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ASSESSMENT OF PHOTOTOXICITY, SKIN IRRITATION, AND SENSITIZATION POTENTIAL OF POLYSTYRENE AND TiO2 NANOPARTICLES.

Park1, Y-H. , Jeong1, S.H., Yi1, S.M., Choi1, B.H., Kim2, Y-R., Kim2, I-K., Kim2, M-K., and Son1, S.W.  1Laboratory of Cell Signaling and Nanomedicine, Department of Dermatology and Division of Brain Korea 21 Project for Biomedical Science, Korea University College of Medicine, Seoul, Korea. 2Department of Biochemistry & Molecular Biology, Korea University College of Medicine, Seoul, Korea.
Abstract

The human skin equivalent model (HSEM) is well known as an attractive alternative model for evaluation of dermal toxicity. However, only limited data are available on the usefulness of an HSEM for nanotoxicity testing. This study was designed to investigate cutaneous toxicity of polystyrene and TiO2 nanoparticles using cultured keratinocytes, an HSEM, and an animal model. In addition, we also evaluated the skin sensitization potential of nanoparticles using a local lymph node assay with incorporation of BrdU. Findings from the present study indicate that polystyrene and TiO2 nanoparticles do not induce phototoxicity, acute cutaneous irritation, or skin sensitization. Results from evaluation of the HSEMs correspond well with those from animal models. Our findings suggest that the HSEM might be a useful alternative model for evaluation of dermal nanotoxicity.

Keywords

EPI-200-PHO, EPI-200-SIT, EpiDerm Phototoxicity Test, EpiDerm Skin Irritation Test, nanoparticles, phototoxicity, skin irritation

Materials Tested

Polystyrene latex beads (amine-modified 50nm), titanium dioxide TiO2

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