ANTIOXIDANT CAPACITY OF 3D HUMAN SKIN EPIDERM MODEL: EFFECTS ON SKIN MOISTURIZERS.

This study by researchers at North Dakota State University, the University of North Dakota and the Plastic Surgery Institute demonstrated how MatTek’s EpiDerm in vitro 3-D human skin tissue equivalent can be used to better understand the mechanisms of the effects of skin moisturizers containing antioxidant ingredients on skin function at the tissue level, and to establish effective strategies for skin protection and clinical treatments of skin disorders, and possibly healing wounds. The objective of this study was to delertmine the effects of skin moisturizers on total antioxidant capacity (TAC) of human skin using the EpiDerm™ model. Three different skin moisturizers containing antioxidant ingredients (samples 1-3) or aloe vera extract were topically applied to EpiDerm tissues and incubated for 2 and 24 h to determine acute and longer-term effects of applied samples on total antioxidant capacity and glutathione peroxidase activity in medium and/or homogenized skin tissues. Total antioxidant capacity in medium and skin homogenates was enhanced (P < 0.0001) by gel containing antioxidant ingredients (sample 2) after 2 and 24 h of incubation. Total antioxidant capacity in medium was also enhanced (P < 0.0001) by cream containing antioxidant ingredients (sample 3) after 24 h of incubation. Overall, total antioxidant capacity in medium was greater (P < 0.02) after 24 h than 2 h of incubation. Skin moisturizer cream with high antioxidant levels determined by using oxygen radical absorbance capacity testing (sample 1) and aloe vera extract did not affect total antioxidant capacity. Glutathione peroxidase activity was enhanced (P < 0.0001) in medium and skin homogenates by sample 2 but not by any other sample. These data demonstrate high potential of gel and cream (samples 2 and 3) containing antioxidant ingredients in enhancing antioxidant capacity of EpiDerm tissues which will likely contribute to overall skin health. Results of this experiment will help to better understand mechanisms of effects of skin moisturizers containing antioxidant ingredients on skin function at the tissue level and to establish effective strategies for skin protection and clinical treatments of skin disorders and possibly healing wounds.

Antioxidant capacity, EPI-200, EpiDerm, Glutathione peroxidase, Moisturizer gel, Oxygen radical absorbance capacity, Skin moisturizers, TAC, Total antioxidant capacity, Trolox-equivalent capacity assay

Aloe vera, E. soy isoflavones, Lexli moisturizing gel, Pomegranate extract, Vitamin C