Human Dendritic Cells
Dendritic cells (DC) play a key role in the immunological reactions throughout the body. DC are highly specialized antigen-presenting cells (APC) located in the skin, mucosa, and lymphoid tissues. DC play a key role in the induction phase of contact allergenicity, and can be used in vitro to study contact sensitization and other immunological reactions of the body.
Autotologous T Cells (TC-100-CRY & TC-100-RT) are available upon request.
MatTek’s DCs are generated from CD34+ progenitor cells harvested from umbilical cord blood. The proprietary method used generates a large number of cells from each individual donor, allowing for multiple experiments in which the cell origin and functionality are constant. MatTek’s DCs express CD1a and HLA-DR and can be maintained in culture for at least 14 days without any significant changes in surface marker expression or functionality when cultured with DC-MM according to MatTek’s DC Culturing Protocol.
MatTek’s DCs were analyzed using transmission electron microscopy which show the presence of Birbeck granules, a key ultrastructural markers of DCs and LCs. Upon stimulation with LPS and PMA, a reproducible and significant induction is observed for IL-12, MIP-1α, MIP-3α and TNFα at the gene and protein expression levels. Furthermore, MatTek’s DCs are infectable with HIV and stimulate heterologous T Cells (TC) and autologous TCs following exposure to antigen.
All cells are negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected by PCR for all donors and cell lots. This product is for research use only. Not for use in animals, humans or diagnostic purposes.
MatTek’s DC cells (DC-100-CRY) are shipped cryopreserved on dry ice or as fresh cell suspensions at room temperature (DC-100-RT). Cells are shipped every Monday, Tuesday and Wednesday.