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IN VITRO DERIVED DENDRITIC CELLS TRANS-INFECT CD4 T CELLS PRIMARILY WITH SURFACE-BOUND HIV-1 VIRIONS.

Cavrois1, M., Neidleman1, J., Kreisberg1,2, J.F., Greene1,2,3, W.C. 1Gladstone Institute of Virology and Immunology, University of California San Francisco, San Francisco, California, United States of America, 2Department of Medicine, University of California San Francisco, San Francisco, California, United States of America, 3Department of Microbiology and Immunology, University of California San Francisco, San Francisco, California, United States of America.
Abstract

This study by researchers at the University of California at San Francisco (UCSF) demonstrated that MatTek’s Human Dendritic Cells can be used to study the infection mechanism of HIV-1 virus from dendritic cells to T cells. In the prevailing model of HIV-1 trans-infection, dendritic cells (DCs) capture and internalize intact virions and transfer these virions to interacting T cells at the virological synapse. In this study, researchers show that HIV-1 virions transmitted from in vitro derived dendritic cells to T cells principally originate from the surface of DCs. Selective neutralization of surface-bound virions abrogated trans-infection by monocyte-derived DCs and CD34-derived Langerhans cells (Human Dendritic Cells, MatTek Corp.). Under conditions mimicking antigen recognition by the interacting T cells, most transferred virions still derived from the cell surface, although a few were transferred from an internal compartment. UCSF study findings suggest that attachment inhibitors could neutralize trans-infection of T cells by DCs in vivo.

Keywords

Antigen recognition, Attachment inhibitors, CD34-derived Langerhans cells, Cell surface, Dendritic Cells, HIV-1 trans-infection, HIV-1 virions, In vitro derived DCs, Intact virions, Interacting T cells, Internal compartment, Monocyte-derived DCs, Surface-bound virions, Trans-infection, Virological synapse

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