IN HUMAN EPIDERMIS, BETA-DEFENSIN 2 IS PACKAGED IN LAMELLAR BODIES.
The skin presents a mechanical, as well as an immunological barrier to infection, and displays considerable innate immune capacity. Recently, cultured human keratinocytes were described to produce and export a microbicidal peptide human-defensin 2 (HBD-2). Immunogold was used to label ultrathin cryosections of stimulated, cultured human epidermis. HBD-2 was found to be stored in the lamellar bodies (LBs) of the stimulated keratinocytes of the spinous layer of the epidermis. HBD-2 was also found in the intercellular space. These findings suggest that HBD-2 is released with the contents of the LBs. Along with other investigations, our findings indicate that the lipid “permeability” barrier of the skin contains antimicrobial substances.
Air-liquid interface, Anti-microbial, Anti-microbial activity, Anti-microbial peptide, Anti-microbial peptides, Antimicrobial, Antimicrobial activity, Antimicrobial peptide, Antimicrobial peptides, Cryosection, Defensins, Electron microscopy, Electron microscopy (EM)-grade paraformaldehyde, Epi-200-3S, EpiDerm, HBD-2, Human beta-defensin 2, Immunogold, Interleukin 1a (IL-1a), Keratinocyte, Lamellar bodies, Lipid permeability barrier, Polyclonial rabbit anti-HBD-2 antibodies, Ultracryomicrotomy, Ultrathin cryosections
EPI-200-3S, Electron microscopy (EM)-grade paraformaldehyde, Interleukin (IL-1a)
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