Modulation of cutaneous scavenger receptor B1 levels by exogenous stressors impairs “in vitro” wound closure

Ximena Maria Muresana, Claudia Sticozzia, Giuseppe Belmontea, Vinno Savellic, Pablo Evelsond, Giuseppe Valacchia

Scavenger receptor B1 (SR-B1) is a trans-membrane protein, involved in tissue reverse cholesterol transport. Several studies have demonstrated that SR-B1 is also implicated in other physiological processes, such as bacteria and apoptotic cells recognition and regulation of intracellular tocopherol and carotenoids levels. Among the tissues where it is localized, SR-B1 has been shown to be significantly expressed in human epidermis. Our group has demonstrated that SR-B1 levels are down-regulated in human cultured keratinocytes by environmental stressors, such as cigarette smoke, via cellular redox imbalance. Our present study aimed to investigate whether such down-regulation was confirmed in a 3D skin model and under other environmental challengers such as particulate matter and ozone. We also investigated the association between oxidation-induced SR-B1 modulation and impaired wound closure. The data obtained showed that not only cigarette, but also the other environmental stressors reduced SR-B1 expression in epidermal cutaneous tissues and that this effect might be involved in impaired wound healing.


EpiDerm (EPI-200), Scavenger receptor B1, EPI-200, Oxidative stress, Pollution, Wound healing, Particulate matter, Cigarette smoke, Ozone, protein carbonyls, protein oxidation

Materials Tested

concentrated air particles, ozone, cigarette smoke

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