PermaCell Culture Inserts

MatTek PermaCell Culture Inserts are sterile, tissue culture devices that are ideal for the growth/differentiation of cells and 3D organotypic culture. The 8mm inserts are uniquely designed to function as either standing or hanging inserts, allowing researchers increased flexibility when designing studies. The inserts are sold in 24-well plates (24 inserts/plate), but can be used in 6-, 12-, and 24-well culture plate formats. Various membrane types allow researchers to select inserts for specific needs, such as cell attachment, differentiation, organotypic 3D culture, drug transport/permeability, and imaging studies.

For higher throughput applications, PermaCell 96-well insert plates are connected in a single, rigid plate that is compatible with robotic systems.

Both the individual inserts and the 96-well insert plates are specially designed for 3D organotypic tissue culture and are easily adaptable to TEER measurements and other analytical techniques.

Download the PermaCell Spec Sheet

8mm DOWNLOAD

96-well Insert Plate DOWNLOAD

Download the PermaCell Application Note

8mm DOWNLOAD

8mm Clear PTFE Inserts DOWNLOAD

96-well Insert Plate DOWNLOAD

Description

8mm Inserts

• 24 8mm inserts packaged in 24-well plate
• Hang-top lid for airlifting can be purchased separately for use in 12- or 24-well formats
• Pore size of 0.4µm or 8µm, custom pore sizes available
• PTFE, PET, PC membrane, and custom membranes available
  • PTFE membranes have a high pore density and are clear in culture, which allows for visualization of cells via brightfield and fluorescent microscopy
• TEER compatible

96-well Insert Plates

• 96-well insert plates connected in a single, rigid tray
• Pore size of 0.4µm
• PET high pore density membrane
• Robotics and TEER compatible
• Large access ports to allow for efficient media sampling
• System includes 96-well insert plate, 96-well reservoir plate, and lid
• Common (single) well reservoir plate may be purchased separately

Compatible TEER Electrode: STX HTS EVOM™ Electrode for TEER in PermaCell Culture Insert Plate

Need an EVOM² Adapter? STX4 Electrode Adapter Cable to Use with EVOM2

Specifications

8mm Insert Specifications
Height (including feet)	8.8 mm
Height of Feet	0.8 mm
Outer Diameter	13.6 mm
Inner Diameter	8.8 mm
Membrane Area	0.6 cm2
Membrane Pore Sizes	0.4, 8 µm
Solvent Compatibility	Same as polystyrene
Sterility	Gamma Irradiation, shipped sterile in 24-well plates
Membrane Types	Polyethelyene terephthalate (PET), Polytetrafluorethylene (PTFE), Polycarbonate (PC)

 

96-well Insert Plate Specifications
Receiver Plates	127.8 x 85.5 mm
Well Depth	12 mm
Membrane Diameter	4.29 mm
Membrane Area	0.11 cm2
Membrane Pore Sizes	0.4 µm
Solvent Compatibility	Same as polystyrene
Sterility	Gamma Irradiation, shipped sterile in 96-well plates
Membrane Types	Polyethelyene terephthalate (PET)

Membrane Types

• • Polycarbonate (PC)
  • Polyethylene terephthalate (PET)
  • Teflon (PTFE)
    • clear in culture
  • Specialty membranes also available

Coatings and Special Orders

MatTek offers PET, PC, PTFE as standard membrane options. Many cell types will grow directly in PermaCell Insert and Insert Plates. For cell types that require an extracellular matrix coating, we recommend the following special order coatings from Advanced BioMatrix:

PureCol® Type I Collagen https://advancedbiomatrix.com/purecol/

Type IV Collagen https://advancedbiomatrix.com/typeiv-lyophilized.html

Fibronectin https://advancedbiomatrix.com/fibronectin-solution.html

Vitronectin https://advancedbiomatrix.com/vitronectin-solution.html

Poly-D-Lysine https://advancedbiomatrix.com/polydlysinesolution.html

Poly-L-Lysine https://advancedbiomatrix.com/polyllysinesolution.html

Poly-L-Ornithine https://advancedbiomatrix.com/polylornithinesolution.html

 

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