpiDerm-FT) and airway (e.g. EpiAirway, EpiAirway-FT) are important advances over traditional monolayer cell culture models. For toxicology applications, these models provide more realistic, in vivo-like structure, barrier properties, metabolic functions and dosing capabilities. In the current poster we describe development of organotypic human epithelial […]
300

IN VITRO MODELS OF FULL-THICKNESS HUMAN SKIN (EPIDERMFT™) AND AIRWAY EPITHELIUM (EPIAIRWAYFT™) FOR TOXICOLOGY AND DRUG DEVELOPMENT APPLICATIONS.

In vitro models of human skin and airway epithelia have potential applications in toxicology studies involving chemical warfare agents, environmental chemicals or consumer products, as well as in development of therapeutics delivered via dermal or inhalation routes. However the response of epithelial cells to exogenous chemical exposure is believed to be strongly influenced by paracrine […]
669

EVALUATION OF ANTI-INFLAMMATORY AND ATROPHOGENIC EFFECTS OF GLUCOCORTICOIDS ON RECONSTRUCTED HUMAN SKIN.

Topical glucocorticoids (GCs) are extensively used in the treatment of inflammatory skin diseases. However, their long-term use is often accompanied by severe and eventually irreversible adverse effects, with atrophy being the most important limitation. Currently, most non-clinical studies involve animal testing, so the results are not always representative of the situation in humans. The aim […]
473

HUMAN SKIN RESPONSES TO UV RADIATION: PIGMENT IN THE UPPER EPIDERMIS PROTECTS AGAINST DNA DAMAGE IN THE LOWER EPIDERMIS AND FACILITATES APOPTOSIS.

This study by researchers at the National Cancer Institute and the US Food and Drug Administration demonstrated how Mattek’s MelanoDerm reconstructed 3-D in vitro human skin tissues containing both keratinocytes and melanocytes could be used to investigate how increased melanin concentrations in human skin can act as a UV radiation “filter”, a phenomenon first seen […]
471

SOX9 IS A KEY PLAYER IN ULTRAVIOLET B-INDUCED MELANOCYTE DIFFERENTIATION AND PIGMENTATION.

This study by researchers from the National Cancer Institute (USA) and the Universite de Nice Sophia–Antipolis (France) demonstrated the ability to use MelanoDerm, one of Mattek’s re-constructed skin models, as a surrogate to assess the effect of UVB radiation on SOX9 expression in human skin in vivo. SOX (SRY type HMG box) proteins are transcription […]
683

UPDATE ON VALIDATION STATUS AND INDUSTRY UTILIZATION OF NORMAL HUMAN 3D (NHU-3D) ANIMAL ALTERNATIVE MODELS.

Progress in in vitro tissue engineering over the last several decades has contributed to a significant decrease in animal use for industrial risk and safety assessment of products and ingredients. Mattek Corporation has a long-standing involvement in development, production and validation of animal alternative in vitro models produced from normal human cells (NHu-3D models). The […]
691

CONFOCAL MICROSCOPY FOR MODELING ELECTRON MICROBEAM IRRADIATION OF SKIN.

For radiation exposures employing targeted sources such as particle microbeams, the deposition of energy and dose will depend on the spatial heterogeneity of the sample. Although cell structural variations are rela- tively minor for two-dimensional cell cultures, they can vary significantly for fully differentiated tissues. Employ- ing high-resolution confocal microscopy, we have deter- mined the […]
486

EVALUATION OF EPIDERM FULL THICKNESS-300 (EFT-300) AS AN IN VITRO MODEL FOR SKIN IRRITATION.

This study by scientists at the Colleges of Pharmacy at Florida A&M University and Auburn University, and at Mattek Corp. demonstrated that Mattek’s EpiDerm-FT full thickness human skin tissue equivalent is an excellent alternative to the use of animals for skin irritation research, in part because it contains a dermal as well as an epidermal […]
442

REGULATORY REQUIREMENTS FOR IN VITRO SYSTEMS TO MEET PERFORMANCE STANDARDS OVER TIME AS WELL AS DURING VALIDATION.

This technical poster by scientists at Mattek Corp. summarized 12-PLUS YEARS (1996-2007) of commercial production QC data for Mattek’s EpiDerm and EpiOcular in vitro human tissue equivalents, and how that data addresses regulatory concerns regarding performance standards of alternative in vitro testing methods over time. In vitro models are being proposed as alternatives to animal […]
400

NEAR-INFRARED OPTICAL SENSORS BASED ON SINGLE-WALLED CARBON NANOTUBES.

This study by researchers in the Chemical/Biomolecular Engineering and Chemistry departments at the University of Illinois demonstrated that Mattek’s EpiDermFT full-thickness in vitro human skin tissue equivalent can be used to mimic in vivo human skin tissues in NIR imaging studies. Molecular detection using near-infrared light between 0.9 and 1.3 eV has important biomedical applications […]
364

DEVELOPMENT OF A FULL THICKNESS IN VITRO HUMAN SKIN EQUIVALENT (EPIDERM-FT) FOR SULFUR MUSTARD RESEARCH.

This study by scientists at the U.S. Army Medical Research Institute of Chemical Defense and Mattek Corp. demonstrated that Mattek’s EpiDermFT full-thickness in vitro human skin tissue equivalent is an excellent in vitro alternative for studying the effects of human skin exposure to mustard gas. Skin exposure to the chemical warfare agent bis-(â-chloroethyl)sulfide (mustard gas) […]
609

THE SKIN CANCER CHEMOTHERAPEUTIC AGENT INGENOL-3-ANGELATE (PEP005) IS A SUBSTRATE FOR THE EPIDERMAL MULTIDRUG TRANSPORTER (ABCB1) AND TARGETS TUMOR VASCULATURE.

Ingenol-3-angelate (Ing3A), extracted from Euphorbia peplus, is currently in clinical trials for eradicating basal cell carcinoma, actinic keratosis, and squamous cell carcinoma (SCC) in situ by topical application. Although structurally related to phorbol esters and a protein kinase C activator, topical Ing3A, but not phorbol 12-myristate 13-acetate (PMA), inhibited the growth of subcutaneous tumors derived […]
422

PHOTOPROTECTIVE EFFECT OF ISOFLAVONE GENISTEIN ON ULTRAVIOLET B-INDUCED PYRIMIDINE DIMER FORMATION AND PCNA EXPRESSION IN HUMAN RECONSTITUTED SKIN AND ITS IMPLICATIONS IN DERMATOLOGY AND PREVENTION OF CUTANEOUS CARCINOGENESIS.

This study by researchers at Mount Sinai School of Medicine demonstrated that Mattek’s EpiDermFT epidermal/dermal human skin tissue equivalent can be used to investigate the photoprotective efficacy of compounds by exposing treated and untreated EpiDermFT tissues to UVB radiation. Genistein, the most abundant isoflavone of the soy derived phytoestrogen compounds, is a potent antioxidant and […]
403

EFFECTS IN UNIRRADIATED HUMAN TISSUE INDUCED BY RADIATION DAMAGE UP TO 1 MM AWAY.

This study by researchers at Columbia University’s Center for Radiological Research, Finland’s Radiation and Nuclear Safety Authority, and New York City’s Stuyvesant High School demonstrated how Mattek’s EpiDerm in vitro human skin tissue equivalent and EpiDermFT full-thickness in vitro human skin tissue equivalent can be used in vitro to study “bystander effects” of ionizing radiation […]
327

DETECTING REACTIVE OXYGEN SPECIES IN SKIN USING TWO-PHOTON FLUORESCENCE IMAGING MICROSCOPY.

Reactive oxygen species (ROS) contribute to skin photodamage including photoaging, immunomodulation, actinic keratosis and skin cancers. Because these highly-reactive derivatives of molecular oxygen are extremely short-lived and essentially non-emissive, they are difficult to detect directly. In addition, until recently with the realization of two-photon excited fluorescence (TPEF) imaging, the opaque and heterogeneous environment of the […]
389

INFLUENCE OF SOYBEAN SYMBIOSOME EXTRACT ON UV-INDUCED SKIN FREE-RADICAL BIOCHEMISTRY.

This study by researchers at Arch Personal Care Products, Arch Chemicals, Bioinnovation Laboratories, Inc., and Dermscan Group demonstrated how Mattek’s EpiDermFT full-thickness in vitro human skin tissue equivalent can be used to measure the ability of a topically-applied formulation to prevent skin damage by inhibiting the expression of nitric oxide free radicals in human (dermal […]
373

PHOTOPROTECTIVE EFFICACY OF ISOFLAVONE GENISTEIN IN HUMAN RECONSTITUTED SKIN.

This study by researchers in the Mount Sinai School of Medicine Department of Dermatology demonstrated that Mattek’s EpiDermFT full-thickness in vitro human skin tissue equivalent is an excellent in vitro alternative for studying the chemopreventive efficacy of topically applied anti-oxidants relative to UVB radiation exposure. Genistein, the soy plant derived isoflavone, has garnered significant noteworthy […]
339

HUMAN SKIN EQUIVALENT: A RELIABLE IN VITRO MODEL FOR CARCINOGENIC EXPERIMENTS.

EpiDermFT (Mattek Co., MA) is an in vitro model for human skin derived from keratinocytes of human neonatal foreskin. The epidermal component is cultured to form a highly differentiated model of metabolically and mitotically active cells that are arranged analogous to those found in human epidermal skin. This advanced full-thickness model incorporates a fibroblast-containing dermis […]
365

RECONSTRUCTED HUMAN SKIN MODEL TO STUDY MELANOMA AT DIFFERENT STAGES OF PROGRESSION.

This study by scientists at Mattek Corp. demonstrated that Mattek’s EpiDerm-FT in vitro human full-thickness skin tissue equivalent can be co-cultured with melanocytes at different stages of malignancy to produce three dimensional, serum-free tissue culture models that can be used to study, understand, and develop preventative and therapeutic treatments for cutaneous melanoma. The incidence of […]
326

HUMAN MELANOMA DEVELOPMENT IN RECONSTRUCTED SKIN MODELS.

Human skin reconstructs are three dimensional in vitro models consisting of a co-culture of epidermal keratinocytes and melanocytes plated onto a dermal substrate. In contrast to cells in monolayer culture, tissue-engineered skin equivalents contain well-developed epidermal layers with normal melanocytes being distributed at the basement membrane that separates the epidermis from the dermis. The incidence […]
718

COMPARISON OF LOW AND HIGH DOSE IONISING RADIATION USING TOPOLOGICAL ANALYSIS OF GENE COEXPRESSION NETWORKS.

Background:  The growing use of imaging procedures in medicine has raised concerns about exposure to low­ dose ionising radiation (LDIR). While the disastrous effects of high dose ionising radiation (HDIR) is well documented, the detrimental effects of LDIR is not well understood and has been a topic of much debate. Since little is known about […]
722

QUANTITATIVE PHOSPHOPROTEOMICS IDENTIFIES FILAGGRIN AND OTHER TARGETS OF IONIZING RADIATION IN A HUMAN SKIN MODEL.

Our objective here was to perform a quantitative phosphoproteomic study on a reconstituted human skin tissue to identify low- and high-dose ionizing radiation-dependent signalling in a complex three-dimensional setting. Application of an isobaric labelling strategy using sham and three radiation doses (3, 10, 200 cGy) resulted in the identification of 1052 unique phosphopeptides. Statistical analyses […]
660

SULFUR MUSTARD-INDUCED RESPONSES OF AIRWAY AND SKIN IN VITRO.

Sulfur mustard (SM) is a vesicant that has been used in warfare and is a potential threat to civilians either from acts of terrorism or through accidental exposure to abandoned military caches of the chemical. Exposure of the skin produces blisters and slow-healing wounds resulting in scarring, while exposure of the lung causes short-term sloughing […]
546

OXIDATIVE STRESS AND INFLAMMATORY RESPONSE IN DERMAL TOXICITY OF SINGLE-WALLED CARBON NANOTUBES.

This study by researchers at PPRB/NIOSH, West Virginia University and the University of Pittsburgh demonstrated that Mattek’s EpiDermFT full-thickness in vitro 3-D human skin tissue equivalent can be used to measure the dermal toxicity of single-walled carbon nanotubes (SWCNT). Single-walled carbon nanotubes (SWCNT) represent a novel material with unique electronic and mechanical properties. The extremely […]
684

USE OF NORMAL HUMAN 3D (NHU-3D) TISSUE MODELS (EPIDERM™, EPIAIRWAY™) FOR NANOTOXICOLOGY APPLICATIONS

Nanomaterials are increasingly utilized in numerous commercial applications where dermal contact, inhalation, or oral ingestion is likely. However, their toxicological properties are largely unknown. Potential adverse effects of nanoparticle exposure include allergenicity, cytotoxicity, and genotoxicity. Nanomaterials may enter the body by interacting with, and eventually crossing, epithelial barriers including skin, airway, and intestinal epithelium. Once […]
650

PARP-1 ACTIVITY IS INVOLVED IN THE SOLAR UV-INDUCED CUTANEOUS INFLAMMATORY RESPONSE IN THE EPIDERM-FT IN VITRO HUMAN SKIN MODEL.

Poly (ADP-ribose) polymerase 1 (PARP-1) is known to be activated under conditions of genotoxic stress.  In the presence of DNA damage, PARP-1, a nuclear zinc-finger DNA-binding protein, is activated and catalyzes the transfer of ADP-ribose from NAD+ to itself (automodification) and a range of other nuclear proteins (1,2,3).  UV-induced cutaneous inflammation has been demonstrated in […]
635

Th17 CYTOKINES INTERLEUKIN (IL)-17 AND IL-22 MODULATE DISTINCT INFLAMMATORY AND KERATINOCYTE-RESPONSE PATHWAYS.

Background Psoriasis vulgaris is an inflammatory skin disease mediated by Th1 and Th17 cytokines, yet the relative contribution. of interferon (IFN)-γ, interleukin (IL)-17 and IL-22 on disease pathogenesis is still unknown. Objectives: In this study, we sought to identify the cytokines produced by skin­-resident T cells in normal skin, localize the receptors for these cytokines, […]
613

EXPRESSION OF PROLIFERATIVE AND INFLAMMATORY MARKERS IN A FULL-THICKNESS HUMAN SKIN EQUIVALENT FOLLOWING EXPOSURE TO THE MODEL SULFUR MUSTARD VESICANT, 2-CHLOROETHYL ETHYL SULFIDE.

Sulfur mustard is a potent vesicant that induces inflammation, edema and blistering following dermal exposure. To assess molecular mechanisms mediating these responses, we analyzed the effects of the model sulfur mustard vesicant, 2-chloroethyl ethyl sulfide, on EpiDerm-FT™, a commercially available full-thickness human skin equivalent. CEES (100–1000 ìM) caused a concentration-dependent increase in pyknotic nuclei and […]
537

ANALYSIS OF IONIZING RADIATION-INDUCED DNA DAMAGE AND REPAIR IN THREE-DIMENSIONAL HUMAN SKIN MODEL SYSTEM.

This study by researchers in the Columbia University College of Physicians and Surgeons Center for Radiological Research demonstrated that Mattek’s EpiDerm in vitro 3-D human skin tissue equivalent and EpiDermFT full-thickness in vitro 3-D human skin tissue equivalent can be used to study the damaging effects to DNA of ionizing radiation exposure. Knowledge of cellular […]
396

LONG-TERM BIOLOGICAL BYSTANDER EFFECTS OF MICROBEAM IRRADIATION IN 3D HUMAN TISSUE MODELS.

This study by researchers at the National Cancer Institute (NIH), University of Lethbridge (Canada) and Columbia University demonstrated that Mattek’s EpiDermFT and EpiAirway in vitro human tissue equivalents exhibited the “radiation-induced bystander response” thereby overcoming the deficiencies of cultured-cell studies. Irradiated cells can induce genomic instability in unirradiated neighboring cells in vitro. This phenomenon, called […]
705

γ-H2AX FORMATION IN THREE-DIMENSIONAL HUMAN SKIN AND CELL MODELS FOLLOWING INVITRO EXPOSURE TO SULFUR MUSTARD.

Sulfur mustard (2-2’-dichlorodiethyl sulfide, SM) is a cytotoxic chemical warfare agent known for its vesicating properties. SM exposure to human tissue results in DNA damage, eventually leading to cell and tissue death. To elucidate the genotoxic effects of SM, the presence of γ-H2AX foci was determined in several skin models. These included normal human epidermal […]
682

CHARACTERIZATION OF ENZYME ACTIVITIES OF CYTOCHROME P450 ENZYMES, FLAVIN-DEPENDENT MONOOXYGENASES, N-ACETYLTRANSFERASES AND UDP-GLUCURONYLTRANSFERASES IN HUMAN RECONSTRUCTED EPIDERMIS AND FULL-THICKNESS SKIN MODELS.

With the perspective to use human reconstructed skin models for genotoxicity testing which require metabolic activation of xenobiotics, this study aimed to characterize activities of biotransforming enzymes within two human reconstructed skin models, the epidermis model EpiDerm™ (Mattek) and the Phenion_Full-Thickness skin model Phenion_FT (Henkel). According to existing gene expression profiles, Cytochrome P450 (CYP) enzymes, […]
678

INVESTIGATION OF THE BIOTRANSFORMING CAPACITY OF HUMAN RECONSTRUCTED SKIN MODELS FOR GENOTOXICITY TESTING.

Skin penetration is a route of xenobiotic uptake in the body, however, less information is available about the fate of the penetrated xenobiotic in regard of metabolic turn over. The presence of xenobiotic metabolizing enzymes in skin has previously been shown by transcriptional expression analysis including CYPs, Flavin-dependent monooxygenases (FMO), Alcohol- (ADH) and Aldehyde-Dehydrogenases (ALDH), […]
730

METABOLICALLY COMPETENT HUMAN SKIN MODELS: ACTIVATION AND GENOTOXICITY OF BENZO[A]PYRENE.

The polycyclic aromatic hydrocarbon (PAH) benzo[a]pyrene (BP) is metabolized into a complex pattern of BP derivatives, among which the ultimate carcinogen (+)-anti-BP-7,8-diol-9,10- epoxide (BPDE) is formed to certain extents. Skin is frequently in contact with PAHs and data on the metabolic capacity of skin tissue toward these compounds are inconclusive. We compared BP metabolism in […]
658

EVALUATION OF XENOBIOTIC METABOLISM IN HUMAN RECONSTRUCTED SKIN MODELS FOR GENOTOXICITY TESTING.

Skin penetration and absorption is a major route of xenobiotic uptake. Skin maintains furthermore capacities to transform chemical substances which was previously shown by transcriptional expression analysis of metabolizing enzymes including CYPs, Flavin-dependent monooxygenases (FMO), N- Acetyltransferases (NAT) and UDP-Glucuronyltransferases (UDP-GT) [1-3]. Their catalytic activity in skin tissue however was rarely specified. Human reconstructed skin […]
621

GENOTOXICITY TESTING USING THE NORMAL HUMAN CELL BASED 3D EPITHELIAL MODELS.

Safety assessment of new products for human use requires genotoxicity testing to ensure their non-carcinogenicity. Current in vitro genotoxicity assays have low specificity resulting in unacceptably high rates of false positives. Moreover, starting in 2009, in vivo genotoxicity testing was banned by the 7th Amendment to the Cosmetics Directive. Since epithelial tissues have the highest […]
371

IN VITRO SKIN EQUIVALENT MODELS FOR TOXICITY TESTING.

This technical paper by Mattek scientists documented how Mattek’s EpiDerm, EpiDermFT and MelanoDerm in vitro human skin tissue equivalents can be used to assess the safety of topically applied pharmaceutical and cosmetic products. Prior to human use of new cosmetic or therapeutic products, in vivo animal tests are often conducted in order to screen for […]
623

TAT-MEDIATED DELIVERY OF A DNA REPAIR ENZYME TO SKIN CELLS RAPIDLY INITIATES REPAIR OF UV-INDUCED DNA DAMAGE.

UV light causes DNA damage in skin cells, leading to more than one million cases of non-melanoma skin cancer diagnosed annually in the United States. Although human cells possess a mechanism (nucleotide excision repair) to repair UV-induced DNA damage, mutagenesis still occurs when DNA is replicated before repair of these photoproducts. Although human cells have […]
560

EVALUATION OF EPIDERM FULL THICKNESS-300 (EFT-300) AS AN IN VITRO MODEL FOR SKIN IRRITATION: STUDIES ON ALIPHATIC HYDROCARBONS.

This study by scientists at Florida A&M University, Auburn University and Mattek Corp. demonstrated that Mattek’s EpiDermFT full-thickness in vitro 3-D human skin tissue equivalent is an excellent in vitro model for predicting in vivo irritation and understanding the structural activity relationship of saturated aliphatic hydrocarbons.The aim of this study was to understand the skin […]
604

PROTECTIVE EFFECT OF POMEGRANATE-DERIVED PRODUCTS ON UVB-MEDIATED DAMAGE IN HUMAN RECONSTITUTED SKIN.

Solar ultraviolet (UV) radiation, particularly its UVB (290–320 nm) component, is the primary cause of many adverse biological effects including photoageing and skin cancer. UVB radiation causes DNA damage, protein oxidation and induces matrix metalloproteinases (MMPs). Photochemoprevention via the use of botanical antioxidants in affording protection to human skin against UVB damage is receiving increasing […]
538

EXPRESSION OF STRATUM CORNEUM LIPID PATHWAYS IN HUMAN SKIN EQUIVALENT CULTURES.

This study by scientists at Procter & Gamble Beauty demonstrated that gene pathways involved in stratum corneum lipid formation are expressed in Mattek’s EpiDermFT full-thickness in vitro 3-D human skin tissue equivalent, suggesting that ths culture system is an appropriate in vitro approach for studying stratum corneum biology. Human skin equivalent cultures contain living human […]
726

CELL TYPE-DEPENDENT GENE TRANSCRIPTION PROFILE IN A THREE-DIMENSIONAL HUMAN SKIN TISSUE MODEL EXPOSED TO LOW DOSES OF IONIZING RADIATION: IMPLICATIONS FOR MEDICAL EXPOSURES.

The concern over possible health risks from exposures to low doses of ionizing radiation has been driven largely by the increase in medical exposures, the routine implementation of X-ray backscatter devices for airport security screening, and, most recently, the nuclear incident in Japan. Because of a paucity of direct epidemiological data at very low doses, […]
764

INGENOL MEBUTATE INDUCED CELL DEATH PATTERNS IN NORMAL AND CANCER EPITHELIAL CELLS

We investigated the proposed necrotic mechanism of ingenol mebutate, a natural compound with anti-cancer properties in human keratinocytes, the human squamous cell carcinoma cell line HSC-5, and HeLa cervix carcinoma cells. Topical application of a clinical dose of ingenol mebutate 0.05% (1.15 mM) gel to human reconstituted full-thickness skin equivalents strongly reduced epidermal, but not […]
640

SIMULATION OF ELECTRON-BEAM IRRADIATION OF SKIN TISSUE MODEL.

Monte Carlo simulation of electrons stopping in liquid water was used to model the penetration and quality of electron-beam irradiation incident on the full-thickness EpiDermTM skin model (EpiDermFTTM Mattek, Ashland, VA). This 3D tissue model has a fully developed basement membrane separating an epidermal layer of keratinocytes in various stages of differentiation from a dermal […]
599

A NEW DECORIN-LIKE TETRAPEPTIDE FOR OPTIMAL ORGANIZATION OF COLLAGEN FIBRES.

Decorin interacts with collagen via its protein core and influences collagen fibrillogenesis, thus regulating excessive bundle-like aggregation of collagen. As skin ages, there is lack of functional decorin, which results in disrupted collagen fibres and in a reduction in the tensile strength of the skin. Therefore, a substitute for decorin would make up for the […]
563

RELATIVE BIOLOGICAL EFFECTIVENESS OF PULSED AND CONTINUOUS 20 MEV PROTONS FOR MICRONUCLEUS INDUCTION IN 3D HUMAN RECONSTRUCTED SKIN TISSUE.

Background and purpose: Laser accelerated radiotherapy is a prospect for cancer treatment with proton and/or carbon ion beams that is currently under fast development. In principal, ultra fast, high-energy laser pulses will lead to a ‘‘pulsed” delivery of the induced ion beam with pulse durations of 1 ns and below, whereas conventional proton beams deriving […]
530

SKIN SCIENCE: THE USE OF GENOMICS TOOLS IN SKIN CARE.

This study by scientists at Genemarkers LLC demonstrated that Mattek’s EpiDermFT full-thickness in vitro human skin tissue equivalent can be used to identify the human genes activated when anti-aging and anti-wrinkle cosmeceutical products are applied to the apical surface. Elina Ambra-Lift Activates Genes that Promote Anti-Aging and Anti-Wrinkle Effects. The use of genomics to validate […]
467

SYNERGISTIC ACTIONS OF PEMPHIGUS VULGARIS IGG, FAS-LIGAND AND TUMOR NECROSIS FACTOR-A DURING INDUCTION OF BASAL CELL SHRINKAGE AND ACANTHOLYSIS.

This study by researchers at the University of California at Davis demonstrated that when Mattek’s EpiDerm-FT epidermal/dermal human skin tissue equivalents were treated with pemphigus vulgaris (PV) IgGs, it resulted in suprabasilar epidermal clefting and the cell detachment pattern characteristic of the acantholysis seen in the skin of pemphigus vulgaris patients. This study by researchers […]
618

PROTEOMIC ANALYSIS OF LASER MICRODISSECTED MELANOMA CELLS FROM SKIN ORGAN CULTURES.

Gaining insights into the molecular events that govern the progression from melanoma in situ to advanced melanoma and understanding how the local microenvironment at the melanoma site influences this progression are two clinically pivotal aspects that to date are largely unexplored. In an effort to identify key regulators of the crosstalk between melanoma cells and […]
723

BIOCHEMICAL INVESTIGATION AND GENE ANALYSIS OF EQUOL: A PLANT AND SOY-DERIVED ISOFLAVONOID WITH ANTIAGING AND ANTIOXIDANT PROPERTIES WITH POTENTIAL HUMAN SKIN APPLICATIONS.

The purpose of this study was to investigate the effects of equol, a plant and intestinal flora derived isoflavonoid molecule on the expression of skin genes and proteins using human dermal models. As equol has been shown to mimic 17β-estradiol and bind specifically to 5α-dihydrotestosterone (5α-DHT), these agents were used (in addition to equol) to […]
518

IN VITRO SKIN BIOMARKER RESPONSES TO A NEW ANTI-AGING PEPTIDE, PAL-KT.

This study by scientists at Procter & Gamble (P&G Beauty Div.) and Sederma demonstrated that Mattek’s EpiDermFT full-thickness in vitro human skin tissue equivalent is an excellent in vitro model for screening novel peptides for their potential anti-aging effects on facial skin. Peptides applied topically in moisturizer-type cosmetic products are known to improve the appearance […]
488

STIMULATION OF EPIDERMAL HYALURONAN BY YEAST FERMENT.

This study by researchers at Procter & Gamble Co. demonstrated how Mattek’s EpiDermFT full-thickness in vitro human skin tissue equivalent can be used to study the expression of a major extracellular matrix component of human skin (hyaluronan), and can also be used to trace hyaluronan expression to cells in the epidermal layer. INTRODUCTION: Hyaluronan is […]
460

EVALUATION OF COSMETIC INGREDIENTS IN TISSUE ENGINEERED HUMAN SKIN IN VITRO.

This study by scientists at Procter & Gamble demonstrated that Mattek’s MelanoDerm and EpiDermFT in vitro human skin equivalent tissues are useful tools in understanding how compounds used in anti-aging cosmetic products (“cosmeceuticals”) like N-acetyl glucosamine and niacinamide affect production of important skin matrix components including collagen. Introduction: As part of Procter & Gamble’s ongoing […]
549

AccelaFuze™ PEPTIDE INFUSION THERAPY.

This study by scientists at Woodford Medical Aesthetics demonstrated that Mattek’s EpiDermFT full-thickness in vitro 3-D human skin tissue equivalent, because it is produced from normal (non-transformed) human skin cells (keratinocytes and fibroblasts), can be used to accurately measure the specific gene expression changes produced by new topical skin treatments before they are tested on […]
634

UNDERSTANDING METABOLIC PATHWAYS FOR SKIN ANTI-AGING.

Global gene expression profiling provides a useful means to identify key aspects of the skin aging process, and provides Information to help develop new skin technologies. Important aspects of skin aging that can be addressed Include skin hydration, barrier, matrix, pigmentation and antioxidant capacity. Human skin equivalent cultures allow topical application of test compounds, combinations […]
510

TOPICAL N-ACETYL GLUCOSAMINE AND NIACINAMIDE INCREASE HYALURONAN IN VITRO.

This study by researchers at Procter & Gamble Co. demonstrated how Mattek’s EpiDermFT full-thickness in vitro human skin tissue equivalent can be used to study the expression of a major extracellular matrix component of human skin (hyaluronan) when N-acetyl glucosamine and niacinamide are applied to the apical surface of the tissues simulating topical application on […]
595

DC DETOXDEFENSE™ STIMULATES SKIN’S OWN SOD ANTIOXIDANT PROTECTION AND DETOXIFICATION.

Objective: The objective of this experiment was to determine whether the results previously obtained DC Detoxdefense in enzymatic and cell-based assays are corroborated by modulation of gene expression in EpiDerm human skin substitute model. Methods: EpiDerm tissues were obtained from Mattek (Ashland, MA) and cultured according to the manufacturer’s instructions. The test materials – Upregulex […]
385

USE OF EPIDERM FULL THICKNESS TISSUE FOR ANTI-AGING STUDIES.

This study by scientists at Arch Personal Care Products demonstrated that Mattek’s EpiDermFT full-thickness in vitro human skin tissue equivalent can be used to measure the effect of a new topically applied formulation on the elastin content in human skin’s extracellular matrix. Mattek’s EpiDerm and EpiOcular in vitro tissues were also used to show this […]
738

PROTECTION AND REPAIR OF SKIN DAMAGE FROM ENVIRONMENTAL AGGRESSORS.

There is an increasing awareness that environmental factors, such as UV, cigarette smoke, pollution, and extreme temperatures can lead to skin aging. The mechanisms underlying this extrinsic aging effect remain uncertain. Previous studies showed that one of the negative actions from UV and smoke was oxidative damage. Here, we show that each of the aggressors […]
737

BIOLOGICAL FUNCTION & MODULATION OF THYMOSIN β4 IN HUMAN SKIN.

Thymosin beta 4 (Tb4) belongs to a highly conserved, water-soluble, acidic polypeptide family of proteins1. Although it was originally identified as an actin-sequestering molecule in eukaryotic cells, Tb4 plays a critical role in dermal and corneal wound healing via multiple activities: anti-inflammatory, stimulation of keratinocyte migration, regulating MMP expression, and matrix remodeling2, 3, 4. Age-related […]
736

ENCAPSULATED RETINOL (0.5%) AND RETINOIC ACID (0.05%) ARE EQUALLY EFFECTIVE AT PROTECTING AGAINST UV-INDUCED DAMAGE IN EPIDERM-FT TISSUES.

Solar ultraviolet light damages skin resulting in photoaging which superimposed on chronological aging resulting in worsening of skin appearance. The extent of sun damage is proportional to the amount/time of UV-light exposure triggering visible changes such as dryness, rough texture, irregular pigmentation, telangiectasia, plaque-like thickening, deep creases and fine wrinkles. Both epidermis and dermis are […]
735

DIFFERENTIAL MODULATION OF ECM COMPONENTS AND GFR BY RETINOIC ACID AND A PHYSIOLOGICALLY BALANCED GF FORMULATION.

Overall appearance of skin is a combination of health, ethnicity, lifestyle and age, which determine skin color, texture, firmness and smoothness. Uneven color, rough, lax and wrinkled skin are associated with aged skin. Microscopically, aged skin has an atrophic epidermis, flattened dermal-epidermal junction, decreased number of dermal fibroblasts and diminished synthesis of extracellular matrix components […]
495

RECONSTRUCTED EPIDERMIS AND FULL-THICKNESS SKIN FOR ABSORPTION TESTING: INFLUENCE OF THE VEHICLES USED ON STEROID PERMEATION.

This study by researchers at the Free University of Berlin demonstrated that Mattek’s EpiDerm in vitro human skin tissue equivalent and EpiDermFT full-thickness in vitro human skin tissue equivalent can be used in the development of topical dermatics and for hazard analysis of toxic compounds and their formulations. A protocol for percutaneous absorption studies has […]
784

Evaluation of Cutaneous Damage and Repair Following Acute Solar Ultraviolet Radiation Exposure: Experiments with an In Vitro Reconstructed Human Skin Model and Excised Human Skin

Overexposure of human skin to solar ultraviolet radiation (UVR) is known to produce deleterious effects including cutaneous photoaging and cancers. The present studies were conducted to confirm the utility of a commercially available full-thickness reconstructed human skin model (EpiDermFT) for reproducing tissue and cellular effects involved in cutaneous responses to acute solar UVR exposure. EpiDermFT […]