THE USE OF STANDARDIZED HUMAN SKIN MODELS FOR CUTANEOUS PHARMACOTOXICOLOGY STUDIES.

Reconstructed skin and epidermis models are finding increasingly numerous applications in cosmetology and dermatology. In particular, they are currently employed to assess the tolerability and efficacy of raw materials and formulations, in conditions approaching those of normal use. Importantly, the use of such models greatly reduces the need for animal testing. Various models of reconstructed skin and epidermis have been developed [1-4] and some are now produced industrially [5-8]. They are used for the prediction of cutaneous irritancy and, to a lesser extent, percutaneous absorption and cutaneous metabolism. However, before being officially recognized as valid alternative methods and entering routine use, standard protocols must be developed to assess their reproducibility and performance as compared with in situ human studies.

Benzoic acid, Caffeine, Cutaneous absorption, Cutaneous irritancy, Cutaneous irritation, Cutaneous metabolism, Cutaneous toxicity, Cytochrome P450, Dermal irritancy, Dermal irritation, EpiDerm, EpiDermal differentiation, EpiSkin, Epidermis models, GOT, Glutathione S-transferase, Human skin models, IL-1a, In vitro testing, LDH, MTT, MTT ET-50 tissue viability assay, MTT assay, Mannitol, Metabolism, NAD(P)H, P 450, Percutaneous absorption, Permeation, Pharmacotoxicology, Reconstructed epidermis, Reconstructed human epidermis, Reconstructed skin, Reproducibility, Skin irritancy, Skin irritation

Benzoic acid, Caffeine, Mannitol