New Human Organotypic Corneal Tissue Model for Ophthalmic Drug Delivery Studies
PURPOSE. The purpose of the current work was to develop a physiologically relevant, in vitro human three-dimensional (3D) corneal epithelial tissue model for use in ophthalmic drug development. METHODS. Normal human corneal epithelial cells were cultured at the air–liquid interface to produce the 3D corneal tissue model. Corneal barrier was determined by measuring transepithelial electrical resistance (TEER). Quantitative PCR arrays […]