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Oral irritation

1091

In vitro cytotoxicity assessment and mechanistic insight of EpiOral™ tissue model to oral nicotine products with extraction stability analysis

Cell models applicable to the oral route of use are important for analysing cytotoxicity of oral nicotine products. MatTek™ EpiOral tissues were utilised when testing these products in the MTT [3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide] assay. Products were extracted in complete artificial saliva (CAS) to mimic product use. Extractions were performed on 300mg of product per mL in […]
1066

Development of an In Vitro Method for Assessing the Potential Irritation of Medical Devices and OTC Products Used in the Oral Cavity

The aim of this study was to evaluate an in vitro method using the EpiOralTM model, a three-dimensional cultured human buccal epithelium, for assessing the oral irritation potential of various products. We evaluated different concentrations of nine chemicals commonly found in over-the-counter (OTC) products and medical devices, including chlorhexidine digluconate, sodium hypochlorite, phosphoric acid, hydrogen peroxide, […]
1063

Exploring the potential of reconstructed human epithelial tissue models for safety assessment of intraoral medical devices

Medical devices are integral to a wide array of medical interventions and are increasingly utilized in both clinical and home settings. Within the oral cavity, intraoral medical devices are employed for various applications, to improve quality of life and maintain oral health and hygiene. However, the dynamic and complex environment of the oral cavity, characterized […]
1055

Sensitivity Analysis and Quality Indicators for an In Vitro Oral Irritation Assay

Biocompatibility testing using in vivo tests is often one of the final evaluations of new dental materials. To reduce the likelihood of failure at this late stage, predictive biocompatibility testing using in vitro methods is needed. In this study, we describe a sensitivity analysis of an oral irritation test by evaluating changes in the viability, […]
795

Toxicity and Inflammatory Response to Common Magic Mouthwashes in a 3D Oral Reconstructed Tissue Model

Magic Mouthwash is a general term describing oral rinses prescribed by a doctor for the treatment of pain, inflammation, or infection, commonly as a result of chemotherapy and radiation induced oral sores (oral mucositis). There is no set combination of ingredients for the preparation of a magic mouthwash, rather they are formulated for the individual […]
1025

Safety assessment of monographed OTC cold/cough medicine using an in vitro testing platform based on human reconstructed oral tissues

Over-the-counter (OTC) products are available to alleviate concurrent symptoms of colds and flu. They are primarily based on a combination of decongestants, antitussive and alpha adrenergic agonists, which are well-established pharmaceutical agents covered by U.S. monographs. Many of the active components of the OTC cough/cold drugs are bitter and must be masked using flavoring agents. […]
995

Development of an oral mucosal irritation test using a three-dimensional human buccal oral mucosal model

 The oral mucosa can become irritated by oral care products and lip cosmetics. Therefore, it is important to determine the irritation potential of their ingredients and products during safety evaluations. We developed a method for oral mucosal irritation test using EpiOral, which is a three-dimensional cultured model. Exposure of sodium lauryl sulphate (SLS) to EpiOral […]
982

Cytokine Release from Organotypic Human Oral Tissue Following Exposure to Oral Care Product Chemicals

Purpose: Measuring viability of a three-dimensional in vitro organotypic human oral tissue model has been suggested as an alternative test method to the oral mucosa irritation test of oral care products. The aim of this study was to investigate the production of two different cytokines using organotypic human oral tissue model following exposure to chemicals […]
919

In vitro three-dimensional organotypic culture models of the oral mucosa

Three-dimensional, organotypic models of the oral mucosa have been developed to study a wide variety of phenomena occurring in the oral cavity. Although a number of models have been developed in academic research labs, only a few models have been commercialized. Models from academic groups offer a broader range of phenotypes while the commercial models […]
870

E-cigarettes and flavorings induce inflammatory and prosenescence responses in oral epithelial cells and periodontal fibroblasts

Electronic-cigarettes (e-cigs) represent a significant and increasing proportion of tobacco product consumption, which may pose an oral health concern. Oxidative/carbonyl stress via protein carbonylation is an important factor in causing inflammation and DNA damage. This results in stress-induced premature senescence (a state of irreversible growth arrest which re-enforces chronic inflammation) in gingival epithelium, which may […]
831

Oral Irritation Assessment of Electronic Liquids using an In-Vitro Oral Testing Model

While data are still being collected and analyzed, there were at least 1,300 accidental electronic liquid (e-liquid) exposures reported as of 2013. Deaths have occurred as a result of ingestion of e-liquid with the effects being attributed primarily to nicotine. The Food and Drug Administration (FDA) sought to regulate e-liquid through the Tobacco Control Act […]
811

Systems Toxicology Assessment of the Biological Impact of a Candidate Modified Risk Tobacco Product on Human Organotypic Oral Epithelial Cultures

Cigarette smoke (CS) has been reported to increase predisposition to oral cancer and is also recognized as a risk factor for many conditions including periodontal diseases, gingivitis, and other benign mucosal disorders. Smoking cessation remains the most effective approach for minimizing the risk of smoking-related diseases. However, reduction of harmful constituents by heating rather than combusting tobacco, without modifying the amount […]
842

In vitro systems toxicology approach to investigate the effects of repeated cigarette smoke exposure on human buccal and gingival organotypic epithelial tissue cultures

Smoking has been associated with diseases of the lung, pulmonary airways and oral cavity. Cytologic, genomic and transcriptomic changes in oral mucosa correlate with oral preneoplasia, cancer and inflammation (e.g. periodontitis). Alteration of smoking-related gene expression changes in oral epithelial cells is similar to that in bronchial and nasal epithelial cells. Using a systems toxicology approach, we have previously […]
3290

Transcriptional profiling of radiation damage and preventive treatments in a 3-dimensional (3D) human cell culture model of oral mucositis

Cancer patients who receive radiation are often afflicted by oral mucositis, a debilitating disease, characterized by mouth sores and difficulty in swallowing. Oftentimes, cancer patients afflicted with mucositis must stop lifesaving therapies. Thus it is very important to prevent mucositis before it develops. Using a validated organotypic model of human oral mucosa, a 3-dimensional cell culture model of […]
507

DEVELOPMENT OF AN EPIORAL IN VITRO HUMAN TISSUE MODEL FOR ORAL IRRITANCY TESTING.

This study by researchers at British American Tobacco and Advanced Technologies Ltd. demonstrated that Mattek’s EpiOral in vitro human buccal (inner cheek) tissue equivalent is an excellent in vitro model for analyzing the human oral irritation potential of tobacco products. The EpiOral™ tissue model (ORL-200), developed by the Mattek Corporation (Ashland, MA, USA), consists of […]
263

GINGIVAL TISSUE MODEL FOR PREDICTION OF IRRITANCY OF ORAL CARE PRODUCTS.

The development of human reconstructed tissues of the oral cavity that express in vivo-like differentiated characteristics would be important to better understand and develop therapies for gum disease, oral cancer, lesions due to smokeless tobacco, and gingivitis, amongst other diseases of the oral mucosa. In vitro tissue models would be used to assess the biocompatibility […]
379

EPIGINGIVAL™ (GIN-100) TISSUE MODELS FOR ORAL IRRITATION STUDIES.

This study by scientists at the University of Washington, University of Iowa and Mattek Corp. demonstrated that Mattek’s EpiOral and EpiGingival in vitro human oral tissue equivalents can be used to provide a quick, reproducible method for evaluating the irritation potential of oral care excipients and oral care products, that the methodology correlates well to […]
360

NOVEL IN VITRO METHODOLOGY EVALUATING POTENTIAL MUCOSAL IRRITATION OF ORAL CARE FORMULATIONS.

This study by researchers at The Procter & Gamble Co. demonstrated that Mattek’s EpiOral in vitro human buccal (inner cheek) tissue equivalent provided a quick, reproducible method for evaluating the irritation potential of oral care excipients and prototype formulations, the methodology correlated well to human irritation results, and that this EpiOral-based methodology proved to be […]
399

CHARACTERIZATION AND TESTING OF NEW BUCCAL AND GINGIVAL TISSUE MODELS.

This study by scientists at the University of Washington, University of Iowa and Mattek Corp. demonstrated that Mattek’s EpiOral and EpiGingival in vitro human buccal and gingival oral tissue equivalents showed good correspondence to human explant buccal and gingival tissue structure, protein expression, and lipid content; reproducibility of the tissues was very good; and differences […]
778

Evaluation of a Reconstructed Human Oral Buccal Tissue Model as a Testing Platform for Determining the Oral Irritation Potential of Tobacco Products

There is an increasing need by the tobacco industry to evaluate the irritation and inflammation potential of tobacco products to support product development goals, for sound product stewardship, and likely regulatory safety tests. The use of in vitro human cell and tissue-based test methods to replace in vivo animal models addresses the need for more […]