Ocular Irritation

EPIOCULAR
MatTek’s EpiOcular system consists of normal, human-derived epidermal keratinocytes which have been cultured to form a stratified, squamous epithelium similar to that found in the cornea. Cultured on specially prepared cell culture inserts using serum-free culture medium, the cells differentiate to form a multi-layered structure which closely parallels the corneal epithelium. Learn more about EpiOcular.

EPIOCULAR ASSAYS

MatTek offers several ocular irritation assays based on the EpiOcular tissue model.

EpiOcular Irritation Tests Graphic
1. EpiOcular Eye Irritation Test (EIT)

The EpiOcular EIT is an in vitro hazard assessment assay that has the ability to differentiate materials that are ocular non-irritants from materials that ocular irritants. The EIT is applicable to a broad range of organic and inorganic chemicals, and is accepted as OECD TG 492.

Download the EpiOcular EIT Protocol

Watch a video by Jove on EpiOcular EIT

2. EpiOcular “Sub-Draize” Ultra-mildness Test

The EpiOcular Ultra-mildness Test is an in vitro risk assessment assay routinely utilized for mild materials for which the Draize test is insensitive. This highly reproducible assay allows for quantifiable discrimination among mild, milder and mildest product formulations.

Download the EpiOcular Sub-Draize Protocol

3. EpiOcular MTT ET-50 (Neat)

This in vitro risk assessment assay is based on the effective time at which a material (applied neat) causes a 50% reduction in tissue viability (ET-50). Based on the ET-50, the test article is categorized into one of 4 classifications ranging from non-irritating to severe/extreme which correspond to groupings of Rabbit Draize Eye Scores (MMAS).

Download the EpiOcular MTT ET-50 (Neat) Protocol

4. EpiOcular MTT ET-50 (Dilution)

Similar to the MTT ET-50 Neat method, this in vitro risk assessment assay is based on the effective time at which a material (diluted to 20% in water) causes a 50% reduction in tissue viability (ET-50). Based on the ET-50, the test article is categorized into one of 4 classifications ranging from non-irritating to severe/extreme which correspond to groupings of Rabbit Draize Eye Scores (MMAS). This method is recommended for surfactant based solutions and is applicable to water-soluble materials with a specific gravity of > 0.95.

Download the EpiOcular MTT ET-50 (Dilution) Protocol

 

For more information, view Technical References.

MatTek’s EpiOcular system consists of normal, human-derived epidermal keratinocytes which have been cultured to form a stratified, squamous epithelium similar to that found in the cornea.