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Pigmentation studies

1021

16-Kauren-2-beta-18,19-triol inhibits melanosome transport in melanocytes by down-regulation of melanophilin expression

Rab27a, Mlph, and MyoVa form a tripartite complex and relate to melanosome distribution. Melanophilin (Mlph) acts as a linker protein between Rab27a and MyoVa. The biological activity and function of 16-kauren on the expression of Mlph has not yet been studied.
877

Antimelanogenic Efficacy of Melasolv (3,4,5-Trimethoxycinnamate Thymol Ester) in Melanocytes and Three-Dimensional Human Skin Equivalent

Background/Aims: Excessive melanogenesis often causes unaesthetic hyperpigmentation. In a previous report, our group introduced a newly synthesized depigmentary agent, Melasolv TM (3,4,5-trimethoxycinnamate thymol ester). In this study, we demonstrated the significant whitening efficacy of Melasolv using various melanocytes and human skin equi valents as in vitro experimental systems. Methods: The depigmentary effect of Melasolv was […]
869

Coumestrol Down-Regulates Melanin Production in Melan-a Murine Melanocytes through Degradation of Tyrosinase

Pigmentation reflects skin darkening caused by melanin production, but excessive melanin synthesis may cause problems, such as melasma, solar lentigo, dark spots, and freckles. Considerable effort has been devoted to alleviating these undesired symptoms through the development of safe and effective depigmenting agents. Coumestrol, a plant-derived natural isoflavone with an estrogen-like structure and actions, is […]
851

Measurement of Skin Pigmentation Using a Chromameter in a 3-Dimensional Epidermal Model Containing Functional Melanocytes

Cosmetic or skin care pharmaceutical formulations augment skin pigmentation either for the intended purpose of skin lightening or as an off-target effect.   A convenient way to screen such effects utilizes the MelanoDerm tissue model, a highly differentiated, three-dimensional tissue culture model of human epidermis containing normal human melanocytes and keratinocytes.  Use of this model can […]
850

Standardization of In vitro Macrophotography for Assessment of Cutaneous Responses

The increased popularity of commercially available 3-dimensional human skin equivalents in recent years has allowed for assessment of melanogenesis modulated by compounds topically applied to the skin or directly incorporated from the medium. These skin equivalents provide a suitable model for elucidating the mechanisms of action of various factors that modulate skin pigmentation or other […]
828

Topical Application of Self-delivering RNAi (sd-rxRNA®) Compounds for Reduction of Hyperpigmentation

There are a number of dermal hyperpigmentation disorders that have a potentially negative impact on a person’s psychological well-being. These disorders are caused by an over-production of melanin. The enzyme tyrosinase catalyzes the rate-limiting steps in the production of melanin by melanocytes and inhibition of tyrosinase results in a reduction in pigmentation. We have developed […]
695

HYPERPIGMENTATION IN HUMAN SOLAR LENTIGO IS PROMOTED BY HEPARANASE-INDUCED LOSS OF HEPARAN SULFATE CHAINS AT THE DERMAL–EPIDERMAL JUNCTION.

Background:  Skin pigmentation induced by ultraviolet B radiation is caused in part by inflammation mediated by cytokines secreted from keratinocytes and fibroblasts in the irradiated area. Heparanase is also activated in the irradiated skin, and this leads to loss of heparan sulfate at the dermal–epidermal junction (DEJ), resulting in uncontrolled diffusion of heparan sulfate-binding cytokines […]
694

FGF2 REGULATES MELANOCYTES VIABILITY THROUGH THE STAT3-TRANSACTIVATED PAX3 TRANSCRIPTION.

PAX3 (paired box 3) is known to have an important role in melanocyte development through modulation of microphthalmia­associated transcription factor transcription. Here we found that PAX3 transcriptional activity could be regulated through FGF2 (basic fibroblast growth factor)-STAT3 (signal transducer and activator of transcription 3) signaling in the pigment cells. To study its function in vivo, […]
660

SULFUR MUSTARD-INDUCED RESPONSES OF AIRWAY AND SKIN IN VITRO.

Sulfur mustard (SM) is a vesicant that has been used in warfare and is a potential threat to civilians either from acts of terrorism or through accidental exposure to abandoned military caches of the chemical. Exposure of the skin produces blisters and slow-healing wounds resulting in scarring, while exposure of the lung causes short-term sloughing […]
698

EBSELEN IS A NEW SKIN DEPIGMENTING AGENT THAT INHIBITS MELANIN BIOSYNTHESIS AND MELANOSOMAL TRANSFER.

We assessed the ability of ebselen, a glutathione peroxidase mimic, to reduce pigmentation in various models. In murine B16 melanocytes, 25 µm ebselen inhibited melanogenesis and induced a depolymerisation of actin filaments. In co-cultures of B16 melanocytes with BDVII keratinocytes, a pretreatment of melanocytes with ebselen resulted in a strong inhibition of melanosome transfer to […]