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USE OF DIFFERENTIATED EPIDERMAL CULTURES FOR THE EXAMINATION OF INFANT-FECES-INDUCED SKIN IRRITATION.

Hendrix, S.W. Procter and Gamble, Cincinnati, Ohio.
Abstract

Infant feces (BM) is known to possess a multitude of skin irritants. The aim of this study was to examine an in vitro model for BM-induced skin irritation, and determine if BM-induced skin irritation can be patterned in the laboratory. Infant BM was examined by diluting in water as individual samples or by pooling samples for topical application to MatTek EPI-200 hydrocortisone free, human epidermal cultures. Over a series of experiments IL-lα, IL-8, and PGE-2 were examined as pro-inflammatory endpoints, and the reduction of MTT was employed as a cytotoxicity endpoint. Infant BM is not cytotoxic to the in vitro model and infant BM causes the production and release of pro-inflammatory mediators. Infant BM-induced skin irritation is varied between individual samples of BM, and skin culture responses to infant BM diminish with lowering the concentration of infant BM topically applied.

Keywords

BM, Cutaneous irritancy, Cutaneous irritation, Cutaneous toxicity, Dermal irritancy, Dermal irritancy testing, Dermal irritation, EpiDerm, IL-1a, IL-8, Infant feces-induced skin irritation, Irritation (skin), MTT, MTT ET-50 tissue viability assay, MTT assay, PGE-2, Pro-inflammatory, Prostaglandin E-2, Skin irritancy, Skin irritation

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