AN IN VITRO MODEL SYSTEM TO STUDY THE DAMAGING EFFECTS OF PROLONGED MECHANICAL LOADING OF THE EPIDERMIS.

This study by scientists at the Eindhoven University of Technology demonstrated that MatTek’s EpiDerm human skin tissue equivalent can be used to improve insight in the epidermal damage process due to prolonged mechanical loading, and can serve as a sound basis for effective clinical identification and prevention of pressure ulcers. Pressure ulcers are areas of soft tissue breakdown that result from sustained mechanical loading of the skin and underlying tissues. Today, little is known with respect to the aetiology of these ulcers. This study by researchers in the Dept. of Biomedical Engineering at the Eindhoven University of Technology introduces EpiDerm™ an in vitro model system to study the effects of clinically relevant loading regimes on damage progression in the epidermis, the uppermost skin layer. Engineered epidermal equivalents (EpiDerm) were subjected to 6.7 and 13.3 kPa for either 2 or 20 h using a custom-built loading device. Tissue damage was assessed by (1) histological examination, (2) tissue viability evaluation, and (3) by the release of a pro-inflammatory mediator, interleukin-1á (IL-1á). Loading the EpiDerm samples for 2 h increased the IL-1á release, although no visible tissue damage was observed. However, in the 20 h loading experiments visible tissue damage and a small decrease in tissue viability were observed. Furthermore, in these experiments, the IL-1á release increased with magnitude of loading. It is concluded that this (EpiDerm) in vitro model system can be applied to improve insight in the epidermal damage process due to prolonged mechanical loading, and can serve as a sound basis for effective clinical identification and prevention of pressure ulcers.

Cell swelling, Cytoplasmic swelling, EPI-200, EpiDerm, Histology, IL-1a, IL-1a Batch variation, Interleukin 1a (IL-1a), MTT, Mechanical Loading, Mechanically induced skin damage, Necrosis, Pressure ulcers, Tissue viability, Ulcers