FAQ’s

Glass Bottom Dishes

Dish Utilization

Q1: How are glass bottom dishes typically used?
Q2: What type of glass bottom dish should I use to grow my cells?
Q3: How can I search for published methods utilizing MatTek's glass bottom dishes?
Q4: What should I do if my cells won't grow or if I want to improve growth in the glass bottom dishes?
Q5: Can the coverslip be removed from the glass bottom dish?
Q6: How can I control the temperature of the glass bottom dishes for in situ (live) microscopy?
Q7: Why would one want to use glass bottom dishes containing gridded coverslips? What is the size of the grid? What if I can't see the grid?
Q8: How can I use the glass bottom dishes to look at tissue slices?
Q9: Can the glass bottom dishes be re-used?

Microscopy Types and Techniques

Q10: Are the glass bottom dishes good for confocal and fluorescence microscopy?
Q11: How can I use the glass bottom dishes for Nomarski Differential Interference Contrast (DIC) microscopy?
Q12: Can the dishes be used for high resolution techniques such as GSDIM, dSTORM, PALM , or TIRF microcopy?
Q13: Why are the 50 mm glass bottom dishes useful for microinjection and maintaining a constant atmosphere in the dish?
Q14:How can I perfuse the cells growing in the glass bottom dishes?

Dish Properties

Q15: What coverslip thickness should I use? What do the various coverslip numbers correspond to?
Q16: What is the adhesive used to attach the coverslips to the petri dishes?
Q17: How long can the dishes be stored?
Q18: Over what temperature range can the glass bottom dishes be used?
Q19: What are properties of the glass used in the glass bottom dishes?
Q20: How deep is the micro-well of the glass bottom dish?
Q21: What is the chemical compatibility of Glass Bottom Dishes and Multi-Well Plates? Can they be used with organic solvents?
Q22: How do I know that the glass bottom dishes are sterile?
Q23: How do the high tolerance P35G-0.170-14-C dishes differ from the standard P35G-1.5-14-C? For what applications would the P35G-0.170-14-C dishes be useful?

Coatings

Q24: What is the molecular weight range of the poly-d-lysine used to coat the PDL coated glass bottom dishes?
Q25: Why are the dishes coated with poly-d-lysine (instead of poly-l-lysine)?
Q26: What type of collagen is applied to the collagen coated glass bottom dishes (part #: P35GCol-x-xx-C or P50GCol-x-xx-F)?
Q27: Do the poly-lysine or collagen coatings affect the optical properties of the glass bottom dishes?

Special Orders

Q28: Other than the standard 10 mm, 14 mm, and 20 mm diameter glass surfaces, do the glass bottom dishes come with any alternative diameters?
Q29: What is the lead-time necessary for special orders? Is there a special order charge? Can special order items be returned?