EpiCorneal™

The EpiCorneal 3D human tissue model provides a highly predictive non-animal alternative to assess drug delivery, infection, wound healing/tissue regeneration and disease modeling (i.e. Dry Eye).

Corneal

MatTek’s EpiCorneal tissue model consists of normal human corneal epithelial cells cultured to form a stratified, squamous epithelium which closely parallels normal human corneal tissue. The corneal cells, cultured on specially prepared cell culture inserts with serum-free medium, differentiate to form a multi-layered structure.

Ultrastructural features of EpiCorneal. Apical cell layer of reconstructed tissue. Open arrows point to microvilli on the surface of the tissue. Tight junctions (white arrows) and desmosomes (closed arrow) are observed at the junction of adjacent epithelial cells.
TEM Analysis of EpiCorneal illustrates the presence of 1) microvilli, 2) tight junctions (white arrows), and 3) desmosomes (black arrows)
Confocal for Homepage
Tight Junction Protein ZO-1 (green)

Used in drug delivery and drug development, EpiCorneal is a mitotically and metabolically active tissue with barrier properties approaching those of the human cornea. EpiCorneal can be stimulated to release many cytokines known to be important in inflammation and diseases such as dry eye. Further, the advanced 3D tissue model expresses cornea specific drug transporters and metabolizing enzymes.

Barrier Properties of EpiCorneal compared to EpiOcular measured by transepithelial electrical resistance (TEER).
Gene Expression Analysis shows the expression of cornea specific markers, drug metabolizing enzymes and transporters including Aldehyde Dehydrogenase (ALDH3A1), Pyrimidine Nucleotide Oxidoreductase (TRXR1) and Mucin 4 (MUC4)

Corneal Drug Delivery and Corneal Toxicity

EpiCorneal closely recapitulates the physiology, 3D tissue architecture, and function of the human cornea for use in pharmaceutical development applications.

Contact Dr. Yulia Kaluzhny for more information.

Browse our reference library to see how our EpiCorneal tissue has been used in these areas of study.

Tissue

Kit: A standard EpiCorneal kit (COR-100) consists of 24 tissues. (Tissue “kits” contain tissues, a small amount of culture medium, and plasticware; contact MatTek for specific kit contents)

Formats: 9mm individual inserts – tissue culture substrate is chemically modified with a pore size of 0.4 μm

Culture: Air-liquid interface

Histology: 4-5 cell layers – stratified, squamous morphology and tissue structure

Lot numbers: Tissue lots produced each week are assigned a specific lot number. A letter of the alphabet is appended to the end of the lot number to differentiate between individual kits within a given lot of tissues. All tissue kits within a lot are identical in regards to cells, medium, handling, culture conditions, etc.

Shipment: At 4°C on medium-supplemented, agarose gels

Shipment day: Every Monday

Delivery: Tuesday morning via FedEx priority service (US). Outside US: Tuesday-Thursday depending on location

Shelf life: Including time in transit, tissues may be stored at 4°C for up to 3 days prior to use. However, extended storage periods are not recommended unless necessary. In addition, the best reproducibility will be obtained if tissues are used consistently on the same day, e.g. Tuesday afternoon or following overnight storage at 4°C (Wednesday morning)

Cells

Type: Normal human corneal epithelial cells (HCEC)

Genetic make-up: Single donor

Derived from: Healthy donor

Screened for: HIV, Hepatitis-B, Hepatitis-C, mycoplasma

Quality Control and Sterility

Visual inspection: All tissues are visually inspected and if physical imperfections are noted, tissues are rejected for shipment

Sterility: All media used throughout the production process is checked for sterility. Maintenance medium is incubated with and without antibiotics for 1 week and checked for sterility. The agarose gel from the 24-well plate used for shipping is also incubated for 1 week and checked for any sign of contamination

Screening for pathogens: All cells are screened and are negative for HIV, hepatitis B and hepatitis C using PCR. However, no known test method can offer complete assurance that the cells are pathogen free. Thus, these products and all human derived products should be handled at BSL-2 levels (biosafety level 2) or higher as recommended in the CDC-NIH manual, “Biosafety in microbiological and biomedical laboratories,” 1998. For further assistance, please contact your site Safety Officer or MatTek technical service

Notification of lot failure: If a tissue lot fails our QC or sterility testing, the customer will be notified and the tissues will be replaced without charge when appropriate. Because our QC and sterility testing is done post-shipment, notification will be made as soon as possible (Under normal circumstances, ET-50 failures will be notified by Wednesday 5 p.m.; sterility failures will be notified within 8 days of shipment)

New Orders

To ensure that the most appropriate products and accessories for your application are included with your initial order, MatTek strongly recommends a complimentary consultation with one of our highly trained Technical Specialists. Please contact Dr. Yulia Kaluzhny or Viktor Karetsky to order EpiCorneal products.

Yulia Kaluzhny, Ph.D.
MatTek Corporation
ykaluzhny@mattek.com
T: (508) 881-6771 Ext. 229
F: (508) 879-1532

Viktor Karetsky
MatTek Corporation
vkaretsky@mattek.com
T: (508) 881-6771 Ext. 337
F: (508) 879-1532

All Other Orders

Please contact Customer Service via telephone or fax.

Customer Service
MatTek Corporation
T: (508) 881-6771
F: (508)-879-1532

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Cost-Effective & Reproducible

All MatTek tissue models are produced to the highest quality.

  • UNSURPASSED LONG-TERM TISSUE REPRODUCIBILITY LOT-TO-LOT, YEAR-TO-YEAR
  • 3-DIMENTIONAL, HIGHLY DIFFERENTIATED TISSUE
  • METABOLICALLY, MITOTICALLY ACTIVE