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Basic cutaneous research

815

In Vitro Skin Models and Their Predictability in Defining Normal and Disease Biology, Pharmacology, and Toxicity

In vitro skin model systems are increasingly being used both in the early evaluation of therapeutic drug candidates and in confirmatory mechanistic studies. The most commonly used of these in vitro model systems are reconstituted human epidermis (RHE) models. These RHE skin models consist solely of epidermal keratinocytes, which comes with some limitations but also with the advantage […]
802

Combined reflectance confocal microscopy/optical coherence tomography imaging for skin burn assessment

A combined high-resolution reflectance confocal microscopy (RCM)/optical coherence tomography (OCT) instrument for assessing skin burn gravity has been built and tested. This instrument allows for visualizing skin intracellular details with submicron resolution in the RCM mode and morphological and birefringence modifications to depths on the order of 1.2 mm in the OCT mode. Preliminary testing of […]
726

CELL TYPE-DEPENDENT GENE TRANSCRIPTION PROFILE IN A THREE-DIMENSIONAL HUMAN SKIN TISSUE MODEL EXPOSED TO LOW DOSES OF IONIZING RADIATION: IMPLICATIONS FOR MEDICAL EXPOSURES.

The concern over possible health risks from exposures to low doses of ionizing radiation has been driven largely by the increase in medical exposures, the routine implementation of X-ray backscatter devices for airport security screening, and, most recently, the nuclear incident in Japan. Because of a paucity of direct epidemiological data at very low doses, […]
764

INGENOL MEBUTATE INDUCED CELL DEATH PATTERNS IN NORMAL AND CANCER EPITHELIAL CELLS

We investigated the proposed necrotic mechanism of ingenol mebutate, a natural compound with anti-cancer properties in human keratinocytes, the human squamous cell carcinoma cell line HSC-5, and HeLa cervix carcinoma cells. Topical application of a clinical dose of ingenol mebutate 0.05% (1.15 mM) gel to human reconstituted full-thickness skin equivalents strongly reduced epidermal, but not […]
640

SIMULATION OF ELECTRON-BEAM IRRADIATION OF SKIN TISSUE MODEL.

Monte Carlo simulation of electrons stopping in liquid water was used to model the penetration and quality of electron-beam irradiation incident on the full-thickness EpiDermTM skin model (EpiDermFTTM Mattek, Ashland, VA). This 3D tissue model has a fully developed basement membrane separating an epidermal layer of keratinocytes in various stages of differentiation from a dermal […]
599

A NEW DECORIN-LIKE TETRAPEPTIDE FOR OPTIMAL ORGANIZATION OF COLLAGEN FIBRES.

Decorin interacts with collagen via its protein core and influences collagen fibrillogenesis, thus regulating excessive bundle-like aggregation of collagen. As skin ages, there is lack of functional decorin, which results in disrupted collagen fibres and in a reduction in the tensile strength of the skin. Therefore, a substitute for decorin would make up for the […]
563

RELATIVE BIOLOGICAL EFFECTIVENESS OF PULSED AND CONTINUOUS 20 MEV PROTONS FOR MICRONUCLEUS INDUCTION IN 3D HUMAN RECONSTRUCTED SKIN TISSUE.

Background and purpose: Laser accelerated radiotherapy is a prospect for cancer treatment with proton and/or carbon ion beams that is currently under fast development. In principal, ultra fast, high-energy laser pulses will lead to a ‘‘pulsed” delivery of the induced ion beam with pulse durations of 1 ns and below, whereas conventional proton beams deriving […]
530

SKIN SCIENCE: THE USE OF GENOMICS TOOLS IN SKIN CARE.

This study by scientists at Genemarkers LLC demonstrated that Mattek’s EpiDermFT full-thickness in vitro human skin tissue equivalent can be used to identify the human genes activated when anti-aging and anti-wrinkle cosmeceutical products are applied to the apical surface. Elina Ambra-Lift Activates Genes that Promote Anti-Aging and Anti-Wrinkle Effects. The use of genomics to validate […]
467

SYNERGISTIC ACTIONS OF PEMPHIGUS VULGARIS IGG, FAS-LIGAND AND TUMOR NECROSIS FACTOR-A DURING INDUCTION OF BASAL CELL SHRINKAGE AND ACANTHOLYSIS.

This study by researchers at the University of California at Davis demonstrated that when Mattek’s EpiDerm-FT epidermal/dermal human skin tissue equivalents were treated with pemphigus vulgaris (PV) IgGs, it resulted in suprabasilar epidermal clefting and the cell detachment pattern characteristic of the acantholysis seen in the skin of pemphigus vulgaris patients. This study by researchers […]
618

PROTEOMIC ANALYSIS OF LASER MICRODISSECTED MELANOMA CELLS FROM SKIN ORGAN CULTURES.

Gaining insights into the molecular events that govern the progression from melanoma in situ to advanced melanoma and understanding how the local microenvironment at the melanoma site influences this progression are two clinically pivotal aspects that to date are largely unexplored. In an effort to identify key regulators of the crosstalk between melanoma cells and […]