Normal Human Epidermal Keratinocytes

MatTek’s primary Normal Human Epidermal Keratinocytes (NHEK) and Growth Medium (NHEK-GM) provide an optimized platform for investigating keratinocyte biology.   Available from neonatal or adult donors across a variety of ethnicities, MatTek’s NHEKs offer quality and convenience for the study of inflammation, oxidative stress, toxicity & phototoxicity, wound healing, drug screening and tissue modeling.

Donor matched cell types (fibroblasts & melanocytes) are available upon request.

MatTek’s NHEKs are isolated from neonatal (NHEK-CRY-NEO) or adult (NHEK-CRY-AD) donor tissue and cryopreserved as primary cells.  When cultured in MatTek’s NHEK-GM Growth Medium according to MatTek’s NHEK-CRY protocols, our epidermal keratinocytes are guaranteed to have ≥ 75% viability upon thaw and to produce ≥ 15 population doublings.  NHEKs are characterized by their cobblestone morphology and expression of cytokeratins.

Primary Human Epidermal Keratinocytes cultured in NHEK-GM were stained for Keratin 14 (pink), Phalloidin (blue) and Ki67 (yellow)
Primary Human Epidermal Keratinocytes cultured in NHEK-GM were stained for Keratin 14 (pink), Phalloidin (blue) and Ki67 (yellow)

Download the Human Epidermal Keratinocytes Data Sheet

Download the NHEK Antioxidant Application Note

 

 

Specifications Table_NHEK

All cells are negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected by PCR for all donors and cell lots.  This product is for research use only.  Not for use in animals, humans or diagnostic purposes.

 

MatTek’s cryopreserved cells are shipped on dry ice.  Cells are shipped every Monday, Tuesday and Wednesday.

Product Info Table

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