Highly-Multiplexed Immunohistochemistry Services
Biomarker profiling of tissues with 10+ markers on a single slide using our multiplex immunolabeling and imaging services
Modern medicine requires detailed understanding not only of the disease, but of the body’s response to it. A deeper understanding of cellular interactions within the context of cancer and inflammatory diseases is critical to developing better treatments and therapeutic outcomes. With advances in fluorescent probes and imaging technologies, fluorescent immunohistochemistry is a powerful tool to probe cell populations and protein distribution within tissues, but are fundamentally limited by the number of markers that can be assessed simultaneously.
Traditionally, researchers have been limited to the number of markers they can use simultaneously when characterizing slides due to the inherent limitations of fluorescent or chromogenic labeling. At Visikol, we provide our Clients with the ability to move beyond traditional slide labeling and imaging such that they can achieve 10+ labeling from a single slide. We accomplish this using our proprietary EasyPlex™ antibody stripping reagent and co-registration software that allows for antibodies to be removed from tissues such that multiple rounds of sequential labeling can be conducted from the same slide.
Rapid, inexpensive, high-plex microenvironment and biomarker profiling of tissue with any antibody.
Cutting-Edge Tools
Using our proprietary EasyPlex™ antibody stripping reagent, multiple rounds of immunolabeling can be conducted on a single slide, providing unprecedented detail about your samples.
High Value Endpoints
Using digital slide imaging and advanced image analysis, a large number of valuable endpoints can be captured from a single specimen, giving unique insights about cell populations and interactions.
Take Action
Our expert team processes images and analyzes data, delivering actionable insights in an easy-to-read report, so you can make decisions and push forward your research efforts.
How It Works
Send Your Tissues
Send us your fixed tissue samples, FFPE tissue blocks, or unstained slides.
Choose Your Panels
Select the markers for your specific research needs. You can choose up to 15 markers.
Multiplex Fluorescent Immunohistochemistry
Our team of experts labels your samples with 3-4 antibodies in each round of labeling, plus a nuclear counterstain. Using fluorescent slide scanning we image your labeled samples at 10x, 20x, or 40x magnification.
Antibody Stripping with EasyPlex™
Using our proprietary EasyPlex™ technology, we strip all antibody probes from the tissue and return it to its original state.
Multiple Rounds of Labeling
Multiple rounds of labeling, imaging, and stripping are conducted, allowing for extremely detailed profiling of the cell population within your tissue sample. H&E staining is the final step if desired.
Imaging and Co-registration
The imaging data acquired from multiple rounds of labeling is co-registered using custom-built image processing algorithms to create a highly-multiplexed fluorescence image stack.
Endpoints
Quantitative analysis of image data allows for the measurement of cell populations at unprecedented detail within a single sample. Our advanced image analysis algorithms allow for evaluation of the spatial distribution of cells within regions of interest within your samples.