Normal Human Epidermal Keratinocytes

MatTek’s primary Normal Human Epidermal Keratinocytes (NHEK) and Growth Medium (NHEK-GM) provide an optimized platform for investigating keratinocyte biology.   Available from neonatal or adult donors across a variety of ethnicities, MatTek’s NHEKs offer quality and convenience for the study of inflammation, oxidative stress, toxicity & phototoxicity, wound healing, drug screening and tissue modeling.

Donor matched cell types (fibroblasts & melanocytes) are available upon request.

Normal Human Epidermal Keratinocytes (NHEK), 10xNormal Human Epidermal Keratinocytes (NHEK), 10x
NHEK-CRY-NEO – Cytokeratin 14 (green), Phalloidin (red), DAPI (blue), 60xNHEK-CRY-NEO – Cytokeratin 14 (green), Phalloidin (red), DAPI (blue), 60x
Normal Human Epidermal Keratinocytes (NHEK-CRY-NEO), 60xNormal Human Epidermal Keratinocytes (NHEK-CRY-NEO), 60x
NHEK-CRY-NEO – Cytokeratin 14 (green), Phalloidin (red), Ki67 (white), DAPI (blue), 180xNHEK-CRY-NEO – Cytokeratin 14 (green), Phalloidin (red), Ki67 (white), DAPI (blue), 180x
  • Product Description
  • Specifications
  • Pricing and Ordering

MatTek’s NHEKs are isolated from neonatal (NHEK-CRY-NEO) or adult (NHEK-CRY-AD) donor tissue and cryopreserved as primary cells.  When cultured in MatTek’s NHEK-GM Growth Medium according to MatTek’s NHEK-CRY protocols, our epidermal keratinocytes are guaranteed to have ≥ 75% viability upon thaw and to produce ≥ 15 population doublings.  NHEKs are characterized by their cobblestone morphology and expression of cytokeratins.

Primary Human Epidermal Keratinocytes cultured in NHEK-GM were stained for Keratin 14 (pink), Phalloidin (blue) and Ki67 (yellow)
Primary Human Epidermal Keratinocytes cultured in NHEK-GM were stained for Keratin 14 (pink), Phalloidin (blue) and Ki67 (yellow)

Download the Human Epidermal Keratinocytes Data Sheet

Download the NHEK Antioxidant Application Note

 

 

Specifications Table_NHEK

All cells are negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected by PCR for all donors and cell lots.  This product is for research use only.  Not for use in animals, humans or diagnostic purposes.

 

Get a Quote

MatTek’s cryopreserved human epidermal keratinocytes are shipped on dry ice every Monday, Tuesday, and Wednesday. To place an order, send a message or purchase order to sales@mattek.com or call customer service at +1-508-881-6771.

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