Skip to Main Content
+1 (508) 881-6771
0
Menu Button

Microbial infection

819

Azithromycin and Doxycycline Attenuation of Acanthamoeba Virulence in a Human Corneal Tissue Model

Background: Amoebic keratitis is a potentially blinding eye infection caused by ubiquitous, free-living, environmental acanthamoebae, which are known to harbor bacterial endosymbionts. A Chlamydia-like endosymbiont has previously enhanced Acanthamoeba virulence in vitro.We investigated the potential effect of Acanthamoeba-endosymbiont coinfection in a human corneal tissue model representing clinical amoebic keratitis infection. Methods: Environmental and corneal Acanthamoeba isolates from the […]
818

Shigella depends on SepA to destabilize the intestinal epithelial integrity via cofilin activation

Shigella is unique among enteric pathogens, as it invades colonic epithelia through the basolateral pole. Therefore, it has evolved the ability to breach the intestinal epithelial barrier to deploy an arsenal of effector proteins, which permits bacterial invasion and leads to a severe inflammatory response. However, the mechanisms used by Shigella to regulate epithelial barrier permeability remain unknown. To […]
677

BACTERIAL INACTIVATION OF WOUND INFECTION IN A HUMAN SKIN MODEL BY LIQUID-PHASE DISCHARGE PLASMA.

Background:  We investigate disinfection of a reconstructed human skin model contaminated with biofilm-formative Staphylococcus aureus employing plasma discharge in liquid. Principal Findings: We observed statistically significant 3.83-log10 (p,0.001) and 1.59-log10 (p,0.05) decreases in colony forming units of adherent S. aureus bacteria and 24 h S. aureus biofilm culture with plasma treatment. Plasma treatment was associated […]
311

HARVEST, PROLIFERATION, AND FUNCTIONAL TESTING OF HUMAN DENDRITIC CELLS.

Purpose: Difficulty in harvesting large numbers of cells, short survival time, and rapid phenotypic changes in culture have prevented the widespread use of human dendritic cells (DC) for many fundamental studies applicable to the initial stages of pharmaceutical discovery and development. The ability to overcome these problems would enable a broad variety of extremely useful […]