CONFOCAL MICROSCOPY FOR MODELING ELECTRON MICROBEAM IRRADIATION OF SKIN.
For radiation exposures employing targeted sources such as particle microbeams, the deposition of energy and dose will depend on the spatial heterogeneity of the sample. Although cell structural variations are rela- tively minor for two-dimensional cell cultures, they can vary significantly for fully differentiated tissues. Employ- ing high-resolution confocal microscopy, we have deter- mined the spatial distribution, size, and shape of epidermal keratinocyte nuclei for the full-thickness EpiDermTM skin model (MatTek, Ashland, VA). Application of these data to calculate the microdosimetry and microdistribution of energy deposition by an electron microbeam is discussed.
Confocal microscopy, DAPI, EFT-400, Electron microbeam, EpidermTM full thickness (FT), Irradiation, Keratin 10, Microbeam, Microdosimetry, Nuclear radius, Nuclear volume, Nucleic acid stains, Nucleic acids, Particle microbeams, SYTO13, SYTO59, Vimentin
Electron microbeams, Microbeams, Particle microbeams
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