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EpiAirwayFT

1007

Use of In Vitro Human Skin Models to Assess Potential Immune Activation In Response to Biotherapeutic Attributes and Process-related Impurities

Subcutaneous (SubQ) injection is a common administration route for biotherapeutics. However, limited tools are available for understanding the dynamic relationships between drug products and resident cells following injection. Advances in tissue engineering have enabled the production of in vitro skin models that recapitulate the morphological structure and functional activity of human skin. Here we explore […]
960

Comparison of 3D airway models for the assessment of fibrogenic chemicals

Lung epithelial cells and fibroblasts play key roles in pulmonary fibrosis and are involved in fibrotic signaling and production of the extracellular matrix (ECM), respectively. Recently, 3D airway models consisting of both cell types have been developed to evaluate the fibrotic responses while facilitating cell–cell crosstalk. This study aimed to evaluate the fibrotic responses in […]
954

Petroleum coke exposure leads to altered secretome profiles in human lung models

Petroleum coke (PC) is a coal-like product that is produced during the refinement of crude oil and bituminous sand. Fugitive dust from open storage of PC in urban areas is a potential human health concern. Animal inhalation studies suggest that PC leads to an adverse pulmonary histopathology, including areas of fibrosis and chronic inflammation; however, […]
835

In Vitro Human Airway Models for Study of Goblet Cell Hyperplasia and Mucus Production: Effects of Th2 Cytokines, Double-Stranded RNA, and Tobacco Smoke

Goblet cell hyperplasia (GCH) within the epithelium of the conducting airways is a common feature of human respiratory diseases, including asthma and chronic obstructive pulmonary disease. GCH can be initiated or exacerbated by environmental agents, including air pollution, viral infection, and tobacco smoke. Experimental model systems for investigating causes of GCH and possible remedies are therefore of high […]
570

TIMECOURSE OF TH2 CYTOKINE-INDUCED GOBLET CELL HYPERPLASIA IN THE EPIAIRWAY™ IN VITRO HUMAN AIRWAY MODEL.

INTRODUCTION: Goblet cell hyperplasia and associated airway re-modeling contribute significantly to the pathogenesis of airway diseases such as asthma, rhinitis and COPD. TH2 cytokines including IL-4 and IL-13 are known to induce goblet cell hyperplasia in various in vivo animal models and in vitro airway cell model systems. However, the timecourse of induction and recovery […]
300

IN VITRO MODELS OF FULL-THICKNESS HUMAN SKIN (EPIDERMFT™) AND AIRWAY EPITHELIUM (EPIAIRWAYFT™) FOR TOXICOLOGY AND DRUG DEVELOPMENT APPLICATIONS.

In vitro models of human skin and airway epithelia have potential applications in toxicology studies involving chemical warfare agents, environmental chemicals or consumer products, as well as in development of therapeutics delivered via dermal or inhalation routes. However the response of epithelial cells to exogenous chemical exposure is believed to be strongly influenced by paracrine […]
683

UPDATE ON VALIDATION STATUS AND INDUSTRY UTILIZATION OF NORMAL HUMAN 3D (NHU-3D) ANIMAL ALTERNATIVE MODELS.

Progress in in vitro tissue engineering over the last several decades has contributed to a significant decrease in animal use for industrial risk and safety assessment of products and ingredients. Mattek Corporation has a long-standing involvement in development, production and validation of animal alternative in vitro models produced from normal human cells (NHu-3D models). The […]
660

SULFUR MUSTARD-INDUCED RESPONSES OF AIRWAY AND SKIN IN VITRO.

Sulfur mustard (SM) is a vesicant that has been used in warfare and is a potential threat to civilians either from acts of terrorism or through accidental exposure to abandoned military caches of the chemical. Exposure of the skin produces blisters and slow-healing wounds resulting in scarring, while exposure of the lung causes short-term sloughing […]
684

USE OF NORMAL HUMAN 3D (NHU-3D) TISSUE MODELS (EPIDERM™, EPIAIRWAY™) FOR NANOTOXICOLOGY APPLICATIONS

Nanomaterials are increasingly utilized in numerous commercial applications where dermal contact, inhalation, or oral ingestion is likely. However, their toxicological properties are largely unknown. Potential adverse effects of nanoparticle exposure include allergenicity, cytotoxicity, and genotoxicity. Nanomaterials may enter the body by interacting with, and eventually crossing, epithelial barriers including skin, airway, and intestinal epithelium. Once […]
663

MECHANISMS OF GOBLET CELL HYPERPLASIA INDUCED BY SIMULATED VIRAL EXPOSURE OR TH2 CYTOKINES.

Introduction:  Goblet cell hyperplasia is a common feature of respiratory epithelial response to numerous environmental challenges (e.g. cigarette smoke, air pollution, viral infection) and is also commonly observed in respiratory diseases, such as chronic obstructive pulmonary disease (COPD) and asthma (1-4).  In the current work we sought to reproduce goblet cell hyperplasia in an organotypic […]