LIPID CHARACTERIZATION OF A NEW EPIDERMAL MODEL FOR SKIN IRRITANCY AND PENETRATION STUDIES.
A model of human epidermis, EpiDerm™, based on neonatal, foreskin-derived normal human epidermal keratinocytes (NHEK) has recently been characterized by scientists at the University of Iowa College of Dentistry in terms of stratum corneum lipids, ultrastructure, and barrier properties. Cultured using serum free media, the EpiDerm model allows determination of the effects of defined media additives on stratum corneum lipids, intercellular lamellae and epidermal barrier properties. Quantitative thin layer chromatography shows lipid profiles remarkably close to that of native epidermis including the linoleate rich acyl-ceramide (Ceramide 1) which has been strongly implicated in maintenance of normal epidermal barrier function. RuO4 stained thin sections observed using transmission electron microscopy show an alternating broad-narrow-broad pattern, characteristic of the intercellular lamellae of normal epidermal stratum corneum. These results, along with others showing normal epidermal structure, biochemistry, and function, provide the theoretical basis for use of the EpiDerm model to study dermal irritation and skin penetration in vitro.
Barrier properties formation, Ceramides, Cutaneous irritancy, Cutaneous irritation, Cutaneous toxicity, Dermal irritancy, Dermal irritancy testing, Dermal irritation, Dermal penetration, Electron microscopy, transmission (TEM), EpiDerm, Lipids, ceramides, Percutaneous absorption, Profiles/Analyses, Skin irritancy, Skin irritation, Ultrastructural Characteristics of
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