821

Primary human polarized small intestinal epithelial barriers respond differently to a hazardous and an innocuous protein

A.D. Eaton, C. Zimmermann, B. Delaney, B.P. Hurley
Abstract

An experimental platform employing human-derived intestinal epithelial cell (IEC) line monolayers grown on permeable Transwell® filters was previously investigated to differentiate between hazardous and innocuous proteins. This approach was effective at distinguishing these types of proteins and perturbation of monolayer integrity, particularly transepithelial electrical resistance (TEER), was the most sensitive indicator. In the current report, in vitro indicators of monolayer integrity, cytotoxicity, and inflammation were evaluated using primary (non-transformed) human polarized small intestinal epithelial barriers cultured on Transwell® filters to compare effects of a hazardous protein (Clostridium difficile Toxin A [ToxA]) and an innocuous protein (bovine serum albumin [BSA]). ToxA exerted a reproducible decrease on barrier integrity at doses comparable to those producing effects observed from cell line-derived IEC monolayers, with TEER being the most sensitive indicator. In contrast, BSA, tested at concentrations substantially higher than ToxA, did not cause changes in any of the tested variables. These results demonstrate a similarity in response to certain proteins between cell line-derived polarized IEC models and a primary human polarized small intestinal epithelial barrier model, thereby reinforcing the potential usefulness of cell line-derived polarized IECs as a valid experimental platform to differentiate between hazardous and non-hazardous proteins.

Share on FacebookTweet about this on TwitterShare on LinkedInEmail this to someone
Keywords

EpiIntestinal (SMI-100), food toxicity, hazardous proteins, barrier integrity, inflammation, Inulin flux, LDH, horseradish peroxidase (HRP) Flux Assay, MTT assay, TEER, IL-6, IL-8

Materials Tested

Clostridium difficile Toxin A, bovine serum albumin, tumor necrosis factor-alpha, Flagellin, Inulin, triton X-100

Request a copy of this paper, click here.