IN VITRO DERIVED DENDRITIC CELLS TRANS-INFECT CD4 T CELLS PRIMARILY WITH SURFACE-BOUND HIV-1 VIRIONS.
This study by researchers at the University of California at San Francisco (UCSF) demonstrated that MatTek’s Human Dendritic Cells can be used to study the infection mechanism of HIV-1 virus from dendritic cells to T cells. In the prevailing model of HIV-1 trans-infection, dendritic cells (DCs) capture and internalize intact virions and transfer these virions to interacting T cells at the virological synapse. In this study, researchers show that HIV-1 virions transmitted from in vitro derived dendritic cells to T cells principally originate from the surface of DCs. Selective neutralization of surface-bound virions abrogated trans-infection by monocyte-derived DCs and CD34-derived Langerhans cells (Human Dendritic Cells, MatTek Corp.). Under conditions mimicking antigen recognition by the interacting T cells, most transferred virions still derived from the cell surface, although a few were transferred from an internal compartment. UCSF study findings suggest that attachment inhibitors could neutralize trans-infection of T cells by DCs in vivo.
Antigen recognition, Attachment inhibitors, CD34-derived Langerhans cells, Cell surface, Dendritic Cells, HIV-1 trans-infection, HIV-1 virions, In vitro derived DCs, Intact virions, Interacting T cells, Internal compartment, Monocyte-derived DCs, Surface-bound virions, Trans-infection, Virological synapse
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