EpiAlveolar cell types

EpiAlveolar tissues are produced on cell culture inserts by differentiating primary alveolar epithelial cells, pulmonary endothelial cells and fibroblasts using a series of proprietary media. Differentiated EpiAlveolar tissues have a squamous epithelium consisting of Type I and Type II alveolar epithelial cells cultured with extracellular matrix producing pulmonary fibroblasts and endothelial cells. The epithelium exhibits functional tight junctions and a robust barrier that remains stable for studies lasting more than 30 days.

Top view confocal image of EpiAlveolar. Tight junction marker Occludin (green) and nuclei marker DAPI (blue)

EpiAlveolar TEER

                       EpiAlveolar co-culture with THP-1 macrophages, (ALV-100-FT-MAC-PE12)

Cross-sectional confocal image. Epithelial cell marker cytokeratin 19 (green), fibroblast marker Vimentin (red) and nuclei marker DAPI (blue)

The EpiAirway 3D human tissue model is routinely utilized for a range of applications including safety & risk assessment, inflammation & fibrosis, and drug delivery. Simple protocols and the evaluation of early cellular endpoints allow scientists to acquire data in days, not weeks or months.

Inhalation Toxicology

Use EpiAlveolar to determine relative safety and toxicological potential of inhaled substances.

Technical Reference 845

Technical Reference 758

Disease Modeling

Use of EpiAlveolar Lung Model to Predict Fibrotic Potential of Multiwalled Carbon Nanotubes

Hana BarosovaAnna G. MaioneDedy SeptiadiMonita SharmaLaetitia HaeniSandor BalogOlivia O’ConnellGeorge R. JacksonDavid BrownAmy J. ClippingerPatrick HaydenAlke Petri-FinkVicki Stone, and Barbara Rothen-Rutishauser. ACS Nano Article ASAP. DOI: 10.1021/acsnano.9b06860

Technical Reference 844

Technical Reference 845

Drug Development

Technical Reference 758

Host-Pathogen Interaction

Tissue

Kit: A standard EpiAlveolar kit (ALV-100-FT-PE12) consists of 24 tissues. (Tissue “kits” contain tissues, 250 mL of culture medium, and plasticware; contact MatTek for specific kit contents). ALV-100-FT-MAC-PE12, containing THP-1 macrophages is also available.

Formats: 12mm Transwell inserts – tissue culture substrate is chemically modified with a pore size of 0.4 μm

Culture: Air-liquid interface

Histology: 1-2 cell layers – highly squamous morphology and tissue structure.

Lot numbers: Tissue lots produced each week are assigned a specific lot number. A letter of the alphabet is appended to the end of the lot number to differentiate between individual kits within a given lot of tissues. All tissue kits within a lot are identical in regards to cells, medium, handling, culture conditions, etc.

Shipment: At 4°C on medium-supplemented, agarose gels

Shipment Day: ALV-100-FT-PE12 – Every Monday. ALV-100-FT-MAC-PE12 – Every Tuesday.

Delivery: ALV-100-FT-PE12 – Tuesday morning via FedEx priority service (US). Outside US: Tuesday-Wednesday depending on location. ALV-100-FT-MAC-PE12 – Wednesday morning via FedEx priority service (US). Outside US: Wednesday-Thursday depending on location.

Shelf Life: Including time in transit, tissues may be stored at 4°C for up to 3 days prior to use. However, extended storage periods are not recommended. In addition, the best reproducibility will be obtained if tissues are used consistently on the same day.

Length of Experiments: Tissue cultures can be continued for TWO (2) WEEKS or more with good retention of normal morphology. Tissues must be fed every other day with 5.0 ml of maintenance medium (ALV-100-MM). Cell culture inserts are placed into 6-well hanging culture plates provided (HNG-TOP-6-22).

Cells

Type: Normal human alveolar epithelial cells (NHAE); Normal human pulmonary fibroblasts (NHPF), Normal human pulmonary endothelial cells (NHPE) and THP-1 macrophages (ALV-100-FT-MAC-PE12).

Genetic make-up: Multiple donors

Derived from: Healthy, non-smoker

Screened for: HIV, Hepatitis-B, Hepatitis-C, mycoplasma

Medium

Base medium: Proprietary

Growth factors/hormones: Epidermal growth factors and other proprietary factors

Antibiotics: Gentamicin 30 µg/ml

Anti-fungal agent: Amphotericin B 15 ng/ml

pH Indicator: Phenol red

Other additives: Proprietary

Maintenance medium: ALV-100-MM

Quality Control and Sterility

Visual inspection: All tissues are visually inspected and if physical imperfections are noted, tissues are rejected for shipment

End-use testing: Transepithelial electrical resistance (TEER) of each EpiAlveolar lot is measured using an EVOM2 epithelial Volthommeter and Endohm electrode chamber (World Precision Instruments).

Sterility: All media used throughout the production process is checked for sterility. Maintenance medium is incubated without antibiotics for 1 week and checked for sterility. The agarose gel from the 24-well plate used for shipping is also incubated for 1 week and checked for any sign of contamination

Screening for pathogens: All cells are screened and are negative for HIV, hepatitis B and hepatitis C using PCR. However, no known test method can offer complete assurance that the cells are pathogen-free. Thus, these products and all human-derived products should be handled at BSL-2 levels (biosafety level 2) or higher as recommended in the CDC-NIH manual, “Biosafety in microbiological and biomedical laboratories,” 1998. For further assistance, please contact your site Safety Officer or MatTek technical service

Notification of lot failure: If a tissue lot fails our QC or sterility testing, the customer will be notified and the tissues will be replaced without charge when appropriate. Because our QC and sterility testing is done post-shipment, a notification will be made as soon as possible (Under normal circumstances, TEER failures will be notified by Wednesday 5 p.m.; sterility failures will be notified within 8 days of shipment)

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