Objective: The objective of this experiment was to determine whether the results previously obtained DC Detoxdefense in enzymatic and cell-based assays are corroborated by modulation of gene expression in EpiDerm human skin substitute model. Methods: EpiDerm tissues were obtained from Mattek (Ashland, MA) and cultured according to the manufacturer’s instructions. The test materials – Upregulex (Lot# F01071516) and DC Detoxdefense (Product # RON5-7/2, batch# H010705072) – were received on the 2nd of July 2007 and 15th of August, 2007, respectively. DC Detoxdefense at 0.5% (vol:vol). The incubation time with skin tissues was 3 days. After incubation, skin tissues were harvested, frozen in liquid nitrogen and subjected to RNA extraction with Qiagen kit. The quality of extracted RNA was assayed twice by electrophoresis (after extraction and before microarray analysis) and was determined to be better than control. Samples were hybridized on Affymetrix chip containing the whole human genome (library HT_HG-U133A). The resulting CEL files were then converted in CHP format using Expression Console software, which were then unlocked in Excel, yielding data on over 22,200 probes (see attached file “Upregulex & DC Detoxdefense DNA microarray profile SBD”). All probes, whose expression changed by 15% or more were flagged. Results and Discussion: The test was successful in terms that the quality of RNA was outstanding and that the treatment with test materials triggered variation in gene expression pattern in the skin tissue. Upregulex triggered 15% or greater change in the expression of 1087 probes. DC Detoxdefense did it for 1050 probes.


Anti-aging skin, Antioxidant enzyme (SOD) Defense, Cytochrome P450, Detoxification, Detoxifying, Energizing, Fungus, Laminin, MMP8, Mushrooms, Phospholipids vesicle, Pollution protection, SOD, Sun care, superoxide dismutase 2

Materials Tested

Beta vulgaris (beet) root extract, Butylenes glycol, Cordyceps, DC Detoxdefense, Phospholipids, Sinensis extract, Sugar beet extract

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